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KLHL2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL2
Gene Name:  kelch-like family member 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH185732.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00045655

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1782nts         Open reading frame : 1 to 1782
Reference Sequence Alignment

ATGGAGACGCCGCCGCTGCCTCCCGCATGCACAAAGCAGGGTCATCAGAAGCCTCTCGAT
TCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCTTGGCATATGAAG
AAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGCGATGTCACAATT
GTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCCTGTAGTCCTTAT
TTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGAGTTAGAATAAAA
GAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACTGCAGAAATTCAG
GTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTACAGTTACAGGAT
GTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTCAACTGCTTAGGA
ATCCGGGCTTTTGCTGATATGCATGCATGTACTGACCTTCTGAACAAGGCCAACACCTAT
GCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAATCTTGGCATCGAA
CAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAAGAGAAGGTATTT
GAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAGTTTATGGCCCGA
CTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTTCAGAGGGTTGAA
GAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATTGAAGCAATGAAG
TACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGGACCCGGCTGAGG
ACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCACCAAAGGCTATC
CGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTAGCAGAGTTGCCT
TCCAGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTTGCTGTTGGTGGC
TTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTGAAGGACCAGTGG
ACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCTGTGTTAAATGGA
TTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCTGTGGAAGCATAC
AACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGGAGGAGCAGTGTT
GGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGATGTAGCATCACGT
CAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGGACCTATATAGCA
GAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAATTTATTGTATGCT
GTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATATGATCCCACCACT
AACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCAGGAGTTTGTGCA
GTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAACTTGGCGTCAGTA
GAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGTATGAGCACAGGG
AGAAGTTATGCAGGGGTCACAGTTATTGATAAACGATTATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2023
Start on reference sequence 242
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL2
Symbol Nomenclature : KLHL2
SYNONYM : ABP-KELCH; MAV; MAYVEN
Designation : actin-binding protein Mayven|kelch|kelch-like 2, Mayven|kelch-like protein 2
Full Nomenclature : kelch-like family member 2
GENEID : 11275
HGNC : 6353
MIM : 605774
Vega : OTTHUMG00000161294
Target GenBank: BC022503

 

Reference Sequence Alignment

  

HsCD00045655        ------------ATGGAGACGCCGCCGCTGCCTCCCGCATGCACAAAGCAGGGTCATCAG
NM_001161521.1      ATGGTATGGTTGGAAGCACGGCCCCAGATTCTCTTTGTATGCACAAAGCAGGGTCATCAG
NM_007246.3         ------------ATGGAGACGCCGCCGCTGCCTCCCGCATGCACAAAGCAGGGTCATCAG
                                .:.*... *** *.*.* *     * **********************

HsCD00045655        AAGCCTCTCGATTCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCT
NM_001161521.1      AAGCCTCTCGATTCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCT
NM_007246.3         AAGCCTCTCGATTCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCT
                    ************************************************************

HsCD00045655        TGGCATATGAAGAAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGC
NM_001161521.1      TGGCATATGAAGAAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGC
NM_007246.3         TGGCATATGAAGAAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGC
                    ************************************************************

HsCD00045655        GATGTCACAATTGTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCC
NM_001161521.1      GATGTCACAATTGTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCC
NM_007246.3         GATGTCACAATTGTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCC
                    ************************************************************

HsCD00045655        TGTAGTCCTTATTTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGA
NM_001161521.1      TGTAGTCCTTATTTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGA
NM_007246.3         TGTAGTCCTTATTTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGA
                    ************************************************************

HsCD00045655        GTTAGAATAAAAGAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACT
NM_001161521.1      GTTAGAATAAAAGAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACT
NM_007246.3         GTTAGAATAAAAGAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACT
                    ************************************************************

HsCD00045655        GCAGAAATTCAGGTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTA
NM_001161521.1      GCAGAAATTCAGGTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTA
NM_007246.3         GCAGAAATTCAGGTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTA
                    ************************************************************

HsCD00045655        CAGTTACAGGATGTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTC
NM_001161521.1      CAGTTACAGGATGTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTC
NM_007246.3         CAGTTACAGGATGTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTC
                    ************************************************************

HsCD00045655        AACTGCTTAGGAATCCGGGCTTTTGCTGATATGCATGCATGTACTGACCTTCTGAACAAG
NM_001161521.1      AACTGCTTAGGAATCCGGGCTTTTGCTGATATGCATGCATGTACCGACCTTCTGAACAAG
NM_007246.3         AACTGCTTAGGAATCCGGGCTTTTGCTGATATGCATGCATGTACCGACCTTCTGAACAAG
                    ******************************************** ***************

HsCD00045655        GCCAACACCTATGCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAAT
NM_001161521.1      GCCAACACCTATGCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAAT
NM_007246.3         GCCAACACCTATGCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAAT
                    ************************************************************

