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PACSIN3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PACSIN3
Gene Name:  protein kinase C and casein kinase substrate in neurons 3
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH194426.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 24500282
Title: Syndapin 3 Modulates Fusion Pore Expansion in Mouse Neuroendocrine Chromaffin Cells
Authors: HIP
HsCD00045184

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1275nts         Open reading frame : 1 to 1275
Reference Sequence Alignment

ATGGCTCCAGAAGAGGACGCTGGAGGGGAGGCCTTAGGGGGCAGTTTCTGGGAGGCTGGC
AACTACAGGCGCACGGTACAGCGGGTGGAGGACGGGCACCGGCTGTGCGGGGACCTGGTC
AGCTGCTTCCAGGAGCGCGCCCGCATCGAGAAGGCTTATGCCCAGCAGTTGGCTGACTGG
GCCCGAAAGTGGAGGGGGACCGTGGAGAAGGGCCCCCAGTATGGCACACTGGAGAAGGCC
TGGCATGCCTTTTTCACGGCGGCTGAGCGGCTGAGCGCGCTGCACCTGGAGGTGCGGGAG
AAGCTGCAAGGGCAGGACAGTGAGCGGGTGCGCGCCTGGCAGCGGGGGGCTTTCCACCGG
CCTGTGCTGGGCGGCTTCCGCGAGAGCCGGGCGGCCGAGGACGGCTTCCGCAAGGCCCAG
AAGCCCTGGCTGAAGAGGCTGAAGGAGGTTGAGGCTTCCAAGAAAAGCTACCACGCAGCC
CGGAAGGATGAGAAGACCGCCCAGACGAGGGAGAGCCACGCAAAGGCAGACAGCGCCGTC
TCCCAGGAGCAGCTGCGCAAACTGCAGGAACGGGTGGAACGCTGTGCCAAGGAGGCCGAG
AAGACAAAAGCTCAGTATGAGCAGACGCTGGCAGAGCTGCATCGCTACACTCCACGCTAC
ATGGAGGACATGGAACAGGCCTTTGAGACCTGCCAGGCCGCCGAGCGCCAGCGGCTTCTT
TTCTTCAAGGATATGCTGCTCACCTTACACCAGCACCTGGACCTTTCCAGCAGTGAGAAG
TTCCATGAACTCCACCGTGACTTGCACCAGGGCATTGAGGCAGCCAGTGACGAAGAGGAT
CTGCGCTGGTGGCGCAGCACCCACGGGCCAGGCATGGCCATGAACTGGCCACAGTTCGAG
GAGTGGTCCTTGGACACACAGAGGACAATCAGCCGGAAAGAGAAGGGTGGCCGGAGCCCT
GATGAGGTTACCCTGACCAGCATTGTGCCTACAAGAGATGGCACCGCACCCCCACCCCAG
TCCCCGGGGTCCCCAGGCACGGGGCAGGATGAGGAGTGGTCAGATGAAGAGAGTCCCCGG
AAGGCTGCCACCGGGGTTCGGGTGAGGGCACTCTATGACTACGCTGGCCAGGAAGCTGAT
GAGCTGAGCTTCCGAGCAGGGGAGGAGCTGCTGAAGATGAGTGAGGAGGACGAGCAGGGC
TGGTGCCAAGGCCAGTTGCAGAGTGGCCGCATTGGCCTGTACCCTGCCAACTACGTGGAG
TGTGTGGGCGCCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1427
Start on reference sequence 153
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PACSIN3
Symbol Nomenclature : PACSIN3
SYNONYM : SDPIII
Designation : SH3 domain-containing protein 6511|protein kinase C and casein kinase substrate in neurons protein 3|syndapin III
Full Nomenclature : protein kinase C and casein kinase substrate in neurons 3
GENEID : 29763
HGNC : 8572
MIM : 606513
Vega : OTTHUMG00000166870
Target GenBank: BC007914

 

Reference Sequence Alignment

  

HsCD00045184        ATGGCTCCAGAAGAGGACGCTGGAGGGGAGGCCTTAGGGGGCAGTTTCTGGGAGGCTGGC
NM_016223.4         ATGGCTCCAGAAGAGGACGCTGGAGGGGAGGCCTTAGGGGGCAGTTTCTGGGAGGCTGGC
NM_001184975.1      ATGGCTCCAGAAGAGGACGCTGGAGGGGAGGCCTTAGGGGGCAGTTTCTGGGAGGCTGGC
NM_001184974.1      ATGGCTCCAGAAGAGGACGCTGGAGGGGAGGCCTTAGGGGGCAGTTTCTGGGAGGCTGGC
                    ************************************************************

