DNASU Plasmid Repository • 480.965.5697 | Email

APIP (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  APIP
Gene Name:  APAF1 interacting protein
Sequence             
Original Clone ID: ccsbBroad304_08211
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00446288

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 727nts         Open reading frame : 1 to 727
Reference Sequence Alignment

ATGTCTGGCTGTGATGCTTGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTATGATATAAATGAA
AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
GCTGTGATGGCCACCCTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGGA
ATTGTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : APIP
Symbol Nomenclature : APIP
SYNONYM : APIP2; CGI29; MMRP19; hAPIP
Designation : APAF1-interacting protein|MTRu-1-P dehydratase|methylthioribulose-1-phosphate dehydratase|probable methylthioribulose-1-phosphate dehydratase
Full Nomenclature : APAF1 interacting protein
GENEID : 51074
Locus Tag : CGI-29
GenBank Accession : HQ448158
HGNC : 17581
MIM : 612491
Vega : OTTHUMG00000166455
Target GenBank: HQ448158

 

Reference Sequence Alignment

  

HsCD00446288      ATGTCTGGCTGTGATGCTTGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
NM_015957.3       ATGTCTGGCTGTGATGCTCGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
                  ****************** *****************************************

HsCD00446288      AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
NM_015957.3       AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
                  ************************************************************

HsCD00446288      GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
NM_015957.3       GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
                  ************************************************************

HsCD00446288      TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTATGATATAAATGAA
NM_015957.3       TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTGTGATATAAATGAA
                  **********************************************.*************

HsCD00446288      AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
NM_015957.3       AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
                  ************************************************************

HsCD00446288      TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
NM_015957.3       TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
                  ************************************************************

HsCD00446288      GCTGTGATGGCCACCCTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
NM_015957.3       GCTGTGATGGCCACACTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
                  **************.*********************************************

HsCD00446288      ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
NM_015957.3       ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
                  ************************************************************

HsCD00446288      GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
NM_015957.3       GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
                  ************************************************************

HsCD00446288      ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
NM_015957.3       ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
                  ************************************************************

HsCD00446288      GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
NM_015957.3       GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
                  ************************************************************

HsCD00446288      GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGGA
NM_015957.3       GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGGA
                  ************************************************************

HsCD00446288      ATTGTC---
NM_015957.3       ATTGTCTAA
                  ******   

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Clone

Original Clone ID : ccsbBroad304_08211

Organism


TAX_ID : 9606
Species Specific ID: 51074

Genome

Chromosome : 11
Map Location : 11p13
Ensembl : ENSG00000149089

Reagents

Labome : APIP-antibody