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Detailed Vector Information: pMCSG69

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG69              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with a T7 promoter and N-terminal MBP tag, TVMV cleavage site, 6xHis tag and TEV protease cleavage site with rare codon argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00450865

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG69

pMCSG69 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and N-terminal MBP tag, TVMV cleavage site, 6xHis tag and TEV protease cleavage site with rare codon argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region plus a portion of the beta-lactamase gene from pMCSG19 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG19 with truncated ori and the argU & ileX tRNA genes
Size (bp): 58963
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR332 origin of replication 3956 4544
ligation independent cloning LIC ligation independent cloning site 204 235
promoter T7 T7 promoter 1515 1531
protease cleavage site TEV TEV protease cleavage site 223 243
protease cleavage site TVMV TVMV cleavage site 286 306
repressor protein gene LacI LacI gene 2568 3527
selectable marker AmpR ampicillin resistance gene 4715 5575
tRNA argU argU tRNA gene 1598 1954
tRNA ileX ileX tRNA gene 2114 2265
tag MBP N-terminal maltose binding protein (MBP) tag 337 1443
tag His N-terminal 6xHis tag 268 285
trxn termination sequence T7 term T7 termination sequence 26 72

 

Species Specific ID: None
Target GenBank: None