HEPH (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  HEPH
Gene Name:  hephaestin
Original Clone ID:
FLH184601.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00045645
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3477nts
Open reading frame: 1 to 3477

ATGGAGTCAGGCCACCTCCTCTGGGCTCTGCTGTTCATGCAGTCCTTGTGGCCTCAACTG
ACTGATGGAGCCACTCGAGTCTACTACCTGGGCATCCGGGATGTGCAGTGGAACTATGCT
CCCAAGGGAAGAAATGTCATCACGAACCAGCCTCTGGACAGTGACATAGTGGCTTCCAGC
TTCTTAAAGTCTGACAAGAACCGGATAGGGGGAACCTACAAGAAGACCATCTATAAAGAA
TACAAGGATGACTCATACACAGATGAAGTGGCCCAGCCTGCCTGGTTGGGCTTCCTGGGG
CCAGTGTTGCAGGCTGAAGTGGGGGATGTCATTCTTATTCACCTGAAGAATTTTGCCACT
CGTCCCTATACCATCCACCCTCATGGTGTCTTCTACGAGAAGGACTCTGAAGGTTCCCTA
TACCCAGATGGCTCCTCTGGGCCACTGAAAGCTGATGACTCTGTTCCCCCGGGGGGCAGC
CATATCTACAACTGGACCATTCCAGAAGGCCATGCACCCACCGATGCTGACCCAGCGTGC
CTCACCTGGATCTACCATTCTCATGTAGATGCTCCACGAGACATTGCAACTGGCCTAATT
GGGCCTCTCATCACCTGTAAAAGAGGAGCCCTGGATGGGAACTCCCCTCCTCAACGCCAG
GATGTAGACCATGATTTCTTCCTCCTCTTCAGTGTGGTAGATGAGAACCTCAGCTGGCAT
CTCAATGAGAACATTGCCACTTACTGCTCAGATCCTGCTTCAGTGGACAAAGAAGATGAG
ACATTTCAGGAGAGCAATAGGATGCATGCAATCAATGGCTTTGTTTTTGGGAATTTACCT
GAGCTGAACATGTGTGCACAGAAACGTGTGGCCTGGCACTTGTTTGGCATGGGCAATGAA
ATTGATGTCCACACAGCATTTTTCCATGGACAGATGCTGACTACCCGTGGACACCACACT
GATGTGGCTAACATCTTTCCAGCCACCTTTGTGACTGCTGAGATGGTGCCCTGGGAACCT
GGTACCTGGTTAATTAGCTGCCAAGTGAACAGTCACTTTCGAGATGGCATGCAGGCACTC
TACAAGGTCAAGTCTTGCTCCATGGCCCCTCCTGTGGACCTGCTCACAGGCAAAGTTCGA
CAGTACTTCATTGAGGCCCATGAGATTCAATGGGACTATGGCCCGATGGGGCATGATGGG
AGTACTGGGAAGAATTTGAGAGAGCCAGGCAGTATCTCAGATAAGTTTTTCCAGAAGAGC
TCCAGCCGAATTGGGGGCACTTACTGGAAAGTGCGATATGAAGCCTTTCAAGATGAGACA
TTCCAAGAGAAGATGCATTTGGAGGAAGATAGGCATCTTGGAATCCTGGGGCCAGTGATC
CGGGCTGAGGTGGGTGACACCATTCAGGTGGTCTTCTACAACCGTGCCTCCCAGCCATTC
AGCATGCAGCCCCATGGGGTCTTTTATGAGAAAGACTATGAAGGCACTGTGTACAATGAT
GGCTCATCTTACCCTGGCTTGGTTGCCAAGCCCTTTGAGAAAGTAACATACCGCTGGACA
GTCCCCCCTCATGCCGGTCCCACTGCTCAGGATCCTGCTTGTCTCACTTGGATGTACTTC
TCTGCTGCAGATCCCATAAGAGACACAAATTCTGGCCTGGTGGGCCCGCTGCTGGTGTGC
AGGGCTGGTGCCTTGGGTGCAGATGGCAAGCAGAAAGGGGTGGATAAAGAATTCTTTCTT
CTCTTCACTGTGTTGGATGAGAACAAGAGCTGGTACAGCAATGCCAATCAAGCAGCTGCT
ATGTTGGATTTCCGACTGCTTTCAGAGGATATTGAGGGCTTCCAAGACTCCAATCGGATG
CATGCCATTAATGGGTTTCTGTTCTCTAACCTGCCCAGGCTGGACATGTGCAAGGGTGAC
ACAGTGGCCTGGCACCTGCTCGGCCTGGGCACAGAGACTGATGTGCATGGAGTCATGTTC
CAGGGCAACACTGTGCAGCTTCAGGGCATGAGGAAGGGTGCAGCTATGCTCTTTCCTCAT
ACCTTTGTCATGGCCATCATGCAGCCTGACAACCTTGGGACATTTGAGATTTATTGCCAG
GCAGGCAGCCATCGAGAAGCAGGGATGAGGGCAATCTATAATGTCTCCCAGTGTCCTGGC
CACCAAGCCACCCCTCGCCAACGCTACCAAGCTGCAAGAATCTACTATATCATGGCAGAA
GAAGTAGAGTGGGACTATTGCCCTGACCGGAGCTGGGAACGGGAATGGCACAACCAGTCT
GAGAAGGACAGTTATGGTTACATTTTCCTGAGCAACAAGGATGGGCTCCTGGGTTCCAGA
