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CAPN10 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN10
Gene Name:  calpain 10
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH194931.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00045536

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2019nts         Open reading frame : 1 to 2019
Reference Sequence Alignment

ATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGAGCTGTTCCGGGACGCCGCCTTCCCC
GCCGCGGACTCCTCGCTCTTCTGCGACTTGTCTACGCCGCTGGCCCAGTTCCGCGAGGAC
ATCACGTGGAGGCGGCCCCAGGAGATTTGTGCCACACCCCGGCTGTTTCCAGATGACCCA
CGGGAAGGGCAGGTGAAGCAGGGGCTGCTGGGGGATTGCTGGTTCCTGTGTGCCTGCGCC
GCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGTCATTCCTCCGGGACAGCCGAGCTGG
GCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCGCATTTGGCAGTTTGGACGCTGGGTG
GAGGTGACCACAGATGACCGCCTGCCGTGCCTTGCAGGGAGACTCTGTTTCTCCCGCTGC
CAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGAAAAGGTCTACGCCAAGGTCCATGGG
TCCTACGAGCACCTGTGGGCCGGGCAGGTGGCGGATGCCCTGGTGGACCTGACCGGCGGC
CTGGCAGAAAGATGGAACCTGAAGGGCGTAGCAGGAAGCGGAGGCCAGCAGGACAGGCCA
GGCCGCTGGGAGCACAGGACTTGTCGGCAGCTGCTCCACCTGAAGGACCAGTGTCTGATC
AGCTGCTGCGTGCTCAGCCCCAGAGCAGGTGCCCGGGAGCTGGGGGAGTTCCATGCCTTC
ATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGCGGGCCAGTGCATCCTGCTGCTGCGG
ATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGGGCTCTGGAGAGAGGGGGGTGAAGGG
TGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCTCCTGTCCCAGCTCCAGGAAGGGGAG
TTCTGGGTGGAGGAGGAGGAGTTCCTCAGGGAGTTTGACGAGCTCACCGTTGGCTACCCG
GTCACGGAGGCCGGCCACCTGCAGAGCCTCTACACAGAGAGGCTGCTCTGCCATACGCGG
GCGCTGCCTGGGGCCTGGGTCAAGGGCCAGTCAGCAGGAGGCTGCCGGAACAACAGCGGC
TTTCCCAGCAACCCCAAATTCTGGCTGCGGGTCTCAGAACCGAGTGAGGTGTACATTGCC
GTCCTGCAGAGATCCAGGCTGCACGCGGCGGACTGGGCAGGCCGGGCCCGGGCACTGGTG
GGTGACAGTCATACTTCGTGGAGCCCAGCGAGCATCCCGGGCAAGCACTACCAGGCTGTG
GGTCTGCACCTCTGGAAGGTAGAGAAGCGGCGGGTCAATCTGCCTAGGGTCCTGTCCATG
CCCCCCGTGGCTGGCACCGCGTGCCATGCATACGACCGGGAGGTCCACCTGCGTTGTGAG
CTCTCACCGGGCTACTACCTGGCTGTCCCCAGCACCTTCCTGAAGGACGCGCCAGGGGAG
TTCCTGCTCCGAGTCTTCTCTACCGGGCGAGTCTCCCTTAGCGCCATCAGGGCAGTGGCC
AAGAACACCACCCCCGGGGCAGCCCTGCCTGCGGGGGAGTGGGGGACCGTGCAGCTACGG
GGTTCTTGGAGAGTCGGCCAGACGGCGGGGGGCAGCAGGAACTTTGCCTCATACCCCACC
AACCCCTGCTTCCCCTTCTCGGTCCCCGAGGGCCCTGGCCCCCGCTGCGTCCGCATCACT
CTGCATCAGCACTGCCGGCCCAGTGACACCGAGTTCCACCCCATCGGCTTCCATATCTTC
CAGGTCCCAGAGGGTGGAAGGAGCCAGGACGCACCCCCACTGCTGCTGCAGGAGCCGCTG
CTGAGCTGCGTGCCACATCGCTACGCCCAGGAGGTGAGCCGGCTCTGCCTCCTGCCTGCA
GGCACCTACAAGGTTGTGCCCTCCACCTACCTGCCGGACACAGAGGGGGCCTTCACAGTG
ACCATCGCAACCAGGATTGACAGGCCATCCATTCACAGCCAGGAGATGCTGGGCCAGTTC
CTCCAAGAGGTCTCCGTCATGGCAGTGATGAAAACCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2164
Start on reference sequence 146
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN10
Symbol Nomenclature : CAPN10
SYNONYM : CANP10; NIDDM1
Designation : calcium-activated neutral proteinase 10|calpain-10|calpain-like protease CAPN10
Full Nomenclature : calpain 10
GENEID : 11132
HGNC : 1477
MIM : 605286
Vega : OTTHUMG00000133358
Target GenBank: BC004260

