BT_0428 (B. thetaiotaomicron VPI-5482 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_0428
Gene Name:  phenylacetate-coenzyme A ligase
Original Clone ID:
48575
Keyword:
full-length cds
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Identical to BtCD00531927
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
BtCD00452673
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UNAVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1308nts
Open reading frame: 51 to 1358

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAGTACAC
AATACTGGGAAGAAGAAATTGAAATCATGAGTCGCGAGAAGTTGCAGGAACTGCAACTTC
AGCGACTCAAAAAAACGATTAACATAGCCGCAAACTCACCTTATTACAAAGAGGTTTTCA
GCAAAAACGGCATCACCGGAGATAGTATCCAGTCACTGGACGACATCCGCAAAATTCCTT
TCACCACTAAATCGGACATGCGTGCCAACTATCCCTTCGGGCTAGTGGCCGGCGACATGA
AAAGAGACGGTGTACGTATCCACTCTTCCAGTGGAACTACAGGAAACCCTACAGTCATCG
TACATTCACAACATGACCTCGACTCATGGGCAAATCTGGTAGCCCGTTGCCTTTACATGG
TGGGGATCCGCAAGACAGATGTCTTCCAGAACAGCTCCGGATATGGAATGTTTACAGGTG
GATTAGGTTTTCAATATGGTGCAGAACGCCTTGGCTGCCTTACCGTACCTGCCGCAGCCG
GAAACAGTAAACGGCAAATTAAATTCATCAGTGACTTTAAGACTACCGCCTTACACGCCA
TCCCAAGCTACGCTATCCGTCTTGCCGAAGTATTTCAGGAAGAAGGTATCGACCCGAGAG
AAACAACCCTGAAAACCTTGGTGATCGGCGCCGAACCGCATACTGACGAACAGCGCCGTA
AAATAGAACGGATGCTCAATGTGAAAGCATATAATAGTTTCGGTATGACGGAAATGAACG
GCCCCGGTGTTGCCTTCGAATGCCAGGAGCAGAACGGAATGCACTTCTGGGAAGATTGCT
ATCTGGTAGAAATCATCGATCCTGAAACAGGAGAACCCGTTCCCGAAGGAGAAATCGGTG
AATTGGTACTCACTACACTTGATCGTGAAATGATGCCGCTTATCCGCTACCGCACCCGCG
ACCTTACCCGTATACTACCCGGAAAATGTCCGTGCGGCCGCACCCATCTCCGCATCGACC
GGATCAAGGGACGCAGCGACGATATGTTTATCATCAAGGGAGTCAACATCTTCCCCATGC
AAGTCGAAAAGATTCTGGTTCAGTTCCCCGAATTAGGCAGCAATTATCTGATTACGCTGG
AGACAGTCAACAATCAGGATGAGATGATCGTCGAAGTGGAATTAAGCGATCTTTCTACAG
ACAACTATATCGAACTGGAAAAGATCCGCAGAGATATTATCCGTCAATTAAAAGATGAAA
TACTGGTTACCCCGAAAGTAAAACTAGTAAAGAAAGGGTCTCTCCCGCAGAGCGAGGGAA
AAGCCGTCAGAGTGAAGGACTTAAGAGACAACAAATAACGCGACTTAATTAATTTGGCGG
ATGAGAGAAGATTTTCAGCCTGATACAGA

Protein sequence predicted based on nucleotide sequence

MSTQYWEEEIEIMSREKLQELQLQRLKKTINIAANSPYYKEVFSKNGITGDSIQSLDDIR
KIPFTTKSDMRANYPFGLVAGDMKRDGVRIHSSSGTTGNPTVIVHSQHDLDSWANLVARC
LYMVGIRKTDVFQNSSGYGMFTGGLGFQYGAERLGCLTVPAAAGNSKRQIKFISDFKTTA
LHAIPSYAIRLAEVFQEEGIDPRETTLKTLVIGAEPHTDEQRRKIERMLNVKAYNSFGMT
EMNGPGVAFECQEQNGMHFWEDCYLVEIIDPETGEPVPEGEIGELVLTTLDREMMPLIRY
RTRDLTRILPGKCPCGRTHLRIDRIKGRSDDMFIIKGVNIFPMQVEKILVQFPELGSNYL
ITLETVNNQDEMIVEVELSDLSTDNYIELEKIRRDIIRQLKDEILVTPKVKLVKKGSLPQ
SEGKAVRVKDLRDNK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC
3' Linker Sequence:
TAACGCGACTTAATTAA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 396691
Recommended expression in:
Not Applicable