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POLB (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  POLB
Gene Name:  polymerase (DNA directed), beta
Sequence             
Original Clone ID: ccsbBroad304_01234
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00446756

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1006nts         Open reading frame : 1 to 1006
Reference Sequence Alignment

ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
ACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTG
AAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGAT
ATTGTACTAAATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGT
TTCAGAAGAGGTGCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTC
ACTTCAGAATCAACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAG
GTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAG
CTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATA
CCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAG
AATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCC
TTGGGAGTCACTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTT
GATTACATCCAGTGGAAATACCGGGAACCCAAGGACCGGAGCGAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : POLB
Symbol Nomenclature : POLB
Designation : DNA pol beta|DNA polymerase beta|DNA polymerase beta subunit|mutant DNA polymerase beta
Full Nomenclature : polymerase (DNA directed), beta
GENEID : 5423
HGNC : 9174
MIM : 174760
Vega : OTTHUMG00000164093
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00446756        ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
XM_005273537.1      ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
XM_005273535.1      ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
NM_002690.2         ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
                    ************************************************************

HsCD00446756        GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
XM_005273537.1      GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
XM_005273535.1      GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
NM_002690.2         GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
                    ************************************************************

HsCD00446756        AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
XM_005273537.1      AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
XM_005273535.1      AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
NM_002690.2         AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
                    ************************************************************

HsCD00446756        AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
XM_005273537.1      AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
XM_005273535.1      AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
NM_002690.2         AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
                    ************************************************************

HsCD00446756        AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
XM_005273537.1      AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
XM_005273535.1      AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
NM_002690.2         AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
                    ************************************************************

HsCD00446756        ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
XM_005273537.1      ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
XM_005273535.1      ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
NM_002690.2         ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
                    ************************************************************

HsCD00446756        ACACTAGAAG--------------------------------------------------
XM_005273537.1      ACACTAGAAG--------------------------------------------------
XM_005273535.1      ACACTAGAAGGCTCACAGCTGGATTCATGCCCAGTAAAGGGACACCTGAATGGAACTGAG
NM_002690.2         ACACTAGAAG--------------------------------------------------
                    **********                                                  

HsCD00446756        -------------------------------------------------------ATCTC
XM_005273537.1      -------------------------------------------------------ATCTC
XM_005273535.1      TCACTTTTAGACTTAATATGGGATGTTATGACAATTCTTAAGTTAAAAAATGCAGATCTC
NM_002690.2         -------------------------------------------------------ATCTC
                                                                           *****

HsCD00446756        AGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGGGAC
XM_005273537.1      AGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGGGAC
XM_005273535.1      AGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGGGAC
NM_002690.2         AGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGGGAC
                    ************************************************************

HsCD00446756        TTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAATGAA
XM_005273537.1      TTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAATGAA
XM_005273535.1      TTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAATGAA
NM_002690.2         TTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAATGAA
                    ************************************************************

HsCD00446756        GTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTGCA
XM_005273537.1      GTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTGCA
XM_005273535.1      GTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTGCA
NM_002690.2         GTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTGCA
                    ************************************************************

HsCD00446756        GAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCAACC
XM_005273537.1      GAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCAACC
XM_005273535.1      GAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCAACC
NM_002690.2         GAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCAACC
                    ************************************************************

HsCD00446756        AAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATCACA
XM_005273537.1      AAACAG------------------------------------------------------
XM_005273535.1      AAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATCACA
NM_002690.2         AAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATCACA
                    ******                                                      

HsCD00446756        GATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAAAAT
XM_005273537.1      ---------------------------------GGTGTTTGCCAGCTTCCCAGTAAAAAT
XM_005273535.1      GATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAAAAT
NM_002690.2         GATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAAAAT
                                                     ***************************

HsCD00446756        GATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAGTAT
XM_005273537.1      GATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAGTAT
XM_005273535.1      GATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAGTAT
NM_002690.2         GATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAGTAT
                    ************************************************************

HsCD00446756        TACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCTCAT
XM_005273537.1      TACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCTCAT
XM_005273535.1      TACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCTCAT
NM_002690.2         TACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCTCAT
                    ************************************************************

HsCD00446756        GCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACTGGA
XM_005273537.1      GCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACTGGA
XM_005273535.1      GCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACTGGA
NM_002690.2         GCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACTGGA
                    ************************************************************

HsCD00446756        GTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAGTGG
XM_005273537.1      GTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAGTGG
XM_005273535.1      GTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAGTGG
NM_002690.2         GTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAGTGG
                    ************************************************************

HsCD00446756        AAATACCGGGAACCCAAGGACCGGAGCGAA---
XM_005273537.1      AAATACCGGGAACCCAAGGACCGGAGCGAATGA
XM_005273535.1      AAATACCGGGAACCCAAGGACCGGAGCGAATGA
NM_002690.2         AAATACCGGGAACCCAAGGACCGGAGCGAATGA
                    ******************************   

Pathways

Reactome : Base Excision Repair
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : DNA Repair
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
Reactome : Resolution of Abasic Sites (AP sites)
WikiPathway : Nucleotide Metabolism

Protein

SMART domain : POLXc : DNA polymerase X family
HPRD : 07517

Clone

Original Clone ID : ccsbBroad304_01234

Organism


TAX_ID : 9606
Species Specific ID: 5423

Genome

Chromosome : 8
Map Location : 8p11.2
Ensembl : ENSG00000070501