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CCT6A (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CCT6A
Gene Name:  chaperonin containing TCP1, subunit 6A (zeta 1)
Sequence             
Original Clone ID: ccsbBroad304_00241
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00446630

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1594nts         Open reading frame : 1 to 1594
Reference Sequence Alignment

ATGGCGGCGGTGAAGACCCTGAACCCCAAGGCCGAGGTGGCCCGAGCGCAGGCGGCGCTG
GCGGTCAACATCAGCGCAGCGCGGGGTCTGCAGGACGTGCTAAGGACCAACCTGGGGCCC
AAGGGCACCATGAAGATGCTCGTTTCTGGCGCTGGAGACATCAAACTTACTAAAGACGGC
AATGTGCTGCTTCACGAAATGCAAATTCAACACCCAACAGCTTCCTTAATAGCAAAGGTA
GCAACAGCCCAGGATGATATAACTGGTGATGGTACGACTTCTAATGTCCTAATCATTGGA
GAGCTGCTGAAACAGGCGGATCTCTACATTTCTGAAGGCCTTCATCCTAGAATAATCACT
GAAGGATTTGAAGCTGCAAAGGAAAAGGCCCTTCAGTTTTTGGAAGAAGTCAAAGTAAGC
AGAGAGATGGACAGGGAAACACTTATAGATGTGGCCAGAACATCTCTTCGTACTAAAGTT
CATGCTGAACTTGCAGATGTCTTAACAGAGGCTGTAGTGGACTCCATTTTGGCCATTAAA
AAGCAAGATGAACCTATTGATCTCTTCATGATTGAGATCATGGAGATGAAACATAAATCT
GAAACTGATACAAGCTTAATCAGAGGGCTTGTTTTGGACCACGGAGCACGGCATCCTGAT
ATGAAGAAAAGGGTGGAGGATGCATACATCCTCACTTGTAACGTGTCATTAGAGTATGAG
AAAACAGAAGTGAATTCTGGCTTTTTTTACAAGAGTGCAGAAGAGAGAGAAAAACTCGTG
AAAGCTGAAAGAAAATTCATTGAAGATAGGGTTAAAAAAATAATAGAACTGAAAAGGAAA
GTCTGTGGCGATTCAGATAAAGGATTTGTTGTTATTAATCAAAAGGGAATTGACCCCTTT
TCCTTAGATGCTCTTTCAAAAGAAGGCATAGTTGCTCTGCGCAGAGCTAAAAGGAGAAAT
ATGGAGAGGCTGACTCTTGCTTGTGGTGGGGTAGCCCTGAATTCTTTTGACGACCTAAGT
CCTGACTGCTTGGGACATGCAGGACTTGTATATGAGTATACATTGGGAGAAGAGAAGTTT
ACCTTTATTGAGAAATGTAACAACCCTCGTTCTGTCACATTATTGATCAAAGGACCAAAT
AAGCACACACTCACTCAGATCAAAGATGCAGTGAGGGACGGCTTGAGGGCTGTCAAAAAT
GCTATTGATGATGGCTGTGTGGTTCCAGGTGCTGGTGCCGTGGAAGTGGCAATGGCAGAA
GCCCTGATTAAACATAAGCCCAGTGTAAAGGGCAGGGCACAGCTTGGAGTCCAAGCATTT
GCTGATGCATTGCTCATTATTCCCAAGGTTCTTGCTCAGAACTCTGGTTTTGACCTTCAG
GAAACATTAGTTAAAATTCAAGCAGAACATTCAGAATCAGGTCAGCTTGTGGGTGTGGAC
CTGAACACAGGTGAGCCAATGGTGGCAGCAGAAGTAGGCGTATGGGATAACTATTGTGTA
AAGAAACAGCTTCTTCACTCCTGCACTGTGATTGCCACCAACATTCTCTTGGTTGATGAG
ATCATGCGAGCTGGAATGTCTTCTCTGAAAGGT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CCT6A
Symbol Nomenclature : CCT6A
SYNONYM : CCT-zeta; CCT-zeta-1; CCT6; Cctz; HTR3; MoDP-2; TCP-1-zeta; TCP20; TCPZ; TTCP20
Designation : T-complex protein 1 subunit zeta|T-complex protein 1, zeta subunit|acute morphine dependence related protein 2|acute morphine dependence-related protein 2|amino acid transport defect-complementing|chaperonin containing T-complex subunit 6|histidine transport regulator 3
Full Nomenclature : chaperonin containing TCP1, subunit 6A (zeta 1)
GENEID : 908
HGNC : 1620
MIM : 104613
Vega : OTTHUMG00000022842
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00446630        ATGGCGGCGGTGAAGACCCTGAACCCCAAGGCCGAGGTGGCCCGAGCGCAGGCGGCGCTG
NM_001762.3         ATGGCGGCGGTGAAGACCCTGAACCCCAAGGCCGAGGTGGCCCGAGCGCAGGCGGCGCTG
NM_001009186.1      ATGGCGGCGGTGAAGACCCTGAACCCCAAGGCCGAGGTGGCCCGAGCGCAGGCGGCGCTG
                    ************************************************************

