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CAPN2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN2
Gene Name:  calpain 2, (m/II) large subunit
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH196034.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00045329

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2103nts         Open reading frame : 1 to 2103
Reference Sequence Alignment

ATGGCGGGCATCGCGGCCAAGCTGGCGAAGGACCGGGAGGCGGCCGAGGGGCTGGGCTCC
CACGAGAGGGCCATCAAGTACCTCAACCAGGACTACGAGGCGCTGCGGAACGAGTGCCTG
GAGGCCGGGACGCTCTTCCAGGACCCGTCCTTCCCGGCCATCCCCTCGGCCCTGGGCTTC
AAGGAGTTGGGGCCCTACTCCAGCAAAACCCGGGGCATCGAGTGGAAGCGCCCCACGGAG
ATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGCCAAGGA
GCCCTAGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAAGAAATC
CTGGCTCGAGTCGTCCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATCTTTCAC
TTCCAGTTCTGGCAATACGGCGAGTGGGTGGAGGTGGTGGTGGATGACAGGCTGCCCACC
AAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCGAAGGGAGCGAGTTCTGGAGCGCCCTG
CTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCACTATCAGGGGGTGCCACC
ACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAGAAGCCC
CCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGC
TCCATCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTGGTGAAG
GGGCACGCGTACTCGGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTACAGAAA
CTGATCCGCATCCGAAATCCCTGGGGAGAAGTGGAGTGGACAGGGCGGTGGAATGACAAC
TGCCCAAGCTGGAACACTATAGACCCAGAGGAGAGGGAAAGGCTGACCAGACGGCATGAA
GATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTATTCCCGCCTGGAGATC
TGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTCACCAAA
ATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCGAACACA
TTCTGGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAGGAGGAT
GGGGAGAGCGGCTGCACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGGCAGAGG
AAGATGGGCGAGGACATGCACACCATCGGCTTTGGCATCTATGAGGTTCCAGAGGAGTTA
AGTGGGCAGACCAACATCCACCTCAGCAAAAACTTCTTCCTGACGAATCGCGCCAGGGAG
CGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTTCAAGCTGCCGCCAGGA
GAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGCATCCGG
GTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCCAATCTT
GAAGAGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTTGCCCAG
TTGGCAGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGAAGGGTT
CTAGCAAAGCGCCAAGATATCAAGTCAGATGGCTTCAGCATCGAGACATGCAAAATTATG
GTTGACATGCTAGATTCGGACGGGAGTGGCAAGCTGGGGCTGAAGGAGTTCTACATTCTC
TGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGACGTTGACAGGTCTGGT
ACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATGCCCTGT
CAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGATTTTGAT
AATTTTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAGCTGGAT
CCCGAGAATACTGGAACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCAGTACTT
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2230
Start on reference sequence 128
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN2
Symbol Nomenclature : CAPN2
SYNONYM : CANP2; CANPL2; CANPml; mCANP
Designation : CANP 2|M-calpain|calcium-activated neutral proteinase 2|calpain 2, large [catalytic] subunit|calpain 2, large subunit|calpain M-type|calpain large polypeptide L2|calpain, large polypeptide L2|calpain-2 catalytic subunit|calpain-2 large subunit|millimolar-calpain
Full Nomenclature : calpain 2, (m/II) large subunit
GENEID : 824
HGNC : 1479
MIM : 114230
Vega : OTTHUMG00000037376
Target GenBank: BC021303

 

Reference Sequence Alignment

  

HsCD00045329      ATGGCGGGCATCGCGGCCAAGCTGGCGAAGGACCGGGAGGCGGCCGAGGGGCTGGGCTCC
NM_001748.4       ATGGCGGGCATCGCGGCCAAGCTGGCGAAGGACCGGGAGGCGGCCGAGGGGCTGGGCTCC
                  ************************************************************

HsCD00045329      CACGAGAGGGCCATCAAGTACCTCAACCAGGACTACGAGGCGCTGCGGAACGAGTGCCTG
NM_001748.4       CACGAGAGGGCCATCAAGTACCTCAACCAGGACTACGAGGCGCTGCGGAACGAGTGCCTG
                  ************************************************************

HsCD00045329      GAGGCCGGGACGCTCTTCCAGGACCCGTCCTTCCCGGCCATCCCCTCGGCCCTGGGCTTC
NM_001748.4       GAGGCCGGGACGCTCTTCCAGGACCCGTCCTTCCCGGCCATCCCCTCGGCCCTGGGCTTC
                  ************************************************************

HsCD00045329      AAGGAGTTGGGGCCCTACTCCAGCAAAACCCGGGGCATCGAGTGGAAGCGCCCCACGGAG
NM_001748.4       AAGGAGTTGGGGCCCTACTCCAGCAAAACCCGGGGCATCGAGTGGAAGCGCCCCACGGAG
                  ************************************************************

HsCD00045329      ATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGCCAAGGA
NM_001748.4       ATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGCCAAGGA
                  ************************************************************

HsCD00045329      GCCCTAGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAAGAAATC
NM_001748.4       GCCCTGGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAAGAAATC
                  *****.******************************************************

HsCD00045329      CTGGCTCGAGTCGTCCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATCTTTCAC
NM_001748.4       CTGGCTCGAGTCGTCCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATCTTTCAC
                  ************************************************************

HsCD00045329      TTCCAGTTCTGGCAATACGGCGAGTGGGTGGAGGTGGTGGTGGATGACAGGCTGCCCACC
NM_001748.4       TTCCAGTTCTGGCAATACGGCGAGTGGGTGGAGGTGGTGGTGGATGACAGGCTGCCCACC
                  ************************************************************

