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ZNF259 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF259
Gene Name:  zinc finger protein 259
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH026823.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00004520

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1380nts         Open reading frame : 1 to 1380
Reference Sequence Alignment

ATGGCGGCCAGCGGGGCTGTGGAACCAGGGCCCCCGGGGGCTGCCGTCGCCCCGTCGCCC
GCCCCGGCCCCGCCGCCTGCCCCTGATCACCTGTTCCGGCCCATCAGCGCCGAGGACGAG
GAGCAGCAGCCCACCGAGATCGAGTCGCTATGCATGAACTGTTACTGCAATGGCATGACG
CGCCTCCTGCTCACCAAGATTCCCTTCTTCAGAGAAATAATAGTGAGCTCCTTTTCCTGC
GAGCACTGTGGCTGGAACAACACGGAGATCCAGTCGGCAGGCAGGATCCAGGACCAGGGA
GTGCGCTACACTTTGTCTGTCAGGGCTCTGGAGGACATGAACAGAGAAGTGGTGAAGACT
GACTCTGCTGCCACAAGGATTCCTGAGCTAGATTTTGAAATTCCTGCCTTTAGCCAGAAA
GGAGCTCTGACCACTGTTGAAGGATTGATCACCCGTGCTATCTCTGGCCTGGAGCAGGAC
CAGCCTGCACGAAGGGCAAACAAAGATGCTACAGCTGAAAGAATTGATGAGTTCATTGTC
AAACTGAAGGAGCTAAAGCAAGTAGCCTCCCCTTTCACTCTGATCATTGATGATCCCTCA
GGGAACAGTTTTGTGGAAAACCCACATGCTCCTCAGAAAGATGATGCCCTGGTGATCACA
CACTACAACCGGACCCGACAGCAGGAAGAGATGCTGGGGCTTCAAGAAGAAGCACCAGCA
GAGAAGCCAGAAGAGGAAGATCTCAGAAATGAAGTGCTCCAGTTCAGCACAAACTGCCCA
GAATGCAATGCCCCCGCTCAGACCAACATGAAGCTAGTACAAATCCCTCACTTTAAGGAG
GTTATCATCATGGCTACCAACTGCGAGAACTGTGGGCATCGGACCAATGAGGTGAAATCT
GGAGGAGCAGTAGAACCCTTGGGCACCAGGATCACCCTCCACATCACAGATGCCTCAGAT
ATGACCAGAGACCTCCTCAAGTCTGAGACTTGCAGTGTGGAAATCCCAGAGCTAGAATTT
GAACTGGGAATGGCAGTCCTCGGGGGCAAGTTCACCACACTGGAAGGGCTGCTGAAAGAC
ATCCGGGAACTGGTGACCAAAAATCCTTTCACACTGGGCGACAGTTCCAATCCTGGACAG
ACGGAGAGACTACAGGAGTTTAGCCAGAAGATGGACCAGATCATCGAAGGTAACATGAAG
GCCCACTTTATTATGGATGATCCAGCAGGAAACAGTTACTTGCAGAATGTGTATGCGCCT
GAAGATGATCCTGAGATGAAGGTGGAGCGTTACAAGCGCACCTTTGACCAAAATGAGGAG
CTAGGGCTCAATGACATGAAGACAGAGGGCTATGAGGCAGGCCTGGCTCCGCAACGGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1405
Start on reference sequence 26
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF259
Symbol Nomenclature : ZNF259
SYNONYM : ZPR1
Designation : zinc finger protein ZPR1
Full Nomenclature : zinc finger protein 259
GENEID : 8882
GI : 61361933
GenBank Accession : AY890187
HGNC : 13051
MIM : 603901
Target GenBank: BC017380

 

Reference Sequence Alignment

  

HsCD00004520      ATGGCGGCCAGCGGGGCTGTGGAACCAGGGCCCCCGGGGGCTGCCGTCGCCCCGTCGCCC
NM_003904.3       ATGGCGGCCAGCGGGGCTGTGGAACCAGGGCCCCCGGGGGCTGCCGTCGCCCCGTCGCCC
                  ************************************************************