 

Reference Sequence Alignment

  

HsCD00045536      ATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGAGCTGTTCCGGGACGCCGCCTTCCCC
NM_023083.3       ATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGAGCTGTTCCGGGACGCCGCCTTCCCC
                  ************************************************************

HsCD00045536      GCCGCGGACTCCTCGCTCTTCTGCGACTTGTCTACGCCGCTGGCCCAGTTCCGCGAGGAC
NM_023083.3       GCCGCGGACTCCTCGCTCTTCTGCGACTTGTCTACGCCGCTGGCCCAGTTCCGCGAGGAC
                  ************************************************************

HsCD00045536      ATCACGTGGAGGCGGCCCCAGGAGATTTGTGCCACACCCCGGCTGTTTCCAGATGACCCA
NM_023083.3       ATCACGTGGAGGCGGCCCCAGGAGATTTGTGCCACACCCCGGCTGTTTCCAGATGACCCA
                  ************************************************************

HsCD00045536      CGGGAAGGGCAGGTGAAGCAGGGGCTGCTGGGGGATTGCTGGTTCCTGTGTGCCTGCGCC
NM_023083.3       CGGGAAGGGCAGGTGAAGCAGGGGCTGCTGGGGGATTGCTGGTTCCTGTGTGCCTGCGCC
                  ************************************************************

HsCD00045536      GCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGTCATTCCTCCGGGACAGCCGAGCTGG
NM_023083.3       GCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGTCATTCCTCCGGGACAGCCGAGCTGG
                  ************************************************************

HsCD00045536      GCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCGCATTTGGCAGTTTGGACGCTGGGTG
NM_023083.3       GCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCGCATTTGGCAGTTTGGACGCTGGGTG
                  ************************************************************

HsCD00045536      GAGGTGACCACAGATGACCGCCTGCCGTGCCTTGCAGGGAGACTCTGTTTCTCCCGCTGC
NM_023083.3       GAGGTGACCACAGATGACCGCCTGCCGTGCCTTGCAGGGAGACTCTGTTTCTCCCGCTGC
                  ************************************************************

HsCD00045536      CAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGAAAAGGTCTACGCCAAGGTCCATGGG
NM_023083.3       CAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGAAAAGGTCTACGCCAAGGTCCATGGG
                  ************************************************************

HsCD00045536      TCCTACGAGCACCTGTGGGCCGGGCAGGTGGCGGATGCCCTGGTGGACCTGACCGGCGGC
NM_023083.3       TCCTACGAGCACCTGTGGGCCGGGCAGGTGGCGGATGCCCTGGTGGACCTGACCGGCGGC
                  ************************************************************

HsCD00045536      CTGGCAGAAAGATGGAACCTGAAGGGCGTAGCAGGAAGCGGAGGCCAGCAGGACAGGCCA
NM_023083.3       CTGGCAGAAAGATGGAACCTGAAGGGCGTAGCAGGAAGCGGAGGCCAGCAGGACAGGCCA
                  ************************************************************

HsCD00045536      GGCCGCTGGGAGCACAGGACTTGTCGGCAGCTGCTCCACCTGAAGGACCAGTGTCTGATC
NM_023083.3       GGCCGCTGGGAGCACAGGACTTGTCGGCAGCTGCTCCACCTGAAGGACCAGTGTCTGATC
                  ************************************************************

