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Detailed Vector Information: pMCSG56

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG56              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with T7 promoter and N-terminal 6xHis and double HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: None
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00454847

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pMCSG56

pMCSG56 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and N-terminal 6xHis and double HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
Comments: origin of replication similar to pUC vectors
Size (bp): 4332
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR bacterial origin of replication 2325 2913
ligation independent cloning LIC ligation independent cloning site 204 235
promoter T7 T7 promoter 414 430
protease cleavage site TEV TEV protease cleavage site 223 243
repressor protein gene LacI Lac I 944 1903
selectable marker AmpR ampicillin resistance gene 3084 3944
tag HA N-terminal double HA tag 250 303
tag His N-terminal 6xHis tag 322 339
trxn regulatory element lac operator lac operator sequence 389 411
trxn termination sequence T7 term T7 terminator 26 72

 

Species Specific ID: None
Target GenBank: None