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HHIP (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HHIP
Gene Name:  hedgehog interacting protein
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH183739.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00045635

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2103nts         Open reading frame : 1 to 2103
Reference Sequence Alignment

ATGCTGAAGATGCTCTCCTTTAAGCTGCTGCTGCTGGCCGTGGCTCTGGGCTTCTTTGAA
GGAGATGCTAAGTTTGGGGAAAGAAACGAAGGGAGCGGAGCAAGGAGGAGAAGGTGCCTG
AATGGGAACCCCCCGAAGCGCCTGAAAAGGAGAGACAGGAGGATGATGTCCCAGCTGGAG
CTGCTGAGTGGGGGAGAGATGCTGTGCGGTGGCTTCTACCCTCGGCTGTCCTGCTGCCTG
CGGAGTGACAGCCCGGGGCTAGGGCGCCTGGAGAATAAGATATTTTCTGTTACCAACAAC
ACAGAATGTGGGAAGTTACTGGAGGAAATCAAATGTGCACTTTGCTCTCCACATTCTCAA
AGCCTGTTCCACTCACCTGAGAGAGAAGTCTTGGAAAGAGACCTAGTACTTCCTCTGCTC
TGCAAAGACTATTGCAAAGAATTCTTTTACACTTGCCGAGGCCATATTCCAGGTTTCCTT
CAAACAACTGCGGATGAGTTTTGCTTTTACTATGCAAGAAAAGATGGTGGGTTGTGCTTT
CCAGATTTTCCAAGAAAACAAGTCAGAGGACCAGCATCTAACTACTTGGACCAGATGGAA
GAATATGACAAAGTGGAAGAGATCAGCAGAAAGCACAAACACAACTGCTTCTGTATTCAG
GAGGTTGTGAGTGGGCTGCGGCAGCCCGTTGGTGCCCTGCATAGTGGGGATGGCTCGCAA
CGTCTCTTCATTCTGGAAAAAGAAGGTTATGTGAAGATACTTACCCCTGAAGGAGAAATT
TTCAAGGAGCCTTATTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAT
GAAAGAGGACTGCTAAGCCTCGCATTCCATCCCAATTACAAGAAAAATGGAAAGTTGTAT
GTGTCCTATACCACCAACCAAGAACGGTGGGCTATCGGGCCTCATGACCACATTCTTAGG
GTTGTGGAATACACAGTATCCAGAAAAAATCCACACCAAGTTGATTTGAGAACAGCCAGA
GTCTTTCTTGAAGTTGCAGAACTCCACAGAAAGCATCTGGGAGGACAACTGCTCTTTGGC
CCTGACGGCTTTTTGTACATCATTCTTGGTGATGGGATGATTACACTGGATGATATGGAA
GAAATGGATGGGTTAAGTGATTTCACAGGCTCAGTGCTACGGCTGGATGTGGACACAGAC
ATGTGCAACGTGCCTTATTCCATACCAAGGAGCAACCCACACTTCAACAGCACCAACCAG
CCCCCCGAAGTGTTTGCTCATGGGCTCCACGATCCAGGCAGATGTGCTGTGGATAGACAT
CCCACTGATATAAACATCAATTTAACGATACTGTGTTCAGACTCCAATGGAAAAAACAGA
TCATCAGCCAGAATTCTACAGATAATAAAGGGGAAAGATTATGAAAGTGAGCCATCACTT
TTAGAATTCAAGCCATTCAGTAATGGTCCTTTGGTTGGTGGATTTGTATACCGGGGCTGC
CAGTCAGAAAGATTGTATGGAAGCTACGTGTTTGGAGATCGTAATGGGAATTTCCTAACT
CTCCAGCAAAGTCCTGTGACAAAGCAGTGGCAAGAAAAACCACTCTGTCTCGGCACTAGT
GGGTCCTGTAGAGGCTACTTTTCCGGTCACATCTTGGGATTTGGAGAAGATGAACTAGGT
GAAGTTTACATTTTATCAAGCAGTAAAAGTATGACCCAGACTCACAATGGAAAACTCTAC
AAAATTGTAGATCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGCCACGGTACAACCT
GCACAGACACTGACTTCAGAGTGCTCCAGGCTCTGTCGAAACGGCTACTGCACCCCCACG
GGAAAGTGCTGCTGCAGTCCAGGCTGGGAGGGGGACTTCTGCAGAACTGCAAAATGTGAG
CCAGCATGTCGTCATGGAGGTGTCTGTGTTAGACCGAACAAGTGCCTCTGTAAAAAAGGA
TATCTTGGTCCTCAATGTGAACAAGTGGACAGAAACATCCGCAGAGTGACCAGGGCAGGT
ATTCTTGATCAGATCATTGACATGACATCTTACTTGCTGGATCTAACAAGTTACATTGTA
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2609
Start on reference sequence 507
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HHIP
Symbol Nomenclature : HHIP
SYNONYM : HIP
Designation : hedgehog-interacting protein
Full Nomenclature : hedgehog interacting protein
GENEID : 64399
Locus Tag : UNQ5825/PRO19644
HGNC : 14866
MIM : 606178
Target GenBank: BC025311

