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SLC10A6 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SLC10A6
Gene Name:  solute carrier family 10 (sodium/bile acid cotransporter), member 6
Sequence             
Original Clone ID: ccsbBroad304_10050
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00446789

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1132nts         Open reading frame : 1 to 1132
Reference Sequence Alignment

ATGAGAGCCAATTGTTCCAGCAGCTCAGCCTGCCCTGCCAACAGTTCAGAGGAGGAGCTG
CCAGTGGGACTGGAGGTGCATGGAAACCTGGAGCTCGTTTTCACAGTGGTGTCCACTGTG
ATGATGGGGCTGCTCATGTTCTCTTTGGGATGTTCCGTGGAGATCCGGAAGCTGTGGTCG
CACATCAGGAGACCCTGGGGCATTGCTGTGGGACTGCTCTGCCAGTTTGGGCTCATGCCT
TTTACAGCTTATCTCCTGGCCATTAGCTTTTCTCTGAAGCCAGTCCAAGCTATTGCTGTT
CTCATCATGGGCTGCTGCCCGGGGGGCACCATCTCTAACGTTTTCACCTTCTGGGTTGAT
GGAGATATGGATCTCAGCATCAGTATGACAACCTGTTCCACCGTGGCCGCCCTGGGAATG
ATGCCACTCTGCATTTATCTCTACACCTGGTCCTGGAGTCTTCAGCAGAATCTCACCATT
CCTTATCAGAACATAGGAATTACCCTTGTGTGCCTGACCATTCCTGTGGCCTTTGGTGTC
TATGTGAATTACAGATGGCCAAAACAATCCAAAATCATTCTCAAGATTGGGGCCGTTGTT
GGTGGGGTCCTCCTTCTGGTGGTCGCAGTTGCTGGTGTGGTCCTGGCGAAAGGATCTTGG
AATTCAGACATCACCCTTCTGACCATCAGTTTCATCTTTCCTTTGATTGGCCATGTCACG
GGTTTTCTGCTGGCACTTTTTACCCACCAGTCTTGGCAAAGGTGCAGGACAATTTCCTTA
GAAACTGGAGCTCAGAATATTCAGATGTGCATCACCATGCTCCAGTTATCTTTCACTGCT
GAGCACTTGGTCCAGATGTTGAGTTTCCCACTGGCCTATGGACTCTTCCAGCTGATAGAT
GGATTTCTTATTGTTGCAGCATATCAGACGTACAAGAGGAGATTGAAGAACAAACATGGA
AAAAAGAACTCAGGTTGCACAGAAGTCTGCCATACGAGGAAATCGACTTCTTCCAGAGAG
ACCAATGCCTTCTTGGAGGTGAATGAAGAAGGTGCCATCACTCCTGGGCCACCAGGGCCA
ATGGATTGCCACAGGGCTCTCGAGCCAGTTGGCCACATCACTTCATGTGAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SLC10A6
Symbol Nomenclature : SLC10A6
SYNONYM : SOAT
Designation : sodium-dependent organic anion transporter|solute carrier family 10 (sodium/bile acid cotransporter family), member 6|solute carrier family 10 member 6
Full Nomenclature : solute carrier family 10 (sodium/bile acid cotransporter), member 6
GENEID : 345274
HGNC : 30603
MIM : 613366
Vega : OTTHUMG00000130596
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00446789      ATGAGAGCCAATTGTTCCAGCAGCTCAGCCTGCCCTGCCAACAGTTCAGAGGAGGAGCTG
NM_197965.2       ATGAGAGCCAATTGTTCCAGCAGCTCAGCCTGCCCTGCCAACAGTTCAGAGGAGGAGCTG
                  ************************************************************

HsCD00446789      CCAGTGGGACTGGAGGTGCATGGAAACCTGGAGCTCGTTTTCACAGTGGTGTCCACTGTG
NM_197965.2       CCAGTGGGACTGGAGGTGCATGGAAACCTGGAGCTCGTTTTCACAGTGGTGTCCACTGTG
                  ************************************************************

HsCD00446789      ATGATGGGGCTGCTCATGTTCTCTTTGGGATGTTCCGTGGAGATCCGGAAGCTGTGGTCG
NM_197965.2       ATGATGGGGCTGCTCATGTTCTCTTTGGGATGTTCCGTGGAGATCCGGAAGCTGTGGTCG
                  ************************************************************

HsCD00446789      CACATCAGGAGACCCTGGGGCATTGCTGTGGGACTGCTCTGCCAGTTTGGGCTCATGCCT
NM_197965.2       CACATCAGGAGACCCTGGGGCATTGCTGTGGGACTGCTCTGCCAGTTTGGGCTCATGCCT
                  ************************************************************

HsCD00446789      TTTACAGCTTATCTCCTGGCCATTAGCTTTTCTCTGAAGCCAGTCCAAGCTATTGCTGTT
NM_197965.2       TTTACAGCTTATCTCCTGGCCATTAGCTTTTCTCTGAAGCCAGTCCAAGCTATTGCTGTT
                  ************************************************************

