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P2RY10 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  P2RY10
Gene Name:  purinergic receptor P2Y, G-protein coupled, 10
Sequence             
Original Clone ID: ccsbBroad304_03030
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00445531

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1018nts         Open reading frame : 1 to 1018
Reference Sequence Alignment

ATGGCTAACCTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACT
GCTGAGATTTACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACC
TATATCCTCATATTCATTCCTGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGC
CGCTTCATCAGCAAGAAAAATAAAGCCATCATTTTCATGATCAACCTCTCTGTGGCTGAC
CTTGCTCATGTATTATCTTTACCCCTCCGGATTTACTATTACATCAGCCACCACTGGCCT
TTCCAGAGAGCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACATGTATGCCAGC
ATTTGTTTCCTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGG
GCCAGAGACTGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTG
GGGACTGCCTGTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCC
TGCTTTGCTGATCTTGGATACAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACA
GTTGCTGAGCTTGCAGGATTTGTGATCCCAGTGATCATCATCGCATGGTGTACCTGGAAA
ACTACTATATCCTTGAGACAGCCACCAATGGCTTTCCAAGGGATCAGTGAGAGGCAGAAA
GCACTGCGGATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCACTCCCTATCAT
ATTAACTTTATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTC
CGAATCGCACTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTG
GATCCAATTCTTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGC
AGTTCTGTGACCCGCTCCCGCCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : P2RY10
Symbol Nomenclature : P2RY10
SYNONYM : P2Y10
Designation : G-protein coupled purinergic receptor P2Y10|P2Y purinoceptor 10|P2Y-like receptor|putative P2Y purinoceptor 10
Full Nomenclature : purinergic receptor P2Y, G-protein coupled, 10
GENEID : 27334
Locus Tag : RP3-333E23.1
HGNC : 19906
MIM : 300529
Vega : OTTHUMG00000021896
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00445531        ---------------------------------------------------ATGGCTAAC
XM_005262124.1      ATGACAGCTTCAACTTTTAGACCACAGGCAAATGCTTTGAACCATAAATCCATGGCTAAC
NM_198333.1         ---------------------------------------------------ATGGCTAAC
NM_014499.2         ---------------------------------------------------ATGGCTAAC
XM_005262125.1      ---------------------------------------------------ATGGCTAAC
                                                                       *********

HsCD00445531        CTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATT
XM_005262124.1      CTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATT
NM_198333.1         CTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATT
NM_014499.2         CTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATT
XM_005262125.1      CTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATT
                    ************************************************************

HsCD00445531        TACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTC
XM_005262124.1      TACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTC
NM_198333.1         TACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTC
NM_014499.2         TACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTC
XM_005262125.1      TACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTC
                    ************************************************************

HsCD00445531        ATATTCATTCCTGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATC
XM_005262124.1      ATATTCATTCCTGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATC
NM_198333.1         ATATTCATTCCTGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATC
NM_014499.2         ATATTCATTCCTGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATC
XM_005262125.1      ATATTCATTCCTGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATC
                    ************************************************************

HsCD00445531        AGCAAGAAAAATAAAGCCATCATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCAT
XM_005262124.1      AGCAAGAAAAATAAAGCCATCATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCAT
NM_198333.1         AGCAAGAAAAATAAAGCCATCATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCAT
NM_014499.2         AGCAAGAAAAATAAAGCCATCATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCAT
XM_005262125.1      AGCAAGAAAAATAAAGCCATCATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCAT
                    ************************************************************

HsCD00445531        GTATTATCTTTACCCCTCCGGATTTACTATTACATCAGCCACCACTGGCCTTTCCAGAGA
XM_005262124.1      GTATTATCTTTACCCCTCCGGATTTACTATTACATCAGCCACCACTGGCCTTTCCAGAGA
NM_198333.1         GTATTATCTTTACCCCTCCGGATTTACTATTACATCAGCCACCACTGGCCTTTCCAGAGA
NM_014499.2         GTATTATCTTTACCCCTCCGGATTTACTATTACATCAGCCACCACTGGCCTTTCCAGAGA
XM_005262125.1      GTATTATCTTTACCCCTCCGGATTTACTATTACATCAGCCACCACTGGCCTTTCCAGAGA
                    ************************************************************

HsCD00445531        GCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACATGTATGCCAGCATTTGTTTC
XM_005262124.1      GCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACATGTATGCCAGCATTTGTTTC
NM_198333.1         GCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACATGTATGCCAGCATTTGTTTC
NM_014499.2         GCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACATGTATGCCAGCATTTGTTTC
XM_005262125.1      GCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACATGTATGCCAGCATTTGTTTC
                    ************************************************************