HsCD00446630        GCGGTCAACATCAGCGCAGCGCGGGGTCTGCAGGACGTGCTAAGGACCAACCTGGGGCCC
NM_001762.3         GCGGTCAACATCAGCGCAGCGCGGGGTCTGCAGGACGTGCTAAGGACCAACCTGGGGCCC
NM_001009186.1      GCGGTCAACATCAGCGCAGCGCGGGGTCTGCAGGACGTGCTAAGGACCAACCTGGGGCCC
                    ************************************************************

HsCD00446630        AAGGGCACCATGAAGATGCTCGTTTCTGGCGCTGGAGACATCAAACTTACTAAAGACGGC
NM_001762.3         AAGGGCACCATGAAGATGCTCGTTTCTGGCGCTGGAGACATCAAACTTACTAAAGACGGC
NM_001009186.1      AAGGGCACCATGAAGATGCTCGTTTCTGGCGCTGGAGACATCAAACTTACTAAAGACGGC
                    ************************************************************

HsCD00446630        AATGTGCTGCTTCACGAAATGCAAATTCAACACCCAACAGCTTCCTTAATAGCAAAGGTA
NM_001762.3         AATGTGCTGCTTCACGAAATGCAAATTCAACACCCAACAGCTTCCTTAATAGCAAAGGTA
NM_001009186.1      AATGTGCTGCTTCACGAAAT----------------------------------------
                    ********************                                        

HsCD00446630        GCAACAGCCCAGGATGATATAACTGGTGATGGTACGACTTCTAATGTCCTAATCATTGGA
NM_001762.3         GCAACAGCCCAGGATGATATAACTGGTGATGGTACGACTTCTAATGTCCTAATCATTGGA
NM_001009186.1      ------------------------------------------------------------
                                                                                

HsCD00446630        GAGCTGCTGAAACAGGCGGATCTCTACATTTCTGAAGGCCTTCATCCTAGAATAATCACT
NM_001762.3         GAGCTGCTGAAACAGGCGGATCTCTACATTTCTGAAGGCCTTCATCCTAGAATAATCACT
NM_001009186.1      -----------------------------------GGGCCTTCATCCTAGAATAATCACT
                                                       .************************

HsCD00446630        GAAGGATTTGAAGCTGCAAAGGAAAAGGCCCTTCAGTTTTTGGAAGAAGTCAAAGTAAGC
NM_001762.3         GAAGGATTTGAAGCTGCAAAGGAAAAGGCCCTTCAGTTTTTGGAAGAAGTCAAAGTAAGC
NM_001009186.1      GAAGGATTTGAAGCTGCAAAGGAAAAGGCCCTTCAGTTTTTGGAAGAAGTCAAAGTAAGC
                    ************************************************************

HsCD00446630        AGAGAGATGGACAGGGAAACACTTATAGATGTGGCCAGAACATCTCTTCGTACTAAAGTT
NM_001762.3         AGAGAGATGGACAGGGAAACACTTATAGATGTGGCCAGAACATCTCTTCGTACTAAAGTT
NM_001009186.1      AGAGAGATGGACAGGGAAACACTTATAGATGTGGCCAGAACATCTCTTCGTACTAAAGTT
                    ************************************************************

HsCD00446630        CATGCTGAACTTGCAGATGTCTTAACAGAGGCTGTAGTGGACTCCATTTTGGCCATTAAA
NM_001762.3         CATGCTGAACTTGCAGATGTCTTAACAGAGGCTGTAGTGGACTCCATTTTGGCCATTAAA
NM_001009186.1      CATGCTGAACTTGCAGATGTCTTAACAGAGGCTGTAGTGGACTCCATTTTGGCCATTAAA
                    ************************************************************

HsCD00446630        AAGCAAGATGAACCTATTGATCTCTTCATGATTGAGATCATGGAGATGAAACATAAATCT
NM_001762.3         AAGCAAGATGAACCTATTGATCTCTTCATGATTGAGATCATGGAGATGAAACATAAATCT
NM_001009186.1      AAGCAAGATGAACCTATTGATCTCTTCATGATTGAGATCATGGAGATGAAACATAAATCT
                    ************************************************************