HsCD00045184        AACTACAGGCGCACGGTACAGCGGGTGGAGGACGGGCACCGGCTGTGCGGGGACCTGGTC
NM_016223.4         AACTACAGGCGCACGGTACAGCGGGTGGAGGACGGGCACCGGCTGTGCGGGGACCTGGTC
NM_001184975.1      AACTACAGGCGCACGGTACAGCGGGTGGAGGACGGGCACCGGCTGTGCGGGGACCTGGTC
NM_001184974.1      AACTACAGGCGCACGGTACAGCGGGTGGAGGACGGGCACCGGCTGTGCGGGGACCTGGTC
                    ************************************************************

HsCD00045184        AGCTGCTTCCAGGAGCGCGCCCGCATCGAGAAGGCTTATGCCCAGCAGTTGGCTGACTGG
NM_016223.4         AGCTGCTTCCAGGAGCGCGCCCGCATCGAGAAGGCTTATGCCCAGCAGTTGGCTGACTGG
NM_001184975.1      AGCTGCTTCCAGGAGCGCGCCCGCATCGAGAAGGCTTATGCCCAGCAGTTGGCTGACTGG
NM_001184974.1      AGCTGCTTCCAGGAGCGCGCCCGCATCGAGAAGGCTTATGCCCAGCAGTTGGCTGACTGG
                    ************************************************************

HsCD00045184        GCCCGAAAGTGGAGGGGGACCGTGGAGAAGGGCCCCCAGTATGGCACACTGGAGAAGGCC
NM_016223.4         GCCCGAAAGTGGAGGGGGACCGTGGAGAAGGGCCCCCAGTATGGCACACTGGAGAAGGCC
NM_001184975.1      GCCCGAAAGTGGAGGGGGACCGTGGAGAAGGGCCCCCAGTATGGCACACTGGAGAAGGCC
NM_001184974.1      GCCCGAAAGTGGAGGGGGACCGTGGAGAAGGGCCCCCAGTATGGCACACTGGAGAAGGCC
                    ************************************************************

HsCD00045184        TGGCATGCCTTTTTCACGGCGGCTGAGCGGCTGAGCGCGCTGCACCTGGAGGTGCGGGAG
NM_016223.4         TGGCATGCCTTTTTCACGGCGGCTGAGCGGCTGAGCGCGCTGCACCTGGAGGTGCGGGAG
NM_001184975.1      TGGCATGCCTTTTTCACGGCGGCTGAGCGGCTGAGCGCGCTGCACCTGGAGGTGCGGGAG
NM_001184974.1      TGGCATGCCTTTTTCACGGCGGCTGAGCGGCTGAGCGCGCTGCACCTGGAGGTGCGGGAG
                    ************************************************************

HsCD00045184        AAGCTGCAAGGGCAGGACAGTGAGCGGGTGCGCGCCTGGCAGCGGGGGGCTTTCCACCGG
NM_016223.4         AAGCTGCAAGGGCAGGACAGTGAGCGGGTGCGCGCCTGGCAGCGGGGGGCTTTCCACCGG
NM_001184975.1      AAGCTGCAAGGGCAGGACAGTGAGCGGGTGCGCGCCTGGCAGCGGGGGGCTTTCCACCGG
NM_001184974.1      AAGCTGCAAGGGCAGGACAGTGAGCGGGTGCGCGCCTGGCAGCGGGGGGCTTTCCACCGG
                    ************************************************************

HsCD00045184        CCTGTGCTGGGCGGCTTCCGCGAGAGCCGGGCGGCCGAGGACGGCTTCCGCAAGGCCCAG
NM_016223.4         CCTGTGCTGGGCGGCTTCCGCGAGAGCCGGGCGGCCGAGGACGGCTTCCGCAAGGCCCAG
NM_001184975.1      CCTGTGCTGGGCGGCTTCCGCGAGAGCCGGGCGGCCGAGGACGGCTTCCGCAAGGCCCAG
NM_001184974.1      CCTGTGCTGGGCGGCTTCCGCGAGAGCCGGGCGGCCGAGGACGGCTTCCGCAAGGCCCAG
                    ************************************************************

HsCD00045184        AAGCCCTGGCTGAAGAGGCTGAAGGAGGTTGAGGCTTCCAAGAAAAGCTACCACGCAGCC
NM_016223.4         AAGCCCTGGCTGAAGAGGCTGAAGGAGGTTGAGGCTTCCAAGAAAAGCTACCACGCAGCC
NM_001184975.1      AAGCCCTGGCTGAAGAGGCTGAAGGAGGTTGAGGCTTCCAAGAAAAGCTACCACGCAGCC
NM_001184974.1      AAGCCCTGGCTGAAGAGGCTGAAGGAGGTTGAGGCTTCCAAGAAAAGCTACCACGCAGCC
                    ************************************************************