HsCD00446789      CTCATCATGGGCTGCTGCCCGGGGGGCACCATCTCTAACGTTTTCACCTTCTGGGTTGAT
NM_197965.2       CTCATCATGGGCTGCTGCCCGGGGGGCACCATCTCTAACATTTTCACCTTCTGGGTTGAT
                  ***************************************.********************

HsCD00446789      GGAGATATGGATCTCAGCATCAGTATGACAACCTGTTCCACCGTGGCCGCCCTGGGAATG
NM_197965.2       GGAGATATGGATCTCAGCATCAGTATGACAACCTGTTCCACCGTGGCCGCCCTGGGAATG
                  ************************************************************

HsCD00446789      ATGCCACTCTGCATTTATCTCTACACCTGGTCCTGGAGTCTTCAGCAGAATCTCACCATT
NM_197965.2       ATGCCACTCTGCATTTATCTCTACACCTGGTCCTGGAGTCTTCAGCAGAATCTCACCATT
                  ************************************************************

HsCD00446789      CCTTATCAGAACATAGGAATTACCCTTGTGTGCCTGACCATTCCTGTGGCCTTTGGTGTC
NM_197965.2       CCTTATCAGAACATAGGAATTACCCTTGTGTGCCTGACCATTCCTGTGGCCTTTGGTGTC
                  ************************************************************

HsCD00446789      TATGTGAATTACAGATGGCCAAAACAATCCAAAATCATTCTCAAGATTGGGGCCGTTGTT
NM_197965.2       TATGTGAATTACAGATGGCCAAAACAATCCAAAATCATTCTCAAGATTGGGGCCGTTGTT
                  ************************************************************

HsCD00446789      GGTGGGGTCCTCCTTCTGGTGGTCGCAGTTGCTGGTGTGGTCCTGGCGAAAGGATCTTGG
NM_197965.2       GGTGGGGTCCTCCTTCTGGTGGTCGCAGTTGCTGGTGTGGTCCTGGCGAAAGGATCTTGG
                  ************************************************************

HsCD00446789      AATTCAGACATCACCCTTCTGACCATCAGTTTCATCTTTCCTTTGATTGGCCATGTCACG
NM_197965.2       AATTCAGACATCACCCTTCTGACCATCAGTTTCATCTTTCCTTTGATTGGCCATGTCACG
                  ************************************************************

HsCD00446789      GGTTTTCTGCTGGCACTTTTTACCCACCAGTCTTGGCAAAGGTGCAGGACAATTTCCTTA
NM_197965.2       GGTTTTCTGCTGGCACTTTTTACCCACCAGTCTTGGCAAAGGTGCAGGACAATTTCCTTA
                  ************************************************************

HsCD00446789      GAAACTGGAGCTCAGAATATTCAGATGTGCATCACCATGCTCCAGTTATCTTTCACTGCT
NM_197965.2       GAAACTGGAGCTCAGAATATTCAGATGTGCATCACCATGCTCCAGTTATCTTTCACTGCT
                  ************************************************************

HsCD00446789      GAGCACTTGGTCCAGATGTTGAGTTTCCCACTGGCCTATGGACTCTTCCAGCTGATAGAT
NM_197965.2       GAGCACTTGGTCCAGATGTTGAGTTTCCCACTGGCCTATGGACTCTTCCAGCTGATAGAT
                  ************************************************************

HsCD00446789      GGATTTCTTATTGTTGCAGCATATCAGACGTACAAGAGGAGATTGAAGAACAAACATGGA
NM_197965.2       GGATTTCTTATTGTTGCAGCATATCAGACGTACAAGAGGAGATTGAAGAACAAACATGGA
                  ************************************************************

HsCD00446789      AAAAAGAACTCAGGTTGCACAGAAGTCTGCCATACGAGGAAATCGACTTCTTCCAGAGAG
NM_197965.2       AAAAAGAACTCAGGTTGCACAGAAGTCTGCCATACGAGGAAATCGACTTCTTCCAGAGAG
                  ************************************************************

HsCD00446789      ACCAATGCCTTCTTGGAGGTGAATGAAGAAGGTGCCATCACTCCTGGGCCACCAGGGCCA
NM_197965.2       ACCAATGCCTTCTTGGAGGTGAATGAAGAAGGTGCCATCACTCCTGGGCCACCAGGGCCA
                  ************************************************************

HsCD00446789      ATGGATTGCCACAGGGCTCTCGAGCCAGTTGGCCACATCACTTCATGTGAA---
NM_197965.2       ATGGATTGCCACAGGGCTCTCGAGCCAGTTGGCCACATCACTTCATGTGAATAG
                  ***************************************************   

Pathways

Reactome : Bile acid and bile salt metabolism
Reactome : Bile salt and organic anion SLC transporters
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Recycling of bile acids and salts
Reactome : SLC-mediated transmembrane transport
Reactome : Transmembrane transport of small molecules
Reactome : Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds

Protein

HPRD : 11593

Clone

Original Clone ID : ccsbBroad304_10050

Organism


TAX_ID : 9606
Species Specific ID: 345274

Genome

Chromosome : 4
Map Location : 4q21.3
Ensembl : ENSG00000145283

Reagents

Labome : SLC10A6-antibody