HsCD00045329      AAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCGAAGGGAGCGAGTTCTGGAGCGCCCTG
NM_001748.4       AAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCGAAGGGAGCGAGTTCTGGAGCGCCCTG
                  ************************************************************

HsCD00045329      CTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCACTATCAGGGGGTGCCACC
NM_001748.4       CTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCGCTATCAGGGGGTGCCACC
                  *****************************************.******************

HsCD00045329      ACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAGAAGCCC
NM_001748.4       ACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAGAAGCCC
                  ************************************************************

HsCD00045329      CCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGC
NM_001748.4       CCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGC
                  ************************************************************

HsCD00045329      TCCATCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTGGTGAAG
NM_001748.4       TCCATCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTGGTGAAG
                  ************************************************************

HsCD00045329      GGGCACGCGTACTCGGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTACAGAAA
NM_001748.4       GGGCACGCGTACTCGGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTACAGAAA
                  ************************************************************

HsCD00045329      CTGATCCGCATCCGAAATCCCTGGGGAGAAGTGGAGTGGACAGGGCGGTGGAATGACAAC
NM_001748.4       CTGATCCGCATCCGAAATCCCTGGGGAGAAGTGGAGTGGACAGGGCGGTGGAATGACAAC
                  ************************************************************

HsCD00045329      TGCCCAAGCTGGAACACTATAGACCCAGAGGAGAGGGAAAGGCTGACCAGACGGCATGAA
NM_001748.4       TGCCCAAGCTGGAACACTATAGACCCAGAGGAGAGGGAAAGGCTGACCAGACGGCATGAA
                  ************************************************************

HsCD00045329      GATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTATTCCCGCCTGGAGATC
NM_001748.4       GATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTATTCCCGCCTGGAGATC
                  ************************************************************

HsCD00045329      TGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTCACCAAA
NM_001748.4       TGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTCACCAAA
                  ************************************************************

HsCD00045329      ATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCGAACACA
NM_001748.4       ATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCGAACACA
                  ************************************************************

HsCD00045329      TTCTGGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAGGAGGAT
NM_001748.4       TTCTGGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAGGAGGAT
                  ************************************************************

HsCD00045329      GGGGAGAGCGGCTGCACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGGCAGAGG
NM_001748.4       GGGGAGAGCGGCTGCACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGGCAGAGG
                  ************************************************************

HsCD00045329      AAGATGGGCGAGGACATGCACACCATCGGCTTTGGCATCTATGAGGTTCCAGAGGAGTTA
NM_001748.4       AAGATGGGCGAGGACATGCACACCATCGGCTTTGGCATCTATGAGGTTCCAGAGGAGTTA
                  ************************************************************

HsCD00045329      AGTGGGCAGACCAACATCCACCTCAGCAAAAACTTCTTCCTGACGAATCGCGCCAGGGAG
NM_001748.4       AGTGGGCAGACCAACATCCACCTCAGCAAAAACTTCTTCCTGACGAATCGCGCCAGGGAG
                  ************************************************************

HsCD00045329      CGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTTCAAGCTGCCGCCAGGA
NM_001748.4       CGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTTCAAGCTGCCGCCAGGA
                  ************************************************************

HsCD00045329      GAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGCATCCGG
NM_001748.4       GAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGCATCCGG
                  ************************************************************

HsCD00045329      GTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCCAATCTT
NM_001748.4       GTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCCAATCTT
                  ************************************************************

HsCD00045329      GAAGAGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTTGCCCAG
NM_001748.4       GAAGAGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTTGCCCAG
                  ************************************************************

HsCD00045329      TTGGCAGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGAAGGGTT
NM_001748.4       TTGGCAGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGAAGGGTT
                  ************************************************************

HsCD00045329      CTAGCAAAGCGCCAAGATATCAAGTCAGATGGCTTCAGCATCGAGACATGCAAAATTATG
NM_001748.4       CTAGCAAAGCGCCAAGATATCAAGTCAGATGGCTTCAGCATCGAGACATGCAAAATTATG
                  ************************************************************

HsCD00045329      GTTGACATGCTAGATTCGGACGGGAGTGGCAAGCTGGGGCTGAAGGAGTTCTACATTCTC
NM_001748.4       GTTGACATGCTAGATTCGGACGGGAGTGGCAAGCTGGGGCTGAAGGAGTTCTACATTCTC
                  ************************************************************

HsCD00045329      TGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGACGTTGACAGGTCTGGT
NM_001748.4       TGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGACGTTGACAGGTCTGGT
                  ************************************************************

HsCD00045329      ACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATGCCCTGT
NM_001748.4       ACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATGCCCTGT
                  ************************************************************

HsCD00045329      CAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGATTTTGAT
NM_001748.4       CAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGATTTTGAT
                  ************************************************************

HsCD00045329      AATTTTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAGCTGGAT
NM_001748.4       AATTTTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAGCTGGAT
                  ************************************************************

HsCD00045329      CCCGAGAATACTGGAACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCAGTACTT
NM_001748.4       CCCGAGAATACTGGAACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCAGTACTT
                  ************************************************************

HsCD00045329      TAG
NM_001748.4       TGA
                  *..

Pathways

NCI : ErbB1 downstream signaling
NCI : Signaling events mediated by focal adhesion kinase
Panther : Huntington disease
WikiPathway : Alzheimers Disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
UniProt : Q59EF6
UniProt : P17655
UniProt : B4DN77
UniProt : B7ZA96
HPRD : 00254

Clone

Cloning Information : 196034
HIP Master Clone ID : 170745
Original Clone ID : FLH196034.01X

Organism


TAX_ID : 9606
Species Specific ID: 824

Genome

Chromosome : 1
Map Location : 1q41-q42
Ensembl : ENSG00000162909

Reagents

Labome : CAPN2-antibody