TACAAGAAAGCTGTATTCAGGGAATACACTGATGGTACATTCAGGATCCCTCGGCCAAGG
ACTGGACCAGAAGAACACTTGGGAATCTTGGGTCCACTTATCAAAGGTGAAGTTGGTGAT
ATCCTGACTGTGGTATTCAAGAATAATGCCAGCCGCCCCTACTCTGTGCATGCTCATGGA
GTGCTAGAATCTACTACTGTCTGGCCACTGGCTGCTGAGCCTGGTGAGGTGGTCACTTAT
CAGTGGAACATCCCAGAGAGGTCTGGCCCTGGGCCCAATGACTCTGCTTGTGTTTCCTGG
ATCTATTATTCTGCAGTGGATCCCATCAAGGACATGTATAGTGGCCTGGTGGGGCCCTTG
GCTATCTGCCAAAAGGGCATCCTGGAGCCCCATGGAGGACGGAGTGACATGGATCGGGAA
TTTGCATTGTTGTTCTTGATTTTTGATGAAAATAAGTCTTGGTATTTGGAGGAAAATGTG
GCAACCCATGGGTCCCAGGATCCAGGCAGTATTAACCTACAGGATGAAACTTTCTTGGAG
AGCAATAAAATGCATGCAATCAATGGGAAACTCTATGCCAACCTTAGGGGTCTTACCATG
TACCAAGGAGAACGAGTGGCCTGGTACATGCTGGCCATGGGCCAAGATGTGGATCTACAC
ACCATCCACTTTCATGCAGAGAGCTTCCTCTATCGGAATGGCGAGAACTACCGGGCAGAT
GTGGTGGATCTGTTCCCAGGGACTTTTGAGGTTGTGGAGATGGTGGCCAGCAACCCTGGG
ACATGGCTGATGCACTGCCATGTGACTGACCATGTCCATGCTGGCATGGAGACCCTCTTC
ACTGTTTTTTCTCGAACAGAACACTTAAGCCCTCTCACCGTCATCACCAAAGAGACTGAA
AAAGCAGTGCCCCCCAGAGACATTGAAGAAGGCAATGTGAAGATGCTGGGCATGCAGATC
CCCATAAAGAATGTTGAGATGCTGGCCTCTGTTTTGGTTGCCATTAGTGTCACCCTTCTG
CTCGTTGTTCTGGCTCTTGGTGGAGTGGTTTGGTACCAACATCGACAGAGAAAGCTACGA
CGCAATAGGAGGTCCATCCTGGATGACAGCTTCAAGCTTCTGTCTTTCAAACAGTAG

Protein sequence predicted based on nucleotide sequence

MESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASS
FLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFAT
RPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPAC
LTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQRQDVDHDFFLLFSVVDENLSWH
LNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLPELNMCAQKRVAWHLFGMGNE
IDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQAL
YKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKS
SSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPF
SMQPHGVFYEKDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYF
SAADPIRDTNSGLVGPLLVCRAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAA
MLDFRLLSEDIEGFQDSNRMHAINGFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMF
QGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPG
HQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSR
YKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHG
VLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPL
AICQKGILEPHGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLE
SNKMHAINGKLYANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRAD
VVDLFPGTFEVVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETE
KAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLR
RNRRSILDDSFKLLSFKQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3546
Start on reference sequence 70
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable