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Detailed Vector Information: pMCSG60

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG60              
Source: Midwest Center for Structural Genomics
Description: Bacterial co-expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag (before first LIC site); ampicillin resistance in bacteria; ligation independent cloning
Comments: None
Publications: None
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00454849

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG60

pMCSG60 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial co-expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag (before first LIC site); ampicillin resistance in bacteria; ligation independent cloning
Comments: Dual LIC expression vector based on pMCSG7. No His tag on second LIC site
Size (bp): 5342
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR332 origin of replication 3336 3336
ligation independent cloning LIC pMCSG7 ligation independent cloning site 260 291
ligation independent cloning LIC2 pMCSG26 ligation independent cloning site 223 244
promoter T7 T7 promoter 416 432
protease cleavage site TEV TEV protease cleavage site 279 299
repressor protein gene LacI Lac I 946 1905
ribosome binding site RBS ribosomal binding site 243 248
ribosome binding site RBS2 ribosomal binding site 353 358
selectable marker AmpR ampicillin resistance gene 4094 4954
tag His N-terminal 6xHis tag (for first LIC site) 324 341
trxn termination sequence T7 term T7 terminator 26 72

 

Species Specific ID: None
Target GenBank: None