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CAPN13 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN13
Gene Name:  calpain 13
Sequence             
Original Clone ID: ccsbBroad304_09334
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00446491

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2008nts         Open reading frame : 1 to 2008
Reference Sequence Alignment

ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGACC
GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCATGTCAAGATTTC
CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
GAGTGGATGAGCCTGGTCATGTACAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN13
Symbol Nomenclature : CAPN13
Designation : CANP 13|calcium-activated neutral proteinase 13|calpain-13
Full Nomenclature : calpain 13
GENEID : 92291
GenBank Accession : HQ258338
HGNC : 16663
MIM : 610228
Vega : OTTHUMG00000152053
Target GenBank: HQ258338

 

Reference Sequence Alignment

  

HsCD00446491      ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
NM_144575.2       ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
                  ************************************************************

HsCD00446491      TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
NM_144575.2       TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
                  ************************************************************

HsCD00446491      CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
NM_144575.2       CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
                  ************************************************************

HsCD00446491      ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
NM_144575.2       ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
                  ************************************************************

HsCD00446491      AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
NM_144575.2       AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
                  ************************************************************

HsCD00446491      TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
NM_144575.2       TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
                  ************************************************************

HsCD00446491      CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
NM_144575.2       CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
                  ************************************************************

HsCD00446491      ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
NM_144575.2       ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
                  ************************************************************

HsCD00446491      AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
NM_144575.2       AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
                  ************************************************************

HsCD00446491      TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
NM_144575.2       TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
                  ************************************************************

HsCD00446491      ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
NM_144575.2       ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
                  ************************************************************

HsCD00446491      AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
NM_144575.2       AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
                  ************************************************************

HsCD00446491      ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
NM_144575.2       ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
                  ************************************************************

HsCD00446491      TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGACC
NM_144575.2       TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGGCC
                  *********************************************************.**

HsCD00446491      GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
NM_144575.2       GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
                  ************************************************************

HsCD00446491      AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCATGTCAAGATTTC
NM_144575.2       AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCGTGTCAAGATTTC
                  ***********************************************.************

HsCD00446491      CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
NM_144575.2       CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
                  ************************************************************

HsCD00446491      AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
NM_144575.2       AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
                  ************************************************************

HsCD00446491      ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
NM_144575.2       ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
                  ************************************************************

HsCD00446491      GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
NM_144575.2       GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
                  ************************************************************

HsCD00446491      GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
NM_144575.2       GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
                  ************************************************************

HsCD00446491      AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
NM_144575.2       AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
                  ************************************************************

HsCD00446491      TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
NM_144575.2       TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
                  ************************************************************

HsCD00446491      CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
NM_144575.2       CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
                  ************************************************************

HsCD00446491      GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
NM_144575.2       GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
                  ************************************************************

HsCD00446491      TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
NM_144575.2       TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
                  ************************************************************

HsCD00446491      CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
NM_144575.2       CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
                  ************************************************************

HsCD00446491      GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
NM_144575.2       GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
                  ************************************************************

HsCD00446491      GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
NM_144575.2       GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
                  ************************************************************

HsCD00446491      CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
NM_144575.2       CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
                  ************************************************************

HsCD00446491      TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
NM_144575.2       TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
                  ************************************************************

HsCD00446491      GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
NM_144575.2       GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
                  ************************************************************

HsCD00446491      ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
NM_144575.2       ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
                  ************************************************************

HsCD00446491      GAGTGGATGAGCCTGGTCATGTACAAC---
NM_144575.2       GAGTGGATGAGCCTGGTCATGTACAACTGA
                  ***************************   

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
HPRD : 07008

Clone

Original Clone ID : ccsbBroad304_09334

Organism


TAX_ID : 9606
Species Specific ID: 92291

Genome

Chromosome : 2
Map Location : 2p22-p21
Ensembl : ENSG00000162949

Reagents

Labome : CAPN13-antibody