HsCD00004520      GCCCCGGCCCCGCCGCCTGCCCCTGATCACCTGTTCCGGCCCATCAGCGCCGAGGACGAG
NM_003904.3       GCCCCGGCCCCGCCGCCTGCCCCTGATCACCTGTTCCGGCCCATCAGCGCCGAGGACGAG
                  ************************************************************

HsCD00004520      GAGCAGCAGCCCACCGAGATCGAGTCGCTATGCATGAACTGTTACTGCAATGGCATGACG
NM_003904.3       GAGCAGCAGCCCACCGAGATCGAGTCGCTATGCATGAACTGTTACTGCAATGGCATGACG
                  ************************************************************

HsCD00004520      CGCCTCCTGCTCACCAAGATTCCCTTCTTCAGAGAAATAATAGTGAGCTCCTTTTCCTGC
NM_003904.3       CGCCTCCTGCTCACCAAGATTCCCTTCTTCAGAGAAATAATAGTGAGCTCCTTTTCCTGC
                  ************************************************************

HsCD00004520      GAGCACTGTGGCTGGAACAACACGGAGATCCAGTCGGCAGGCAGGATCCAGGACCAGGGA
NM_003904.3       GAGCACTGTGGCTGGAACAACACGGAGATCCAGTCGGCAGGCAGGATCCAGGACCAGGGA
                  ************************************************************

HsCD00004520      GTGCGCTACACTTTGTCTGTCAGGGCTCTGGAGGACATGAACAGAGAAGTGGTGAAGACT
NM_003904.3       GTGCGCTACACTTTGTCTGTCAGGGCTCTGGAGGACATGAACAGAGAAGTGGTGAAGACT
                  ************************************************************

HsCD00004520      GACTCTGCTGCCACAAGGATTCCTGAGCTAGATTTTGAAATTCCTGCCTTTAGCCAGAAA
NM_003904.3       GACTCTGCTGCCACAAGGATTCCTGAGCTAGATTTTGAAATTCCTGCCTTTAGCCAGAAA
                  ************************************************************

HsCD00004520      GGAGCTCTGACCACTGTTGAAGGATTGATCACCCGTGCTATCTCTGGCCTGGAGCAGGAC
NM_003904.3       GGAGCTCTGACCACTGTTGAAGGATTGATCACCCGTGCTATCTCTGGCCTGGAGCAGGAC
                  ************************************************************

HsCD00004520      CAGCCTGCACGAAGGGCAAACAAAGATGCTACAGCTGAAAGAATTGATGAGTTCATTGTC
NM_003904.3       CAGCCTGCACGAAGGGCAAACAAAGATGCTACAGCTGAAAGAATTGATGAGTTCATTGTC
                  ************************************************************

HsCD00004520      AAACTGAAGGAGCTAAAGCAAGTAGCCTCCCCTTTCACTCTGATCATTGATGATCCCTCA
NM_003904.3       AAACTGAAGGAGCTAAAGCAAGTAGCCTCCCCTTTCACTCTGATCATTGATGATCCCTCA
                  ************************************************************

HsCD00004520      GGGAACAGTTTTGTGGAAAACCCACATGCTCCTCAGAAAGATGATGCCCTGGTGATCACA
NM_003904.3       GGGAACAGTTTTGTGGAAAACCCACATGCTCCTCAGAAAGATGATGCCCTGGTGATCACA
                  ************************************************************

HsCD00004520      CACTACAACCGGACCCGACAGCAGGAAGAGATGCTGGGGCTTCAAGAAGAAGCACCAGCA
NM_003904.3       CACTACAACCGGACCCGACAGCAGGAAGAGATGCTGGGGCTTCAAGAAGAAGCACCAGCA
                  ************************************************************