HsCD00045536      AGCTGCTGCGTGCTCAGCCCCAGAGCAGGTGCCCGGGAGCTGGGGGAGTTCCATGCCTTC
NM_023083.3       AGCTGCTGCGTGCTCAGCCCCAGAGCAGGTGCCCGGGAGCTGGGGGAGTTCCATGCCTTC
                  ************************************************************

HsCD00045536      ATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGCGGGCCAGTGCATCCTGCTGCTGCGG
NM_023083.3       ATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGCGGGCCAGTGCATCCTGCTGCTGCGG
                  ************************************************************

HsCD00045536      ATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGGGCTCTGGAGAGAGGGGGGTGAAGGG
NM_023083.3       ATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGGGCTCTGGAGAGAGGGGGGTGAAGGG
                  ************************************************************

HsCD00045536      TGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCTCCTGTCCCAGCTCCAGGAAGGGGAG
NM_023083.3       TGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCTCCTGTCCCAGCTCCAGGAAGGGGAG
                  ************************************************************

HsCD00045536      TTCTGGGTGGAGGAGGAGGAGTTCCTCAGGGAGTTTGACGAGCTCACCGTTGGCTACCCG
NM_023083.3       TTCTGGGTGGAGGAGGAGGAGTTCCTCAGGGAGTTTGACGAGCTCACCGTTGGCTACCCG
                  ************************************************************

HsCD00045536      GTCACGGAGGCCGGCCACCTGCAGAGCCTCTACACAGAGAGGCTGCTCTGCCATACGCGG
NM_023083.3       GTCACGGAGGCCGGCCACCTGCAGAGCCTCTACACAGAGAGGCTGCTCTGCCATACGCGG
                  ************************************************************

HsCD00045536      GCGCTGCCTGGGGCCTGGGTCAAGGGCCAGTCAGCAGGAGGCTGCCGGAACAACAGCGGC
NM_023083.3       GCGCTGCCTGGGGCCTGGGTCAAGGGCCAGTCAGCAGGAGGCTGCCGGAACAACAGCGGC
                  ************************************************************

HsCD00045536      TTTCCCAGCAACCCCAAATTCTGGCTGCGGGTCTCAGAACCGAGTGAGGTGTACATTGCC
NM_023083.3       TTTCCCAGCAACCCCAAATTCTGGCTGCGGGTCTCAGAACCGAGTGAGGTGTACATTGCC
                  ************************************************************

HsCD00045536      GTCCTGCAGAGATCCAGGCTGCACGCGGCGGACTGGGCAGGCCGGGCCCGGGCACTGGTG
NM_023083.3       GTCCTGCAGAGATCCAGGCTGCACGCGGCGGACTGGGCAGGCCGGGCCCGGGCACTGGTG
                  ************************************************************

HsCD00045536      GGTGACAGTCATACTTCGTGGAGCCCAGCGAGCATCCCGGGCAAGCACTACCAGGCTGTG
NM_023083.3       GGTGACAGTCATACTTCGTGGAGCCCAGCGAGCATCCCGGGCAAGCACTACCAGGCTGTG
                  ************************************************************

HsCD00045536      GGTCTGCACCTCTGGAAGGTAGAGAAGCGGCGGGTCAATCTGCCTAGGGTCCTGTCCATG
NM_023083.3       GGTCTGCACCTCTGGAAGGTAGAGAAGCGGCGGGTCAATCTGCCTAGGGTCCTGTCCATG
                  ************************************************************

HsCD00045536      CCCCCCGTGGCTGGCACCGCGTGCCATGCATACGACCGGGAGGTCCACCTGCGTTGTGAG
NM_023083.3       CCCCCCGTGGCTGGCACCGCGTGCCATGCATACGACCGGGAGGTCCACCTGCGTTGTGAG
                  ************************************************************