HsCD00445531        CTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGGGCCAGAGAC
XM_005262124.1      CTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGGGCCAGAGAC
NM_198333.1         CTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGGGCCAGAGAC
NM_014499.2         CTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGGGCCAGAGAC
XM_005262125.1      CTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGGGCCAGAGAC
                    ************************************************************

HsCD00445531        TGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCC
XM_005262124.1      TGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCC
NM_198333.1         TGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCC
NM_014499.2         TGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCC
XM_005262125.1      TGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCC
                    ************************************************************

HsCD00445531        TGTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCT
XM_005262124.1      TGTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCT
NM_198333.1         TGTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCT
NM_014499.2         TGTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCT
XM_005262125.1      TGTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCT
                    ************************************************************

HsCD00445531        GATCTTGGATACAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAG
XM_005262124.1      GATCTTGGATACAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAG
NM_198333.1         GATCTTGGATACAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAG
NM_014499.2         GATCTTGGATACAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAG
XM_005262125.1      GATCTTGGATACAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAG
                    ************************************************************

HsCD00445531        CTTGCAGGATTTGTGATCCCAGTGATCATCATCGCATGGTGTACCTGGAAAACTACTATA
XM_005262124.1      CTTGCAGGATTTGTGATCCCAGTGATCATCATCGCATGGTGTACCTGGAAAACTACTATA
NM_198333.1         CTTGCAGGATTTGTGATCCCAGTGATCATCATCGCATGGTGTACCTGGAAAACTACTATA
NM_014499.2         CTTGCAGGATTTGTGATCCCAGTGATCATCATCGCATGGTGTACCTGGAAAACTACTATA
XM_005262125.1      CTTGCAGGATTTGTGATCCCAGTGATCATCATCGCATGGTGTACCTGGAAAACTACTATA
                    ************************************************************

HsCD00445531        TCCTTGAGACAGCCACCAATGGCTTTCCAAGGGATCAGTGAGAGGCAGAAAGCACTGCGG
XM_005262124.1      TCCTTGAGACAGCCACCAATGGCTTTCCAAGGGATCAGTGAGAGGCAGAAAGCACTGCGG
NM_198333.1         TCCTTGAGACAGCCACCAATGGCTTTCCAAGGGATCAGTGAGAGGCAGAAAGCACTGCGG
NM_014499.2         TCCTTGAGACAGCCACCAATGGCTTTCCAAGGGATCAGTGAGAGGCAGAAAGCACTGCGG
XM_005262125.1      TCCTTGAGACAGCCACCAATGGCTTTCCAAGGGATCAGTGAGAGGCAGAAAGCACTGCGG
                    ************************************************************

HsCD00445531        ATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCACTCCCTATCATATTAACTTT
XM_005262124.1      ATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCACTCCCTATCATATTAACTTT
NM_198333.1         ATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCACTCCCTATCATATTAACTTT
NM_014499.2         ATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCACTCCCTATCATATTAACTTT
XM_005262125.1      ATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCACTCCCTATCATATTAACTTT
                    ************************************************************

HsCD00445531        ATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTCCGAATCGCA
XM_005262124.1      ATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTCCGAATCGCA
NM_198333.1         ATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTCCGAATCGCA
NM_014499.2         ATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTCCGAATCGCA
XM_005262125.1      ATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTCCGAATCGCA
                    ************************************************************

HsCD00445531        CTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATT
XM_005262124.1      CTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATT
NM_198333.1         CTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATT
NM_014499.2         CTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATT
XM_005262125.1      CTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATT
                    ************************************************************

HsCD00445531        CTTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTG
XM_005262124.1      CTTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTG
NM_198333.1         CTTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTG
NM_014499.2         CTTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTG
XM_005262125.1      CTTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTG
                    ************************************************************

HsCD00445531        ACCCGCTCCCGCCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGC---
XM_005262124.1      ACCCGCTCCCGCCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGCTAA
NM_198333.1         ACCCGCTCCCGCCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGCTAA
NM_014499.2         ACCCGCTCCCGCCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGCTAA
XM_005262125.1      ACCCGCTCCCGCCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGCTAA
                    ************************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 06670

Clone

Original Clone ID : ccsbBroad304_03030

Organism


TAX_ID : 9606
Species Specific ID: 27334

Genome

Chromosome : X
Map Location : Xq21.1
Ensembl : ENSG00000078589

Reagents

Labome : P2RY10-antibody