HsCD00004520      GAGAAGCCAGAAGAGGAAGATCTCAGAAATGAAGTGCTCCAGTTCAGCACAAACTGCCCA
NM_003904.3       GAGAAGCCAGAAGAGGAAGATCTCAGAAATGAAGTGCTCCAGTTCAGCACAAACTGCCCA
                  ************************************************************

HsCD00004520      GAATGCAATGCCCCCGCTCAGACCAACATGAAGCTAGTACAAATCCCTCACTTTAAGGAG
NM_003904.3       GAATGCAATGCCCCCGCTCAGACCAACATGAAGCTAGTACAAATCCCTCACTTTAAGGAG
                  ************************************************************

HsCD00004520      GTTATCATCATGGCTACCAACTGCGAGAACTGTGGGCATCGGACCAATGAGGTGAAATCT
NM_003904.3       GTTATCATCATGGCTACCAACTGCGAGAACTGTGGGCATCGGACCAATGAGGTGAAATCT
                  ************************************************************

HsCD00004520      GGAGGAGCAGTAGAACCCTTGGGCACCAGGATCACCCTCCACATCACAGATGCCTCAGAT
NM_003904.3       GGAGGAGCAGTAGAACCCTTGGGCACCAGGATCACCCTCCACATCACAGATGCCTCAGAT
                  ************************************************************

HsCD00004520      ATGACCAGAGACCTCCTCAAGTCTGAGACTTGCAGTGTGGAAATCCCAGAGCTAGAATTT
NM_003904.3       ATGACCAGAGACCTCCTCAAGTCTGAGACTTGCAGTGTGGAAATCCCAGAGCTAGAATTT
                  ************************************************************

HsCD00004520      GAACTGGGAATGGCAGTCCTCGGGGGCAAGTTCACCACACTGGAAGGGCTGCTGAAAGAC
NM_003904.3       GAACTGGGAATGGCAGTCCTCGGGGGCAAGTTCACCACACTGGAAGGGCTGCTGAAAGAC
                  ************************************************************

HsCD00004520      ATCCGGGAACTGGTGACCAAAAATCCTTTCACACTGGGCGACAGTTCCAATCCTGGACAG
NM_003904.3       ATCCGGGAACTGGTGACCAAAAATCCTTTCACACTGGGCGACAGTTCCAATCCTGGACAG
                  ************************************************************

HsCD00004520      ACGGAGAGACTACAGGAGTTTAGCCAGAAGATGGACCAGATCATCGAAGGTAACATGAAG
NM_003904.3       ACGGAGAGACTACAGGAGTTTAGCCAGAAGATGGACCAGATCATCGAAGGTAACATGAAG
                  ************************************************************

HsCD00004520      GCCCACTTTATTATGGATGATCCAGCAGGAAACAGTTACTTGCAGAATGTGTATGCGCCT
NM_003904.3       GCCCACTTTATTATGGATGATCCAGCAGGAAACAGTTACTTGCAGAATGTGTATGCGCCT
                  ************************************************************

HsCD00004520      GAAGATGATCCTGAGATGAAGGTGGAGCGTTACAAGCGCACCTTTGACCAAAATGAGGAG
NM_003904.3       GAAGATGATCCTGAGATGAAGGTGGAGCGTTACAAGCGCACCTTTGACCAAAATGAGGAG
                  ************************************************************

HsCD00004520      CTAGGGCTCAATGACATGAAGACAGAGGGCTATGAGGCAGGCCTGGCTCCGCAACGGTAG
NM_003904.3       CTAGGGCTCAATGACATGAAGACAGAGGGCTATGAGGCAGGCCTGGCTCCGCAACGGTAG
                  ************************************************************

Clone

Cloning Information : 26823
HIP Master Clone ID : 57199
Original Clone ID : FLH026823.01X

Organism


TAX_ID : 9606
Species Specific ID: 8882

Genome

Chromosome : 11
Map Location : 11q23.3

Reagents

Labome : ZPR1-antibody