 

Reference Sequence Alignment

  

HsCD00045635      ATGCTGAAGATGCTCTCCTTTAAGCTGCTGCTGCTGGCCGTGGCTCTGGGCTTCTTTGAA
NM_022475.2       ATGCTGAAGATGCTCTCCTTTAAGCTGCTGCTGCTGGCCGTGGCTCTGGGCTTCTTTGAA
                  ************************************************************

HsCD00045635      GGAGATGCTAAGTTTGGGGAAAGAAACGAAGGGAGCGGAGCAAGGAGGAGAAGGTGCCTG
NM_022475.2       GGAGATGCTAAGTTTGGGGAAAGAAACGAAGGGAGCGGAGCAAGGAGGAGAAGGTGCCTG
                  ************************************************************

HsCD00045635      AATGGGAACCCCCCGAAGCGCCTGAAAAGGAGAGACAGGAGGATGATGTCCCAGCTGGAG
NM_022475.2       AATGGGAACCCCCCGAAGCGCCTGAAAAGGAGAGACAGGAGGATGATGTCCCAGCTGGAG
                  ************************************************************

HsCD00045635      CTGCTGAGTGGGGGAGAGATGCTGTGCGGTGGCTTCTACCCTCGGCTGTCCTGCTGCCTG
NM_022475.2       CTGCTGAGTGGGGGAGAGATGCTGTGCGGTGGCTTCTACCCTCGGCTGTCCTGCTGCCTG
                  ************************************************************

HsCD00045635      CGGAGTGACAGCCCGGGGCTAGGGCGCCTGGAGAATAAGATATTTTCTGTTACCAACAAC
NM_022475.2       CGGAGTGACAGCCCGGGGCTAGGGCGCCTGGAGAATAAGATATTTTCTGTTACCAACAAC
                  ************************************************************

HsCD00045635      ACAGAATGTGGGAAGTTACTGGAGGAAATCAAATGTGCACTTTGCTCTCCACATTCTCAA
NM_022475.2       ACAGAATGTGGGAAGTTACTGGAGGAAATCAAATGTGCACTTTGCTCTCCACATTCTCAA
                  ************************************************************

HsCD00045635      AGCCTGTTCCACTCACCTGAGAGAGAAGTCTTGGAAAGAGACCTAGTACTTCCTCTGCTC
NM_022475.2       AGCCTGTTCCACTCACCTGAGAGAGAAGTCTTGGAAAGAGACCTAGTACTTCCTCTGCTC
                  ************************************************************

HsCD00045635      TGCAAAGACTATTGCAAAGAATTCTTTTACACTTGCCGAGGCCATATTCCAGGTTTCCTT
NM_022475.2       TGCAAAGACTATTGCAAAGAATTCTTTTACACTTGCCGAGGCCATATTCCAGGTTTCCTT
                  ************************************************************

HsCD00045635      CAAACAACTGCGGATGAGTTTTGCTTTTACTATGCAAGAAAAGATGGTGGGTTGTGCTTT
NM_022475.2       CAAACAACTGCGGATGAGTTTTGCTTTTACTATGCAAGAAAAGATGGTGGGTTGTGCTTT
                  ************************************************************

HsCD00045635      CCAGATTTTCCAAGAAAACAAGTCAGAGGACCAGCATCTAACTACTTGGACCAGATGGAA
NM_022475.2       CCAGATTTTCCAAGAAAACAAGTCAGAGGACCAGCATCTAACTACTTGGACCAGATGGAA
                  ************************************************************