HsCD00045184        CGGAAGGATGAGAAGACCGCCCAGACGAGGGAGAGCCACGCAAAGGCAGACAGCGCCGTC
NM_016223.4         CGGAAGGATGAGAAGACCGCCCAGACGAGGGAGAGCCACGCAAAGGCAGACAGCGCCGTC
NM_001184975.1      CGGAAGGATGAGAAGACCGCCCAGACGAGGGAGAGCCACGCAAAGGCAGACAGCGCCGTC
NM_001184974.1      CGGAAGGATGAGAAGACCGCCCAGACGAGGGAGAGCCACGCAAAGGCAGACAGCGCCGTC
                    ************************************************************

HsCD00045184        TCCCAGGAGCAGCTGCGCAAACTGCAGGAACGGGTGGAACGCTGTGCCAAGGAGGCCGAG
NM_016223.4         TCCCAGGAGCAGCTGCGCAAACTGCAGGAACGGGTGGAACGCTGTGCCAAGGAGGCCGAG
NM_001184975.1      TCCCAGGAGCAGCTGCGCAAACTGCAGGAACGGGTGGAACGCTGTGCCAAGGAGGCCGAG
NM_001184974.1      TCCCAGGAGCAGCTGCGCAAACTGCAGGAACGGGTGGAACGCTGTGCCAAGGAGGCCGAG
                    ************************************************************

HsCD00045184        AAGACAAAAGCTCAGTATGAGCAGACGCTGGCAGAGCTGCATCGCTACACTCCACGCTAC
NM_016223.4         AAGACAAAAGCTCAGTATGAGCAGACGCTGGCAGAGCTGCATCGCTACACTCCACGCTAC
NM_001184975.1      AAGACAAAAGCTCAGTATGAGCAGACGCTGGCAGAGCTGCATCGCTACACTCCACGCTAC
NM_001184974.1      AAGACAAAAGCTCAGTATGAGCAGACGCTGGCAGAGCTGCATCGCTACACTCCACGCTAC
                    ************************************************************

HsCD00045184        ATGGAGGACATGGAACAGGCCTTTGAGACCTGCCAGGCCGCCGAGCGCCAGCGGCTTCTT
NM_016223.4         ATGGAGGACATGGAACAGGCCTTTGAGACCTGCCAGGCCGCCGAGCGCCAGCGGCTTCTT
NM_001184975.1      ATGGAGGACATGGAACAGGCCTTTGAGACCTGCCAGGCCGCCGAGCGCCAGCGGCTTCTT
NM_001184974.1      ATGGAGGACATGGAACAGGCCTTTGAGACCTGCCAGGCCGCCGAGCGCCAGCGGCTTCTT
                    ************************************************************

HsCD00045184        TTCTTCAAGGATATGCTGCTCACCTTACACCAGCACCTGGACCTTTCCAGCAGTGAGAAG
NM_016223.4         TTCTTCAAGGATATGCTGCTCACCTTACACCAGCACCTGGACCTTTCCAGCAGTGAGAAG
NM_001184975.1      TTCTTCAAGGATATGCTGCTCACCTTACACCAGCACCTGGACCTTTCCAGCAGTGAGAAG
NM_001184974.1      TTCTTCAAGGATATGCTGCTCACCTTACACCAGCACCTGGACCTTTCCAGCAGTGAGAAG
                    ************************************************************

HsCD00045184        TTCCATGAACTCCACCGTGACTTGCACCAGGGCATTGAGGCAGCCAGTGACGAAGAGGAT
NM_016223.4         TTCCATGAACTCCACCGTGACTTGCACCAGGGCATTGAGGCAGCCAGTGACGAAGAGGAT
NM_001184975.1      TTCCATGAACTCCACCGTGACTTGCACCAGGGCATTGAGGCAGCCAGTGACGAAGAGGAT
NM_001184974.1      TTCCATGAACTCCACCGTGACTTGCACCAGGGCATTGAGGCAGCCAGTGACGAAGAGGAT
                    ************************************************************

HsCD00045184        CTGCGCTGGTGGCGCAGCACCCACGGGCCAGGCATGGCCATGAACTGGCCACAGTTCGAG
NM_016223.4         CTGCGCTGGTGGCGCAGCACCCACGGGCCAGGCATGGCCATGAACTGGCCACAGTTCGAG
NM_001184975.1      CTGCGCTGGTGGCGCAGCACCCACGGGCCAGGCATGGCCATGAACTGGCCACAGTTCGAG
NM_001184974.1      CTGCGCTGGTGGCGCAGCACCCACGGGCCAGGCATGGCCATGAACTGGCCACAGTTCGAG
                    ************************************************************

