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CSTF1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CSTF1
Gene Name:  cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH188986.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00044759

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1296nts         Open reading frame : 1 to 1296
Reference Sequence Alignment

ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
GCGCGGTTTTGGTACCGGAGATCGACCACTGACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1394
Start on reference sequence 99
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSTF1
Symbol Nomenclature : CSTF1
SYNONYM : CstF-50; CstFp50
Designation : CF-1 50 kDa subunit|CSTF 50 kDa subunit|cleavage stimulation factor 50 kDa subunit|cleavage stimulation factor subunit 1
Full Nomenclature : cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
GENEID : 1477
Locus Tag : RP5-1167H4.1
HGNC : 2483
MIM : 600369
Vega : OTTHUMG00000032791
Target GenBank: BC001011

 

Reference Sequence Alignment

  

HsCD00044759        ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
NM_001033522.1      ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
NM_001324.2         ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
NM_001033521.1      ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
                    ************************************************************

HsCD00044759        CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
NM_001033522.1      CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
NM_001324.2         CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
NM_001033521.1      CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
                    ************************************************************

HsCD00044759        CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
NM_001033522.1      CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
NM_001324.2         CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
NM_001033521.1      CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
                    ************************************************************

HsCD00044759        GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
NM_001033522.1      GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
NM_001324.2         GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
NM_001033521.1      GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
                    ************************************************************

HsCD00044759        ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
NM_001033522.1      ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
NM_001324.2         ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
NM_001033521.1      ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
                    ************************************************************

HsCD00044759        ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
NM_001033522.1      ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
NM_001324.2         ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
NM_001033521.1      ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
                    ************************************************************

HsCD00044759        CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
NM_001033522.1      CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
NM_001324.2         CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
NM_001033521.1      CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
                    ************************************************************

HsCD00044759        TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
NM_001033522.1      TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
NM_001324.2         TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
NM_001033521.1      TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
                    ************************************************************

HsCD00044759        GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
NM_001033522.1      GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
NM_001324.2         GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
NM_001033521.1      GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
                    ************************************************************

HsCD00044759        TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
NM_001033522.1      TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
NM_001324.2         TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
NM_001033521.1      TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
                    ************************************************************

HsCD00044759        GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
NM_001033522.1      GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
NM_001324.2         GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
NM_001033521.1      GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
                    ************************************************************

HsCD00044759        CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
NM_001033522.1      CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
NM_001324.2         CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
NM_001033521.1      CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
                    ************************************************************

HsCD00044759        CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
NM_001033522.1      CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
NM_001324.2         CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
NM_001033521.1      CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
                    ************************************************************

HsCD00044759        CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
NM_001033522.1      CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
NM_001324.2         CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
NM_001033521.1      CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
                    ************************************************************

HsCD00044759        AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
NM_001033522.1      AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
NM_001324.2         AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
NM_001033521.1      AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
                    ************************************************************

HsCD00044759        GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
NM_001033522.1      GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
NM_001324.2         GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
NM_001033521.1      GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
                    ************************************************************

HsCD00044759        ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
NM_001033522.1      ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
NM_001324.2         ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
NM_001033521.1      ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
                    ************************************************************

HsCD00044759        CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
NM_001033522.1      CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
NM_001324.2         CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
NM_001033521.1      CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
                    ************************************************************

HsCD00044759        TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
NM_001033522.1      TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
NM_001324.2         TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
NM_001033521.1      TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
                    ************************************************************

HsCD00044759        TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
NM_001033522.1      TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
NM_001324.2         TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
NM_001033521.1      TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
                    ************************************************************

HsCD00044759        CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
NM_001033522.1      CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
NM_001324.2         CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
NM_001033521.1      CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
                    ************************************************************

HsCD00044759        GCGCGGTTTTGGTACCGGAGATCGACCACTGACTAG
NM_001033522.1      GCGCGGTTTTGGTACCGGAGATCGACCACTGACTGA
NM_001324.2         GCGCGGTTTTGGTACCGGAGATCGACCACTGACTGA
NM_001033521.1      GCGCGGTTTTGGTACCGGAGATCGACCACTGACTGA
                    **********************************..

Pathways

NCI : BARD1 signaling events
Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of Intronless pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : Processing of Capped Intronless Pre-mRNA
Reactome : Processing of Intronless Pre-mRNAs
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : WD40 : WD40 repeats
UniProt : Q05048
UniProt : Q5QPD8
HPRD : 02653

Clone

Cloning Information : 188986
HIP Master Clone ID : 163930
Original Clone ID : FLH188986.01X

Organism


TAX_ID : 9606
Species Specific ID: 1477

Genome

Chromosome : 20
Map Location : 20q13.2
Ensembl : ENSG00000101138

Reagents

Labome : CSTF1-antibody