HsCD00045635      GAATATGACAAAGTGGAAGAGATCAGCAGAAAGCACAAACACAACTGCTTCTGTATTCAG
NM_022475.2       GAATATGACAAAGTGGAAGAGATCAGCAGAAAGCACAAACACAACTGCTTCTGTATTCAG
                  ************************************************************

HsCD00045635      GAGGTTGTGAGTGGGCTGCGGCAGCCCGTTGGTGCCCTGCATAGTGGGGATGGCTCGCAA
NM_022475.2       GAGGTTGTGAGTGGGCTGCGGCAGCCCGTTGGTGCCCTGCATAGTGGGGATGGCTCGCAA
                  ************************************************************

HsCD00045635      CGTCTCTTCATTCTGGAAAAAGAAGGTTATGTGAAGATACTTACCCCTGAAGGAGAAATT
NM_022475.2       CGTCTCTTCATTCTGGAAAAAGAAGGTTATGTGAAGATACTTACCCCTGAAGGAGAAATT
                  ************************************************************

HsCD00045635      TTCAAGGAGCCTTATTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAT
NM_022475.2       TTCAAGGAGCCTTATTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAT
                  ************************************************************

HsCD00045635      GAAAGAGGACTGCTAAGCCTCGCATTCCATCCCAATTACAAGAAAAATGGAAAGTTGTAT
NM_022475.2       GAAAGAGGACTGCTAAGCCTCGCATTCCATCCCAATTACAAGAAAAATGGAAAGTTGTAT
                  ************************************************************

HsCD00045635      GTGTCCTATACCACCAACCAAGAACGGTGGGCTATCGGGCCTCATGACCACATTCTTAGG
NM_022475.2       GTGTCCTATACCACCAACCAAGAACGGTGGGCTATCGGGCCTCATGACCACATTCTTAGG
                  ************************************************************

HsCD00045635      GTTGTGGAATACACAGTATCCAGAAAAAATCCACACCAAGTTGATTTGAGAACAGCCAGA
NM_022475.2       GTTGTGGAATACACAGTATCCAGAAAAAATCCACACCAAGTTGATTTGAGAACAGCCAGA
                  ************************************************************

HsCD00045635      GTCTTTCTTGAAGTTGCAGAACTCCACAGAAAGCATCTGGGAGGACAACTGCTCTTTGGC
NM_022475.2       GTCTTTCTTGAAGTTGCAGAACTCCACAGAAAGCATCTGGGAGGACAACTGCTCTTTGGC
                  ************************************************************

HsCD00045635      CCTGACGGCTTTTTGTACATCATTCTTGGTGATGGGATGATTACACTGGATGATATGGAA
NM_022475.2       CCTGACGGCTTTTTGTACATCATTCTTGGTGATGGGATGATTACACTGGATGATATGGAA
                  ************************************************************

HsCD00045635      GAAATGGATGGGTTAAGTGATTTCACAGGCTCAGTGCTACGGCTGGATGTGGACACAGAC
NM_022475.2       GAAATGGATGGGTTAAGTGATTTCACAGGCTCAGTGCTACGGCTGGATGTGGACACAGAC
                  ************************************************************

HsCD00045635      ATGTGCAACGTGCCTTATTCCATACCAAGGAGCAACCCACACTTCAACAGCACCAACCAG
NM_022475.2       ATGTGCAACGTGCCTTATTCCATACCAAGGAGCAACCCACACTTCAACAGCACCAACCAG
                  ************************************************************

HsCD00045635      CCCCCCGAAGTGTTTGCTCATGGGCTCCACGATCCAGGCAGATGTGCTGTGGATAGACAT
NM_022475.2       CCCCCCGAAGTGTTTGCTCATGGGCTCCACGATCCAGGCAGATGTGCTGTGGATAGACAT
                  ************************************************************

HsCD00045635      CCCACTGATATAAACATCAATTTAACGATACTGTGTTCAGACTCCAATGGAAAAAACAGA
NM_022475.2       CCCACTGATATAAACATCAATTTAACGATACTGTGTTCAGACTCCAATGGAAAAAACAGA
                  ************************************************************

