DNASU Plasmid Repository • 480.965.5697 | Email

Aaci_1878 (A. acidocaldarius subsp. acidocaldarius DSM 446 ) in pMCSG19

TOP

Explanation of Terms


Gene: Gene Symbol:  Aaci_1878
Gene Name:  MarR family transcriptional regulator
Sequence             
Original Clone ID: APC101012.103
Keyword: None
Species: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Type: cDNA
Vector Name: pMCSG19               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Midwest Center for Structural Genomics
Shonda Clancy
PSI:Biology
AaCD00454682

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 477nts         Open reading frame : 51 to 527

CTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGAACGGGG
ATCGCCTGGATATCGCACAGCGGCTCGACACGGTCATGGAGCGGTTCTTTCGCGGGATGC
GCTCGCACTTCACCGAGGACAAATTCGGTTTGACACACACGCAGGCGTTTGTGCTCTGGC
ACCTGAGCCTCGTGCCGCAGGCCAAATCGTCTGACCTGGCCCGCATCGCAGGTCTGAGCC
CCGGCGCCATCACCCAGGTGTGTGACGAACTGGTCCGATTGGGTTACGTCGAGCGCGTGC
GTTCGAGCGAGGATCGGCGCGTCGTATACGTGATTCTGACGGATCGCGGCCGTTCCCAAC
TCCGGGAGCTGACGAGAAATCGCGCCGTCAAGATTGCGCAACTCCTGGACAAGCTGGGGG
AACACGACGCGAGGAAGTTCCTCGAGCTTCTCGAGCGATTGGTCGCCATCATGGATGAAC
ACCCATGTGGAGCGAGTCCGCGTGCCAGACAGGAGGGTGAACCATAACATTGGAAGTGGA
TAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC101012
Recommended expression in: E.coli BL21 (DE3)
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pMCSG19

pMCSG19 in advanced viewed

Synonyms: pMCSG-19
Sequencing Primer: Forward:  ANForward
Reverse:  T7 terminator
Description: Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative: in vivo TVMV cleavage of MBP
Size (bp): 6441
Parent Vector: None
Empty Vector: EvNO00084265
Properties: bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 204 235
bacterial origin ori ColE1-type bacterial origin of replication 4416 5098
promoter T7 T7 promoter 1515 1531
protease cleavage site TVMV TVMV protease cleavage site 286 306
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 1490 1512
repressor protein gene LacI LacI coding sequence 2045 3004
selectable marker AmpR ampicillin resistance gene 5193 6053
tag His 2 C-terminal 6xHis tag 140 157
tag His 6xHis tag 268 285
tag MBP N-terminal MBP 337 1443
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : Aaci_1878
GENEID : 8425398
Locus Tag : Aaci_1878
GI : 257478571
Target GenBank: None

 

Clone

Cloning Information : 584261
HIP Master Clone ID : 487190
Original Clone ID : APC101012.103

Experimental

TargetTrack - Experimental data : MCSG-APC101012

Organism


TAX_ID : 521098
Species Specific ID: 8425398

Reagents

Labome : Aaci_1878-cDNA