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EXOSC2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EXOSC2
Gene Name:  exosome component 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH189374.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00044805

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 882nts         Open reading frame : 1 to 882
Reference Sequence Alignment

ATGGCGATGGAGATGAGGCTTCCAGTGGCTCGCAAGCCTCTTAGCGAGAGACTGGGCCGC
GACACTAAGAAACATCTAGTGGTGCCGGGGGATACAATCACTACGGACACAGGATTCATG
CGGGGCCATGGAACGTATATGGGAGAAGAGAAGCTCATTGCATCTGTTGCTGGCTCTGTG
GAGAGAGTAAACAAGTTGATCTGTGTGAAAGCTTTGAAAACCAGATACATTGGTGAAGTA
GGAGACATCGTAGTGGGACGAATCACAGAGGTTCAACAGAAGAGGTGGAAGGTGGAGACC
AACTCCAGGCTGGATTCGGTCTTGCTGCTCTCGTCCATGAACCTTCCTGGAGGAGAGCTG
AGGAGAAGATCTGCAGAAGATGAGCTTGCAATGAGAGGTTTCTTACAGGAAGGGGACCTT
ATCAGTGCTGAGGTCCAGGCAGTGTTCTCTGACGGAGCTGTCTCTTTGCACACGAGGAGC
CTGAAATATGGAAAACTAGGTCAGGGGGTTTTGGTCCAGGTTTCCCCCTCCCTGGTGAAA
CGGCAGAAGACCCACTTTCATGATTTGCCATGTGGTGCCTCAGTGATTCTCGGTAACAAC
GGCTTCATCTGGATTTACCCAACACCTGAGCACAAAGAAGAGGAAGCAGGGGGCTTCATT
GCAAACCTGGAGCCTGTCTCTCTTGCTGATCGAGAGGTGATATCCCGGCTTCGGAACTGC
ATCATCTCGCTGGTAACTCAGAGGATGATGCTGTATGATACCAGCATCCTGTACTGCTAT
GAAGCATCCCTTCCACATCAGATCAAAGACATCTTAAAGCCAGAAATAATGGAGGAGATT
GTGATGGAAACACGCCAGAGGCTTTTGGAACAGGAGGGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 893
Start on reference sequence 12
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EXOSC2
Symbol Nomenclature : EXOSC2
SYNONYM : RRP4; Rrp4p; hRrp4p; p7
Designation : exosome complex component RRP4|exosome complex exonuclease RRP4|homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP4)|homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease|ribosomal RNA-processing protein 4
Full Nomenclature : exosome component 2
GENEID : 23404
Locus Tag : RP11-57C19.4
HGNC : 17097
MIM : 602238
Vega : OTTHUMG00000020811
Target GenBank: BC000747

 

Reference Sequence Alignment

  

HsCD00044805        ATGGCGATGGAGATGAGGCTTCCAGTGGCTCGCAAGCCTCTTAGCGAGAGACTGGGCCGC
NM_001282708.1      ATGGCGATGGAGATGAGGCTTCCAGTGGCTCGCAAGCCTCTTAGCGAGAGACTGGGCCGC
NM_014285.6         ATGGCGATGGAGATGAGGCTTCCAGTGGCTCGCAAGCCTCTTAGCGAGAGACTGGGCCGC
                    ************************************************************

HsCD00044805        GACACTAAGAAACATCTAGTGGTGCCGGGGGATACAATCACTACGGACACAGGATTCATG
NM_001282708.1      GACACTAAGAAACATCTAGTGGTGCCGGGGGATACAATCACTACGGACACAGGATTCATG
NM_014285.6         GACACTAAGAAACATCTAGTGGTGCCGGGGGATACAATCACTACGGACACAGGATTCATG
                    ************************************************************

HsCD00044805        CGGGGCCATGGAACGTATATGGGAGAAGAGAAGCTCATTGCATCTGTTGCTGGCTCTGTG
NM_001282708.1      CGGGGCCATGGAACGTATATGGGAGAAGAGAAGCTCATTGCATCTGTTGCTGGCTCTGTG
NM_014285.6         CGGGGCCATGGAACGTATATGGGAGAAGAGAAGCTCATTGCATCTGTTGCTGGCTCTGTG
                    ************************************************************

HsCD00044805        GAGAGAGTAAACAAGTTGATCTGTGTGAAAGCTTTGAAAACCAGATACATTGGTGAAGTA
NM_001282708.1      GAGAGAGTAAACAAGTTGATCTGTGTGAAAGCTTTGAAAACCAGATACATTGGTGAAGTA
NM_014285.6         GAGAGAGTAAACAAGTTGATCTGTGTGAAAGCTTTGAAAACCAGATACATTGGTGAAGTA
                    ************************************************************

HsCD00044805        GGAGACATCGTAGTGGGACGAATCACAGAGGTTCAACAGAAGAGGTGGAAGGTGGAGACC
NM_001282708.1      GGAGACATCGTAGTGGGACGAATCACAGAGGTTCAACAGAAGAGGTGGAAGGTGGAGACC
NM_014285.6         GGAGACATCGTAGTGGGACGAATCACAGAGGTTCAACAGAAGAGGTGGAAGGTGGAGACC
                    ************************************************************