HsCD00045635      TCATCAGCCAGAATTCTACAGATAATAAAGGGGAAAGATTATGAAAGTGAGCCATCACTT
NM_022475.2       TCATCAGCCAGAATTCTACAGATAATAAAGGGGAAAGATTATGAAAGTGAGCCATCACTT
                  ************************************************************

HsCD00045635      TTAGAATTCAAGCCATTCAGTAATGGTCCTTTGGTTGGTGGATTTGTATACCGGGGCTGC
NM_022475.2       TTAGAATTCAAGCCATTCAGTAATGGTCCTTTGGTTGGTGGATTTGTATACCGGGGCTGC
                  ************************************************************

HsCD00045635      CAGTCAGAAAGATTGTATGGAAGCTACGTGTTTGGAGATCGTAATGGGAATTTCCTAACT
NM_022475.2       CAGTCAGAAAGATTGTATGGAAGCTACGTGTTTGGAGATCGTAATGGGAATTTCCTAACT
                  ************************************************************

HsCD00045635      CTCCAGCAAAGTCCTGTGACAAAGCAGTGGCAAGAAAAACCACTCTGTCTCGGCACTAGT
NM_022475.2       CTCCAGCAAAGTCCTGTGACAAAGCAGTGGCAAGAAAAACCACTCTGTCTCGGCACTAGT
                  ************************************************************

HsCD00045635      GGGTCCTGTAGAGGCTACTTTTCCGGTCACATCTTGGGATTTGGAGAAGATGAACTAGGT
NM_022475.2       GGGTCCTGTAGAGGCTACTTTTCCGGTCACATCTTGGGATTTGGAGAAGATGAACTAGGT
                  ************************************************************

HsCD00045635      GAAGTTTACATTTTATCAAGCAGTAAAAGTATGACCCAGACTCACAATGGAAAACTCTAC
NM_022475.2       GAAGTTTACATTTTATCAAGCAGTAAAAGTATGACCCAGACTCACAATGGAAAACTCTAC
                  ************************************************************

HsCD00045635      AAAATTGTAGATCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGCCACGGTACAACCT
NM_022475.2       AAAATTGTAGATCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGCCACGGTACAACCT
                  ************************************************************

HsCD00045635      GCACAGACACTGACTTCAGAGTGCTCCAGGCTCTGTCGAAACGGCTACTGCACCCCCACG
NM_022475.2       GCACAGACACTGACTTCAGAGTGCTCCAGGCTCTGTCGAAACGGCTACTGCACCCCCACG
                  ************************************************************

HsCD00045635      GGAAAGTGCTGCTGCAGTCCAGGCTGGGAGGGGGACTTCTGCAGAACTGCAAAATGTGAG
NM_022475.2       GGAAAGTGCTGCTGCAGTCCAGGCTGGGAGGGGGACTTCTGCAGAACTGCAAAATGTGAG
                  ************************************************************

HsCD00045635      CCAGCATGTCGTCATGGAGGTGTCTGTGTTAGACCGAACAAGTGCCTCTGTAAAAAAGGA
NM_022475.2       CCAGCATGTCGTCATGGAGGTGTCTGTGTTAGACCGAACAAGTGCCTCTGTAAAAAAGGA
                  ************************************************************

HsCD00045635      TATCTTGGTCCTCAATGTGAACAAGTGGACAGAAACATCCGCAGAGTGACCAGGGCAGGT
NM_022475.2       TATCTTGGTCCTCAATGTGAACAAGTGGACAGAAACATCCGCAGAGTGACCAGGGCAGGT
                  ************************************************************

HsCD00045635      ATTCTTGATCAGATCATTGACATGACATCTTACTTGCTGGATCTAACAAGTTACATTGTA
NM_022475.2       ATTCTTGATCAGATCATTGACATGACATCTTACTTGCTGGATCTAACAAGTTACATTGTA
                  ************************************************************

HsCD00045635      TAG
NM_022475.2       TAG
                  ***

Pathways

NCI : Signaling events mediated by the Hedgehog family

Protein

SMART domain : EGF : Epidermal growth factor-like domain.
UniProt : Q96QV1
HPRD : 06937

Clone

Cloning Information : 183739
HIP Master Clone ID : 158683
Original Clone ID : FLH183739.01X

Organism


TAX_ID : 9606
Species Specific ID: 64399

Genome

Chromosome : 4
Map Location : 4q28-q32