HsCD00045536      CTCTCACCGGGCTACTACCTGGCTGTCCCCAGCACCTTCCTGAAGGACGCGCCAGGGGAG
NM_023083.3       CTCTCACCGGGCTACTACCTGGCTGTCCCCAGCACCTTCCTGAAGGACGCGCCAGGGGAG
                  ************************************************************

HsCD00045536      TTCCTGCTCCGAGTCTTCTCTACCGGGCGAGTCTCCCTTAGCGCCATCAGGGCAGTGGCC
NM_023083.3       TTCCTGCTCCGAGTCTTCTCTACCGGGCGAGTCTCCCTTAGCGCCATCAGGGCAGTGGCC
                  ************************************************************

HsCD00045536      AAGAACACCACCCCCGGGGCAGCCCTGCCTGCGGGGGAGTGGGGGACCGTGCAGCTACGG
NM_023083.3       AAGAACACCACCCCCGGGGCAGCCCTGCCTGCGGGGGAGTGGGGGACCGTGCAGCTACGG
                  ************************************************************

HsCD00045536      GGTTCTTGGAGAGTCGGCCAGACGGCGGGGGGCAGCAGGAACTTTGCCTCATACCCCACC
NM_023083.3       GGTTCTTGGAGAGTCGGCCAGACGGCGGGGGGCAGCAGGAACTTTGCCTCATACCCCACC
                  ************************************************************

HsCD00045536      AACCCCTGCTTCCCCTTCTCGGTCCCCGAGGGCCCTGGCCCCCGCTGCGTCCGCATCACT
NM_023083.3       AACCCCTGCTTCCCCTTCTCGGTCCCCGAGGGCCCTGGCCCCCGCTGCGTCCGCATCACT
                  ************************************************************

HsCD00045536      CTGCATCAGCACTGCCGGCCCAGTGACACCGAGTTCCACCCCATCGGCTTCCATATCTTC
NM_023083.3       CTGCATCAGCACTGCCGGCCCAGTGACACCGAGTTCCACCCCATCGGCTTCCATATCTTC
                  ************************************************************

HsCD00045536      CAGGTCCCAGAGGGTGGAAGGAGCCAGGACGCACCCCCACTGCTGCTGCAGGAGCCGCTG
NM_023083.3       CAGGTCCCAGAGGGTGGAAGGAGCCAGGACGCACCCCCACTGCTGCTGCAGGAGCCGCTG
                  ************************************************************

HsCD00045536      CTGAGCTGCGTGCCACATCGCTACGCCCAGGAGGTGAGCCGGCTCTGCCTCCTGCCTGCA
NM_023083.3       CTGAGCTGCGTGCCACATCGCTACGCCCAGGAGGTGAGCCGGCTCTGCCTCCTGCCTGCG
                  ***********************************************************.

HsCD00045536      GGCACCTACAAGGTTGTGCCCTCCACCTACCTGCCGGACACAGAGGGGGCCTTCACAGTG
NM_023083.3       GGCACCTACAAGGTTGTGCCCTCCACCTACCTGCCGGACACAGAGGGGGCCTTCACAGTG
                  ************************************************************

HsCD00045536      ACCATCGCAACCAGGATTGACAGGCCATCCATTCACAGCCAGGAGATGCTGGGCCAGTTC
NM_023083.3       ACCATCGCAACCAGGATTGACAGGCCATCCATTCACAGCCAGGAGATGCTGGGCCAGTTC
                  ************************************************************

HsCD00045536      CTCCAAGAGGTCTCCGTCATGGCAGTGATGAAAACCTAG
NM_023083.3       CTCCAAGAGGTCTCCGTCATGGCAGTGATGAAAACCTAA
                  **************************************.

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
UniProt : Q9HC96
HPRD : 05595

Clone

Cloning Information : 194931
HIP Master Clone ID : 169642
Original Clone ID : FLH194931.01X

Organism


TAX_ID : 9606
Species Specific ID: 11132

Genome

Chromosome : 2
Map Location : 2q37.3
Ensembl : ENSG00000142330

Reagents

Labome : calpain-10-antibody