HsCD00045655        CTTGGCATCGAACAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAA
NM_001161521.1      CTTGGCATCGAACAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAA
NM_007246.3         CTTGGCATCGAACAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAA
                    ************************************************************

HsCD00045655        GAGAAGGTATTTGAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAG
NM_001161521.1      GAGAAGGTATTTGAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAG
NM_007246.3         GAGAAGGTATTTGAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAG
                    ************************************************************

HsCD00045655        TTTATGGCCCGACTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTT
NM_001161521.1      TTTATGGCCCGACTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTT
NM_007246.3         TTTATGGCCCGACTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTT
                    ************************************************************

HsCD00045655        CAGAGGGTTGAAGAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATT
NM_001161521.1      CAGAGGGTTGAAGAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATT
NM_007246.3         CAGAGGGTTGAAGAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATT
                    ************************************************************

HsCD00045655        GAAGCAATGAAGTACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGG
NM_001161521.1      GAAGCAATGAAGTACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGG
NM_007246.3         GAAGCAATGAAGTACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGG
                    ************************************************************

HsCD00045655        ACCCGGCTGAGGACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCA
NM_001161521.1      ACCCGGCTGAGGACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCA
NM_007246.3         ACCCGGCTGAGGACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCA
                    ************************************************************

HsCD00045655        CCAAAGGCTATCCGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTA
NM_001161521.1      CCAAAGGCTATCCGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTA
NM_007246.3         CCAAAGGCTATCCGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTA
                    ************************************************************

HsCD00045655        GCAGAGTTGCCTTCCAGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTT
NM_001161521.1      GCAGAGTTGCCTTCCAGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTT
NM_007246.3         GCAGAGTTGCCTTCCAGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTT
                    ************************************************************

HsCD00045655        GCTGTTGGTGGCTTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTG
NM_001161521.1      GCTGTTGGTGGCTTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTG
NM_007246.3         GCTGTTGGTGGCTTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTG
                    ************************************************************

HsCD00045655        AAGGACCAGTGGACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCT
NM_001161521.1      AAGGACCAGTGGACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCT
NM_007246.3         AAGGACCAGTGGACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCT
                    ************************************************************

HsCD00045655        GTGTTAAATGGATTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCT
NM_001161521.1      GTGTTAAATGGATTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCT
NM_007246.3         GTGTTAAATGGATTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCT
                    ************************************************************

HsCD00045655        GTGGAAGCATACAACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGG
NM_001161521.1      GTGGAAGCATACAACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGG
NM_007246.3         GTGGAAGCATACAACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGG
                    ************************************************************

HsCD00045655        AGGAGCAGTGTTGGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGAT
NM_001161521.1      AGGAGCAGTGTTGGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGAT
NM_007246.3         AGGAGCAGTGTTGGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGAT
                    ************************************************************

HsCD00045655        GTAGCATCACGTCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGG
NM_001161521.1      GGAGCATCACGTCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGG
NM_007246.3         GGAGCATCACGTCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGG
                    * **********************************************************

HsCD00045655        ACCTATATAGCAGAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAAT
NM_001161521.1      ACCTATATAGCAGAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAAT
NM_007246.3         ACCTATATAGCAGAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAAT
                    ************************************************************

HsCD00045655        TTATTGTATGCTGTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATAT
NM_001161521.1      TTATTGTATGCTGTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATAT
NM_007246.3         TTATTGTATGCTGTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATAT
                    ************************************************************

HsCD00045655        GATCCCACCACTAACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCA
NM_001161521.1      GATCCCACCACTAACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCA
NM_007246.3         GATCCCACCACTAACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCA
                    ************************************************************

HsCD00045655        GGAGTTTGTGCAGTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAAC
NM_001161521.1      GGAGTTTGTGCAGTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAAC
NM_007246.3         GGAGTTTGTGCAGTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAAC
                    ************************************************************

HsCD00045655        TTGGCGTCAGTAGAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGT
NM_001161521.1      TTGGCGTCAGTAGAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGT
NM_007246.3         TTGGCGTCAGTAGAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGT
                    ************************************************************

HsCD00045655        ATGAGCACAGGGAGAAGTTATGCAGGGGTCACAGTTATTGATAAACGATTATAG
NM_001161521.1      ATGAGCACAGGGAGAAGTTATGCAGGGGTCACAGTTATTGATAAACCATTATGA
NM_007246.3         ATGAGCACAGGGAGAAGTTATGCAGGGGTCACAGTTATTGATAAACCATTATGA
                    ********************************************** *****..

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
UniProt : O95198
UniProt : B4DFH7
HPRD : 09311

Clone

Cloning Information : 185732
HIP Master Clone ID : 160676
Original Clone ID : FLH185732.01X

Organism


TAX_ID : 9606
Species Specific ID: 11275

Genome

Chromosome : 4
Map Location : 4q21.2
Ensembl : ENSG00000109466

Reagents

Labome : KLHL2-antibody