HsCD00044805        AACTCCAGGCTGGATTCGGTCTTGCTGCTCTCGTCCATGAACCTTCCTGGAGGAGAGCTG
NM_001282708.1      AACTCCAGGCTGGATTCGGTCTTGCTGCTCTCGTCCATGAACCTTCCTGGAGGAGAGCTG
NM_014285.6         AACTCCAGGCTGGATTCGGTCTTGCTGCTCTCGTCCATGAACCTTCCTGGAGGAGAGCTG
                    ************************************************************

HsCD00044805        AGGAGAAGATCTGCAGAAGATGAGCTTGCAATGAGAGGTTTCTTACAGGAAGGGGACCTT
NM_001282708.1      AGGAGAAGATCTGCAGAAGATGAGCTTGCAATGAGAGGTTTCTTACAGGAAGGGGACCTT
NM_014285.6         AGGAGAAGATCTGCAGAAGATGAGCTTGCAATGAGAGGTTTCTTACAGGAAGGGGACCTT
                    ************************************************************

HsCD00044805        ATCAGTGCTGAGGTCCAGGCAGTGTTCTCTGACGGAGCTGTCTCTTTGCACACGAGGAGC
NM_001282708.1      ATCAGTGGG---------------------------------------------------
NM_014285.6         ATCAGTGCTGAGGTCCAGGCAGTGTTCTCTGACGGAGCTGTCTCTTTGCACACGAGGAGC
                    *******                                                     

HsCD00044805        CTGAAATATGGAAAACTAGGTCAGGGGGTTTTGGTCCAGGTTTCCCCCTCCCTGGTGAAA
NM_001282708.1      ---------------------------GTTTTGGTCCAGGTTTCCCCCTCCCTGGTGAAA
NM_014285.6         CTGAAATATGGAAAACTAGGTCAGGGGGTTTTGGTCCAGGTTTCCCCCTCCCTGGTGAAA
                                               *********************************

HsCD00044805        CGGCAGAAGACCCACTTTCATGATTTGCCATGTGGTGCCTCAGTGATTCTCGGTAACAAC
NM_001282708.1      CGGCAGAAGACCCACTTTCATGATTTGCCATGTGGTGCCTCAGTGATTCTCGGTAACAAC
NM_014285.6         CGGCAGAAGACCCACTTTCATGATTTGCCATGTGGTGCCTCAGTGATTCTCGGTAACAAC
                    ************************************************************

HsCD00044805        GGCTTCATCTGGATTTACCCAACACCTGAGCACAAAGAAGAGGAAGCAGGGGGCTTCATT
NM_001282708.1      GGCTTCATCTGGATTTACCCAACACCTGAGCACAAAGAAGAGGAAGCAGGGGGCTTCATT
NM_014285.6         GGCTTCATCTGGATTTACCCAACACCTGAGCACAAAGAAGAGGAAGCAGGGGGCTTCATT
                    ************************************************************

HsCD00044805        GCAAACCTGGAGCCTGTCTCTCTTGCTGATCGAGAGGTGATATCCCGGCTTCGGAACTGC
NM_001282708.1      GCAAACCTGGAGCCTGTCTCTCTTGCTGATCGAGAGGTGATATCCCGGCTTCGGAACTGC
NM_014285.6         GCAAACCTGGAGCCTGTCTCTCTTGCTGATCGAGAGGTGATATCCCGGCTTCGGAACTGC
                    ************************************************************

HsCD00044805        ATCATCTCGCTGGTAACTCAGAGGATGATGCTGTATGATACCAGCATCCTGTACTGCTAT
NM_001282708.1      ATCATCTCGCTGGTAACTCAGAGGATGATGCTGTATGATACCAGCATCCTGTACTGCTAT
NM_014285.6         ATCATCTCGCTGGTAACTCAGAGGATGATGCTGTATGATACCAGCATCCTGTACTGCTAT
                    ************************************************************

HsCD00044805        GAAGCATCCCTTCCACATCAGATCAAAGACATCTTAAAGCCAGAAATAATGGAGGAGATT
NM_001282708.1      GAAGCATCCCTTCCACATCAGATCAAAGACATCTTAAAGCCAGAAATAATGGAGGAGATT
NM_014285.6         GAAGCATCCCTTCCACATCAGATCAAAGACATCTTAAAGCCAGAAATAATGGAGGAGATT
                    ************************************************************

HsCD00044805        GTGATGGAAACACGCCAGAGGCTTTTGGAACAGGAGGGATAG
NM_001282708.1      GTGATGGAAACACGCCAGAGGCTTTTGGAACAGGAGGGATAA
NM_014285.6         GTGATGGAAACACGCCAGAGGCTTTTGGAACAGGAGGGATAA
                    *****************************************.

Pathways

Reactome : ATF4 activates genes
Reactome : Butyrate Response Factor 1 (BRF1) destabilizes mRNA
Reactome : Deadenylation-dependent mRNA decay
Reactome : Gene Expression
Reactome : KSRP destabilizes mRNA
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Metabolism of proteins
Reactome : PERK regulates gene expression
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome : Tristetraprolin (TTP) destabilizes mRNA
Reactome : Unfolded Protein Response (UPR)
Reactome : mRNA decay by 3' to 5' exoribonuclease

Protein

UniProt : Q13868
HPRD : 03758

Clone

Cloning Information : 189374
HIP Master Clone ID : 164318
Original Clone ID : FLH189374.01X

Organism


TAX_ID : 9606
Species Specific ID: 23404

Genome

Chromosome : 9
Map Location : 9q34
Ensembl : ENSG00000130713

Reagents

Labome : EXOSC2-antibody