HsCD00045184        GAGTGGTCCTTGGACACACAGAGGACAATCAGCCGGAAAGAGAAGGGTGGCCGGAGCCCT
NM_016223.4         GAGTGGTCCTTGGACACACAGAGGACAATCAGCCGGAAAGAGAAGGGTGGCCGGAGCCCT
NM_001184975.1      GAGTGGTCCTTGGACACACAGAGGACAATCAGCCGGAAAGAGAAGGGTGGCCGGAGCCCT
NM_001184974.1      GAGTGGTCCTTGGACACACAGAGGACAATCAGCCGGAAAGAGAAGGGTGGCCGGAGCCCT
                    ************************************************************

HsCD00045184        GATGAGGTTACCCTGACCAGCATTGTGCCTACAAGAGATGGCACCGCACCCCCACCCCAG
NM_016223.4         GATGAGGTTACCCTGACCAGCATTGTGCCTACAAGAGATGGCACCGCACCCCCACCCCAG
NM_001184975.1      GATGAGGTTACCCTGACCAGCATTGTGCCTACAAGAGATGGCACCGCACCCCCACCCCAG
NM_001184974.1      GATGAGGTTACCCTGACCAGCATTGTGCCTACAAGAGATGGCACCGCACCCCCACCCCAG
                    ************************************************************

HsCD00045184        TCCCCGGGGTCCCCAGGCACGGGGCAGGATGAGGAGTGGTCAGATGAAGAGAGTCCCCGG
NM_016223.4         TCCCCGGGGTCCCCAGGCACGGGGCAGGATGAGGAGTGGTCAGATGAAGAGAGTCCCCGG
NM_001184975.1      TCCCCGGGGTCCCCAGGCACGGGGCAGGATGAGGAGTGGTCAGATGAAGAGAGTCCCCGG
NM_001184974.1      TCCCCGGGGTCCCCAGGCACGGGGCAGGATGAGGAGTGGTCAGATGAAGAGAGTCCCCGG
                    ************************************************************

HsCD00045184        AAGGCTGCCACCGGGGTTCGGGTGAGGGCACTCTATGACTACGCTGGCCAGGAAGCTGAT
NM_016223.4         AAGGCTGCCACCGGGGTTCGGGTGAGGGCACTCTATGACTACGCTGGCCAGGAAGCTGAT
NM_001184975.1      AAGGCTGCCACCGGGGTTCGGGTGAGGGCACTCTATGACTACGCTGGCCAGGAAGCTGAT
NM_001184974.1      AAGGCTGCCACCGGGGTTCGGGTGAGGGCACTCTATGACTACGCTGGCCAGGAAGCTGAT
                    ************************************************************

HsCD00045184        GAGCTGAGCTTCCGAGCAGGGGAGGAGCTGCTGAAGATGAGTGAGGAGGACGAGCAGGGC
NM_016223.4         GAGCTGAGCTTCCGAGCAGGGGAGGAGCTGCTGAAGATGAGTGAGGAGGACGAGCAGGGC
NM_001184975.1      GAGCTGAGCTTCCGAGCAGGGGAGGAGCTGCTGAAGATGAGTGAGGAGGACGAGCAGGGC
NM_001184974.1      GAGCTGAGCTTCCGAGCAGGGGAGGAGCTGCTGAAGATGAGTGAGGAGGACGAGCAGGGC
                    ************************************************************

HsCD00045184        TGGTGCCAAGGCCAGTTGCAGAGTGGCCGCATTGGCCTGTACCCTGCCAACTACGTGGAG
NM_016223.4         TGGTGCCAAGGCCAGTTGCAGAGTGGCCGCATTGGCCTGTACCCTGCCAACTACGTGGAG
NM_001184975.1      TGGTGCCAAGGCCAGTTGCAGAGTGGCCGCATTGGCCTGTACCCTGCCAACTACGTGGAG
NM_001184974.1      TGGTGCCAAGGCCAGTTGCAGAGTGGCCGCATTGGCCTGTACCCTGCCAACTACGTGGAG
                    ************************************************************

HsCD00045184        TGTGTGGGCGCCTAG
NM_016223.4         TGTGTGGGCGCCTGA
NM_001184975.1      TGTGTGGGCGCCTGA
NM_001184974.1      TGTGTGGGCGCCTGA
                    *************..

Protein

SMART domain : SH3 : Src homology 3 domains
SMART domain : FCH : Fes/CIP4 homology domain
UniProt : Q9UKS6
HPRD : 05937

Clone

Cloning Information : 194426
HIP Master Clone ID : 169137
Original Clone ID : FLH194426.01X

Organism


TAX_ID : 9606
Species Specific ID: 29763

Genome

Chromosome : 11
Map Location : 11p12-p11.12
Ensembl : ENSG00000165912

Reagents

Labome : PACSIN3-antibody