HsCD00446630        GAAACTGATACAAGCTTAATCAGAGGGCTTGTTTTGGACCACGGAGCACGGCATCCTGAT
NM_001762.3         GAAACTGATACAAGCTTAATCAGAGGGCTTGTTTTGGACCACGGAGCACGGCATCCTGAT
NM_001009186.1      GAAACTGATACAAGCTTAATCAGAGGGCTTGTTTTGGACCACGGAGCACGGCATCCTGAT
                    ************************************************************

HsCD00446630        ATGAAGAAAAGGGTGGAGGATGCATACATCCTCACTTGTAACGTGTCATTAGAGTATGAG
NM_001762.3         ATGAAGAAAAGGGTGGAGGATGCATACATCCTCACTTGTAACGTGTCATTAGAGTATGAG
NM_001009186.1      ATGAAGAAAAGGGTGGAGGATGCATACATCCTCACTTGTAACGTGTCATTAGAGTATGAG
                    ************************************************************

HsCD00446630        AAAACAGAAGTGAATTCTGGCTTTTTTTACAAGAGTGCAGAAGAGAGAGAAAAACTCGTG
NM_001762.3         AAAACAGAAGTGAATTCTGGCTTTTTTTACAAGAGTGCAGAAGAGAGAGAAAAACTCGTG
NM_001009186.1      AAAACAGAAGTGAATTCTGGCTTTTTTTACAAGAGTGCAGAAGAGAGAGAAAAACTCGTG
                    ************************************************************

HsCD00446630        AAAGCTGAAAGAAAATTCATTGAAGATAGGGTTAAAAAAATAATAGAACTGAAAAGGAAA
NM_001762.3         AAAGCTGAAAGAAAATTCATTGAAGATAGGGTTAAAAAAATAATAGAACTGAAAAGGAAA
NM_001009186.1      AAAGCTGAAAGAAAATTCATTGAAGATAGGGTTAAAAAAATAATAGAACTGAAAAGGAAA
                    ************************************************************

HsCD00446630        GTCTGTGGCGATTCAGATAAAGGATTTGTTGTTATTAATCAAAAGGGAATTGACCCCTTT
NM_001762.3         GTCTGTGGCGATTCAGATAAAGGATTTGTTGTTATTAATCAAAAGGGAATTGACCCCTTT
NM_001009186.1      GTCTGTGGCGATTCAGATAAAGGATTTGTTGTTATTAATCAAAAGGGAATTGACCCCTTT
                    ************************************************************

HsCD00446630        TCCTTAGATGCTCTTTCAAAAGAAGGCATAGTTGCTCTGCGCAGAGCTAAAAGGAGAAAT
NM_001762.3         TCCTTAGATGCTCTTTCAAAAGAAGGCATAGTTGCTCTGCGCAGAGCTAAAAGGAGAAAT
NM_001009186.1      TCCTTAGATGCTCTTTCAAAAGAAGGCATAGTTGCTCTGCGCAGAGCTAAAAGGAGAAAT
                    ************************************************************

HsCD00446630        ATGGAGAGGCTGACTCTTGCTTGTGGTGGGGTAGCCCTGAATTCTTTTGACGACCTAAGT
NM_001762.3         ATGGAGAGGCTGACTCTTGCTTGTGGTGGGGTAGCCCTGAATTCTTTTGACGACCTAAGT
NM_001009186.1      ATGGAGAGGCTGACTCTTGCTTGTGGTGGGGTAGCCCTGAATTCTTTTGACGACCTAAGT
                    ************************************************************

HsCD00446630        CCTGACTGCTTGGGACATGCAGGACTTGTATATGAGTATACATTGGGAGAAGAGAAGTTT
NM_001762.3         CCTGACTGCTTGGGACATGCAGGACTTGTATATGAGTATACATTGGGAGAAGAGAAGTTT
NM_001009186.1      CCTGACTGCTTGGGACATGCAGGACTTGTATATGAGTATACATTGGGAGAAGAGAAGTTT
                    ************************************************************

HsCD00446630        ACCTTTATTGAGAAATGTAACAACCCTCGTTCTGTCACATTATTGATCAAAGGACCAAAT
NM_001762.3         ACCTTTATTGAGAAATGTAACAACCCTCGTTCTGTCACATTATTGATCAAAGGACCAAAT
NM_001009186.1      ACCTTTATTGAGAAATGTAACAACCCTCGTTCTGTCACATTATTGATCAAAGGACCAAAT
                    ************************************************************

