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ydhD (B. licheniformis DSM 13 = ATCC 14580 ) in pMCSG7

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Explanation of Terms


Gene: Gene Symbol:  yaaH
Gene Name:  glycoside hydrolase family protein YaaH
Sequence             
Original Clone ID: APC100022.102
Keyword: None
Species: Bacillus licheniformis DSM 13 = ATCC 14580
Type: cDNA
Vector Name: pMCSG7               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Shonda Clancy
PSI:Biology
Midwest Center for Structural Genomics
BlCD00454158

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1320nts        
Open reading frame : 43 to 1362

GTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGAAAGGAGGCGTTT
CCCATTCAGATTTATGTCGTGAAAAGAGGCGATACCCTTTACCAAATCGCGAATCGTTAC
CGAACAACAGTTAATGAAATTGTCGCGACGAATGAAATTCCGAACCCGAATCGGCTTGTT
GTCGGACAAACCATCGTGATCCCGATCGCCGGCGAGTTTTATGAGGTCAGACAGGGAGAT
ACATTAGCATCAATCGGAGCACGCTTTAATATTTCTCCGGCTGAACTGGCGAGGATCAAC
CGCATTCAGGTTAGCGCCGTTTTACCGGTCGGACTGCTGCTTTACATCCCCCCTCGGCCA
AGACGGAACATTGAAACAAACGCCTATATCGAACCTCGGGGAGAAAGCGTAAGCCCCGCT
TTGCAGCAGGCGGCAAGAGAGGCTTCGCCATACTTGACCTATCTGGGCGCTTTCAGCTTC
CAGGCGAAGCGGGACGGCACACTCGAAGAACCGCCGCTGAACAACTTAAAAGAAATTGCC
GACAGACATCGGACTACGATGATGATGATTGTCACCAATCTCGAAAATGAAGCTTTCAGC
GACGAACTCGGCAGGATCATTCTGACGGACCAGAATGTAAAAAACAGATTGCTCGATAAC
ATCGTTGCAGCGGCCAGAAGATACGGTTTTAAAGACATCCATTTTGACTTCGAATACTTA
AGGCCCGAAGATAGAGAAGCCTATAATCAGTTTTTACGGGATGCCCGGGCCCGCTTCAGG
CAGGAAGGCTGGCTCATCTCAACCGCGCTTGCTCCGAAAACGAGAGCGGATCAGCCCGGA
CAATGGTATGAAGCCCATGATTACCGGGCCCACGGCGAAATTGTCGATTTCGTCGTGCTG
ATGACATATGAGTGGGGCTACAGCGGAGGGCCCCCGATGGCGGTATCGCCGATCGGGCCC
GTCCGGGACGTGATCGAATACGCACTCACGGAAATGCCCGCCAGCAAAATCGTCATGGGA
CAGAACTTGTACGGCTATGACTGGACGCTCCCCTATACGCCAGGCGGACCGCTGGCCAGA
GCAATCAGCCCCCAGCGGGCGATTGAGATCGCGAGCGAGAACAATGCGGCGATACAGTAC
GATGAAACAGCTCAGGCTCCAAACTTCCGCTATACGGACAATGCCGGCAAACAGCATGAA
GTATGGTTTGAAGACGCCCGCTCGATTCAGGCAAAGTTTGATCTGATTAGGGAATTGAAT
TTAAGGGGAATCAGCTATTGGAAGCTCGGCCTGTCCTTCCCGCAAAACTGGCTCTTGATA
GCCGATCAATTTAATGTTGTTAAAGAGACGTTCCCAAGTTAATAACATTGGAAGTGGATA
A


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100022
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pMCSG7

pMCSG7 in advanced viewed

Synonyms: pMCSG-7
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Comments: pET21 derivative; for routine HTP purification
Size (bp): 5286
Parent Vector: None
Empty Vector: EvNO00084271
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 204 235
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 3280 3280
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 335 357
repressor protein gene LacI LacI coding region 890 1849
selectable marker AmpR ampicillin resistance gene 4038 4898
tag His 2 N-terminal 6xHis tag 268 285
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : yaaH
GENEID : 3097556
Locus Tag : BLi00025
GI : 52783874
Target GenBank: None

 

Protein

SMART domain : Glyco_18 :
SMART domain : LysM : Lysin motif
Structural Annotation Wiki : TOPSAN
Structural Annotation Wiki : MCSG-APC100022

Clone

Cloning Information : 583304
HIP Master Clone ID : 486233
Original Clone ID : APC100022.102

Experimental

TargetTrack - Experimental data : MCSG-APC100022

Organism


TAX_ID : 279010
Species Specific ID: 3097556

Reagents

Labome : yaaH-cDNA