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pbg1 (Lactobacillus plantarum WCFS1) in pMCSG7 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  pbg1
Gene Name:  6-phospho-beta-glucosidase
Sequence             
Original Clone ID: APC100114.102
Keyword: None
Species: Lactobacillus plantarum WCFS1
Type: cDNA
Vector Name: pMCSG7               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23519420
Title: GH1-family 6-P-Beta-glucosidases from human microbiome lactic acid bacteria.
Authors: Midwest Center for Structural Genomics
Jessica Bearden
PSI:Biology
LpCD00454237

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1437nts         Open reading frame : 44 to 1480

TGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACGATTAAAGGACG
AGCGTTTCCAGAAGGGTTCTTATGGGGTGGCGCAGTGGCTGCACATCAACTCGAAGGTGG
TTATAAAGAGGGCGGCAAGGGTCTCAGTACGGCAGATATTATGACATTAGGGACGAATGA
GCGCCCTCGTGAAATTACGGACGGAGTAGTGGCTGGCAAGTATTATCCTAATCATCAGGC
AATTGATTTTTATCATCGTTATCCCGAAGATATTGAATTATTTGCTGAAATGGGGTTTAA
GTGTTTTCGAACATCGATTGCCTGGACGCGGATCTTCCCCAATGGTGATGAAAGCGAACC
GAATGAAGCTGGATTACAATTTTATGATGATTTATTTGATGAATGCCTTAAAAATGGGAT
TCAACCAGTAGTGACACTAGCACACTTTGAAATGCCATATCACTTAGTTAAGCAATACGG
TGGGTGGCGTAATCGTAAGCTTATTCAATTCTATTTAAACTTTGCAAAAGTATGTTTTGA
ACGTTATCGAGATAAAGTAACGTACTGGATGACTTTTAATGAAATCAACAATCAAACTAA
TTTTGAATCAGACGGTGCCATGTTAACAGATTCTGGAATCATTCATCAACCAGGAGAAAA
CCGTGAACGCTGGATGTATCAGGCGGCTCACTATGAACTGGTGGCGAGTGCCGCGGCGGT
ACAGTTAGGCCATCAGATTAATCCAGATTTTCAAATCGGTTGTATGATTGCGATGTGTCC
GATCTATCCGCTTACAGCAGCACCAGCAGACGTCTTATTTGCGCAACGAGCGATGCAAAC
CCGATTTTATTTTGCCGATGTTCATTGCAATGGCACTTACCCACAATGGTTGCGTAATCG
TTTTGAGTCGGAGCATTTTAACTTGGACATTACGGCAGAAGATTTGAAAATCTTGCAAGC
TGGAACGGTAGATTATATTGGATTTAGCTATTACATGTCGTTTACTGTGAAGGACACAGG
AAAGCTAGCTTATAATGAAGAACATGATTTAGTTAAAAATCCATATGTTAAGGCTAGTGA
TTGGGGCTGGCAAGTTGATCCCGTTGGGTTACGCTATGCTATGAATTGGTTCACGGATCG
TTACCATTTGCCACTGTTTATCGTGGAAAACGGTTTAGGCGCAATTGATAAGAAGACGGC
AGATAACCAGATACATGACGATTATCGGATTGACTATTTAACGGATCATTTACGACAAAT
TAAGCTGGCAGTTCTGGAAGATGGGGTTGATTTAATTGGGTATACGCCATGGGGGTGCAT
TGATTTAGTTGCGGCTAGTACTGGTCAAATGTCCAAACGCTATGGGTTTATCTATGTTGA
TGAGAACGATGATGGTTCAGGTAGTCTAAAACGTTATAAAAAGGATTCATTTACTTGGTT
TCAACATGTGATTGCGACAAATGGTGCCGAAATTGAGTAGTAACATTGGAAGTGGATAAC
GGATCCGAATTCGAGCTCCG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100114
Recommended expression in: E.coli BL21 (DE3)
       DyNA Vector Map

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Vector Name: pMCSG7

pMCSG7 in advanced viewed

Synonyms: pMCSG-7
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Comments: pET21 derivative; for routine HTP purification
Size (bp): 5286
Parent Vector: None
Empty Vector: EvNO00084271
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 204 235
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 3280 3280
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 335 357
repressor protein gene LacI LacI coding region 890 1849
selectable marker AmpR ampicillin resistance gene 4038 4898
tag His 2 N-terminal 6xHis tag 268 285
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : pbg1
GENEID : 1064159
Locus Tag : lp_0440
GI : 28377342
Target GenBank: None

 

Protein

PDB - Structural Annotation : 4GZE
PDB - Structural Annotation : 3QOM
Structural Annotation Wiki : MCSG-APC100114
Structural Annotation Wiki : TOPSAN
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 583467
HIP Master Clone ID : 486396
Original Clone ID : APC100114.102

Experimental

Structural Biology Knowledgebase : SBKB (4GZE)
Structural Biology Knowledgebase : SBKB (3QOM)
TargetTrack - Experimental data : MCSG-APC100114

Organism


TAX_ID : 220668
Species Specific ID: 1064159

Reagents

Labome : pbg1-cDNA