HsCD00446630        AAGCACACACTCACTCAGATCAAAGATGCAGTGAGGGACGGCTTGAGGGCTGTCAAAAAT
NM_001762.3         AAGCACACACTCACTCAGATCAAAGATGCAGTGAGGGACGGCTTGAGGGCTGTCAAAAAT
NM_001009186.1      AAGCACACACTCACTCAGATCAAAGATGCAGTGAGGGACGGCTTGAGGGCTGTCAAAAAT
                    ************************************************************

HsCD00446630        GCTATTGATGATGGCTGTGTGGTTCCAGGTGCTGGTGCCGTGGAAGTGGCAATGGCAGAA
NM_001762.3         GCTATTGATGATGGCTGTGTGGTTCCAGGTGCTGGTGCCGTGGAAGTGGCAATGGCAGAA
NM_001009186.1      GCTATTGATGATGGCTGTGTGGTTCCAGGTGCTGGTGCCGTGGAAGTGGCAATGGCAGAA
                    ************************************************************

HsCD00446630        GCCCTGATTAAACATAAGCCCAGTGTAAAGGGCAGGGCACAGCTTGGAGTCCAAGCATTT
NM_001762.3         GCCCTGATTAAACATAAGCCCAGTGTAAAGGGCAGGGCACAGCTTGGAGTCCAAGCATTT
NM_001009186.1      GCCCTGATTAAACATAAGCCCAGTGTAAAGGGCAGGGCACAGCTTGGAGTCCAAGCATTT
                    ************************************************************

HsCD00446630        GCTGATGCATTGCTCATTATTCCCAAGGTTCTTGCTCAGAACTCTGGTTTTGACCTTCAG
NM_001762.3         GCTGATGCATTGCTCATTATTCCCAAGGTTCTTGCTCAGAACTCTGGTTTTGACCTTCAG
NM_001009186.1      GCTGATGCATTGCTCATTATTCCCAAGGTTCTTGCTCAGAACTCTGGTTTTGACCTTCAG
                    ************************************************************

HsCD00446630        GAAACATTAGTTAAAATTCAAGCAGAACATTCAGAATCAGGTCAGCTTGTGGGTGTGGAC
NM_001762.3         GAAACATTAGTTAAAATTCAAGCAGAACATTCAGAATCAGGTCAGCTTGTGGGTGTGGAC
NM_001009186.1      GAAACATTAGTTAAAATTCAAGCAGAACATTCAGAATCAGGTCAGCTTGTGGGTGTGGAC
                    ************************************************************

HsCD00446630        CTGAACACAGGTGAGCCAATGGTGGCAGCAGAAGTAGGCGTATGGGATAACTATTGTGTA
NM_001762.3         CTGAACACAGGTGAGCCAATGGTGGCAGCAGAAGTAGGCGTATGGGATAACTATTGTGTA
NM_001009186.1      CTGAACACAGGTGAGCCAATGGTGGCAGCAGAAGTAGGCGTATGGGATAACTATTGTGTA
                    ************************************************************

HsCD00446630        AAGAAACAGCTTCTTCACTCCTGCACTGTGATTGCCACCAACATTCTCTTGGTTGATGAG
NM_001762.3         AAGAAACAGCTTCTTCACTCCTGCACTGTGATTGCCACCAACATTCTCTTGGTTGATGAG
NM_001009186.1      AAGAAACAGCTTCTTCACTCCTGCACTGTGATTGCCACCAACATTCTCTTGGTTGATGAG
                    ************************************************************

HsCD00446630        ATCATGCGAGCTGGAATGTCTTCTCTGAAAGGT---
NM_001762.3         ATCATGCGAGCTGGAATGTCTTCTCTGAAAGGTTGA
NM_001009186.1      ATCATGCGAGCTGGAATGTCTTCTCTGAAAGGTTGA
                    *********************************   

Pathways

Reactome : Association of TriC/CCT with target proteins during biosynthesis
Reactome : Chaperonin-mediated protein folding
Reactome : Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding
Reactome : Folding of actin by CCT/TriC
Reactome : Formation of tubulin folding intermediates by CCT/TriC
Reactome : Metabolism of proteins
Reactome : Prefoldin mediated transfer of substrate to CCT/TriC
Reactome : Protein folding

Protein

HPRD : 00089

Clone

Original Clone ID : ccsbBroad304_00241

Organism


TAX_ID : 9606
Species Specific ID: 908

Genome

Chromosome : 7
Map Location : 7p11.2
Ensembl : ENSG00000146731

Reagents

Labome : CCT6A-antibody