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Detailed Vector Information: pMCSG51

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG51              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter, N-terminal 6xHis tag adjacent to biotinylation site (AviTag) followed by TEV protease cleavage site; expresses biotin ligase (BirA); amipicillin resistance in bacteria; ligation independent cloning.
Comments: None
Publications: None
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00454844

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG51

pMCSG51 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter, N-terminal 6xHis tag adjacent to biotinylation site (AviTag) followed by TEV protease cleavage site; expresses biotin ligase (BirA); amipicillin resistance in bacteria; ligation independent cloning.
Comments: Origin of replication similar to pUC vectors
Size (bp): 5346
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 3339 3927
binding site biotinylation Biotinylation signal 1278 1322
gene fragment BirA biotin ligase (BirA) gene 244 1206
ligation independent cloning LIC MCSG7 LIC site 1232 1263
promoter T7 T7 promoter 1421 1437
protease cleavage site TEV TEV protease cleavage site 1251 1271
repressor protein gene LacI Lac I 1951 2910
ribosome binding site RBS2 ribosome binding site 1358 1363
ribosome binding site RBS ribosome binding site 1215 1220
selectable marker AmpR ampicillin resistance gene 4098 4958
tag His N-terminal 6xHis tag 1329 1346
trxn termination sequence T7 term T7 terminator 26 72

 

Species Specific ID: None
Target GenBank: None