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KRT10 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KRT10
Gene Name:  keratin 10
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH196549.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 24430336
Title: The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold
Authors: HIP
HsCD00045373

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1755nts         Open reading frame : 1 to 1755

ATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGGAGGAGGAGGA
GGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTCTAGCAGCAAA
GGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTTTAGCCGTGGG
AGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATTAGGAGGTTTT
GGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAGTTATGGAGGC
AGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTTTGGTGGAGGC
GGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGGAGATGGTGGC
CTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCTGGCTTCCTAC
TTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAAAATCAAGGAG
TGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAGCAAATACTAC
AAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAATGCCAACATC
CTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAAGTATGAGAAT
GAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTAGGGTGCTGGAT
GAGCTGACCCTGACCAAGGCTGACCTGGAGATGCAAATTGAGAGCCTGACTGAAGAGCTG
GCCTATCTGAAGAAGAACCACGAGGAGGAAATGAAAGACCTTCGAAATGTGTCCACTGGT
GATGTGAATGTGGAAATGAATGCTGCCCCGGGTGTTGATCTGACTCAACTTCTGAATAAC
ATGAGAAGCCAATATGAACAACTTGCTGAACAAAACCGCAAAGATGCTGAAGCCTGGTTC
AATGAAAAGAGCAAGGAACTGACTACAGAAATTGATAATAACATTGAACAGATATCCAGC
TATAAATCTGAGATTACTGAATTGAGACGTAATGTACAAGCTCTGGAGATAGAACTACAG
TCCCAACTGGCCTTGAAACAATCCCTGGAAGCCTCCTTGGCAGAAACAGAAGGTCGCTAC
TGTGTGCAGCTCTCACAGATTCAGGCCCAGATATCCGCTCTGGAAGAACAGTTGCAACAG
ATTCGAGCTGAAACCGAGTGCCAGAATACTGAATACCAACAACTCCTGGATATTAAGATC
CGACTGGAGAATGAAATTCAAACCTACCGCAGCCTGCTAGAAGGAGAGGGAAGTTCCGGA
GGCGGCGGACGCGGCGGCGGAAGTTTCGGCGGCGGCTACGGCGGCGGAAGCTCCGGCGGC
GGAAGCTCCGGCGGCGGCTACGGCGGCGGCCACGGCGGCAGTTCCGGCGGCGGCTACGGA
GGCGGAAGCTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGGGGCGGAAGCTCCAGCGGC
GGCCACGGCGGCAGTTCCAGCGGCGGCTACGGTGGTGGCAGTTCCGGCGGCGGCGGCGGC
GGCTACGGGGGCGGCAGCTCCGGCGGCGGCAGCAGCTCCGGCGGCGGATACGGCGGCGGC
AGCTCCAGCGGAGGCCACAAGTCCTCCTCTTCCGGGTCCGTGGGCGAGTCTTCATCTAAG
GGACCAAGATACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1765
Start on reference sequence 11
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KRT10
Symbol Nomenclature : KRT10
SYNONYM : BCIE; BIE; CK10; EHK; K10; KPP
Designation : CK-10|cytokeratin 10|keratin, type I cytoskeletal 10|keratin-10
Full Nomenclature : keratin 10
GENEID : 3858
HGNC : 6413
MIM : 148080
Vega : OTTHUMG00000133368
Target GenBank: BC034697

 

Protein

UniProt : P13645
HPRD : 01021

Clone

Cloning Information : 196549
HIP Master Clone ID : 171260
Original Clone ID : FLH196549.01X

Organism


TAX_ID : 9606
Species Specific ID: 3858

Genome

Chromosome : 17
Map Location : 17q21
Ensembl : ENSG00000186395

Reagents

Labome : KRT10-antibody