PFL_4533 (P. protegens Pf-5 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  bdlA
Gene Name:  biofilm dispersion protein BdlA
Original Clone ID:
APC67807.0_SG330_pMCSG7
Keyword:
full length
Species:
Pseudomonas protegens Pf-5
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
PfCD00452494
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1320nts
Open reading frame: 48 to 1367

CCGGCGAAATCTCGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCTATTCAACC
GCCACAACATTATCACCGATTCCCTGCAGCAAACCATTGCCGAACAATCCCGACTGCTGG
AAGCCATCGATCGCTCCATGGCGGTGATCGAATTCGACCTGCAGGGCAACGTCCTGCGAG
CCAACGCCAACTTTCTCCAGACCCTGGGCTATCGCGAAGACCAGGTGACAGGCCAGCCGC
ACCGCCTCTTCTGCACCCCGGAGTACGTTCGCAGCAGCGACTACCAGCAATTGTGGAACC
GCCTCAGAAGCGGCCAGTTCGAATCCGGAACCTTCGAACGGGTGGCCAGCAACGGCCAGA
GCCTGTGGCTGGAAGCCAGCTACAACCCCATCCGCGACGCATCCGGCGCAGTGTACAAAG
TGGTCAAGTACGCCCTGGATGTCACCGCCCGAGTGCAGGAAGAGGTGGAGGCACGAAACA
AGCTGGACGCCATCGACCGGGCCATGGCGGTGATCGAGTTCAACCTCGACGGCAGCATCA
TCGGTGCCAACAGCAATTTCCTCAGCCGCATGGGCTACAGCCTGGCCGAGATCAAGGGCA
AGCATCACCGCATGTTCTGCAAACCCGAGCTGATCAACAGCAGCGCCTACCAGGACTTCT
GGAACCGCCTGAACCGGGGTGAGTTCTTCAACGGTCAGTTCGAGCGGATCGACAGGCACG
GACGTACGCTGTGGCTGGAAGCCAACTACAACCCGGTCTACGACGCCAGCGGGCGCCTGT
GCAAAGTGGTCAAGTACGCCGCCGACGTCACCCAGCAGGTGGAAAAACACGAACAGGATG
CCCAGAGCGCGAGCCAGGCTTATCACATCTCGGTACAGACCCGAAAAGTCGCCGAACAAG
GCACCGGAGTGATCCAGCAAGCCGCCAGCGAAATGCGCCAGATCGCCGAGAACATCGAGG
GCTCTTCATCGCTGATCGCGCGCCTGGGCGAACGCTCCGAACAGATCACCGCCATCGTCA
ACACCATCCGCGCCATTGCCGACCAGACCAACCTGCTGGCTCTGAACGCCGCCATCGAAG
CGGCCCGGGCCGGGGACCAGGGTCGTGGTTTTGCCGTGGTGGCCGATGAAGTCCGGCAAT
TGGCGGCGCGCACCAGCGGCTCGACGGCGGAAATCTCCAGCATGATCGACATGATCCAGA
ACGAAACGCAGCAAGCCATCAAGAGCATGGACGGTACCCGGGAGCGGGCGGCCAAGGGCG
TGCAACTGGCGGATCAGGCGGGCACGGTGATCCTGCAGATTCGCGACGGTGCCAGCGAGG
CGGTAGAGGCCGTGAGCATGTTCGCCAACGAACGCGGCGCCCCCTAACATTGGAAGTGGA
TAACGGATCCGAATTC

Protein sequence predicted based on nucleotide sequence

MLFNRHNIITDSLQQTIAEQSRLLEAIDRSMAVIEFDLQGNVLRANANFLQTLGYREDQV
TGQPHRLFCTPEYVRSSDYQQLWNRLRSGQFESGTFERVASNGQSLWLEASYNPIRDASG
AVYKVVKYALDVTARVQEEVEARNKLDAIDRAMAVIEFNLDGSIIGANSNFLSRMGYSLA
EIKGKHHRMFCKPELINSSAYQDFWNRLNRGEFFNGQFERIDRHGRTLWLEANYNPVYDA
SGRLCKVVKYAADVTQQVEKHEQDAQSASQAYHISVQTRKVAEQGTGVIQQAASEMRQIA
ENIEGSSSLIARLGERSEQITAIVNTIRAIADQTNLLALNAAIEAARAGDQGRGFAVVAD
EVRQLAARTSGSTAEISSMIDMIQNETQQAIKSMDGTRERAAKGVQLADQAGTVILQIRD
GASEAVEAVSMFANERGAP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a23t,g24t,K8I;c26t,T9I
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC67807.0
Recommended expression in:
Not Applicable