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VAT1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  VAT1
Gene Name:  vesicle amine transport 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH013450.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00004822

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1182nts         Open reading frame : 1 to 1182
Reference Sequence Alignment

ATGTCCGACGAGAGAGAGGTAGCCGAGGCAGCGACCGGGGAAGACGCCTCTTCGCCGCCT
CCGAAAACCGAGGCAGCGAGCGACCCCCAGCATCCCGCGGCCTCCGAAGGGGCCGCCGCC
GCCGCCGCCTCGCCGCCACTGCTGCGCTGCCTAGTGCTCACCGGCTTTGGAGGCTACGAC
AAGGTGAAGCTGCAGAGCCGGCCGGCAGCGCCCCCGGCCCCTGGGCCCGGCCAGCTGACG
CTGCGTCTGCGGGCCTGCGGGCTCAACTTCGCAGACCTCATGGCTAGGCAGGGGCTGTAC
GACCGTCTCCCGCCTCTGCCTGTCACTCCGGGCATGGAGGGCGCGGGTGTTGTGATCGCA
GTGGGCGAGGGAGTCAGCGACCGCAAGGCAGGAGACCGGGTGATGGTGTTGAACCGGTCA
GGGATGTGGCAGGAAGAGGTGACTGTGCCCTCGGTCCAGACCTTCCTGATTCCTGAGGCC
ATGACCTTTGAGGAAGCTGCTGCCTTGCTCGTCAATTACATTACAGCCTACATGGTCCTC
TTTGACTTCGGCAACCTACAGCCTGGCCACAGCGTCTTGGTACACATGGCTGCAGGGGGT
GTGGGTATGGCTGCCGTGCAGCTGTGCCGTACAGTGGAGAATGTGACAGTGTTCGGAACG
GCCTCGGCCAGCAAGCACGAGGCACTGAAGGAGAATGGGGTCACACATCCCATCGACTAT
CACACGACTGACTACGTGGATGAGATCAAGAAGATTTCCCCTAAAGGAGTGGACATTGTC
ATGGACCCTCTGGGTGGGTCAGATACTGCCAAGGGCTACAACCTCCTGAAACCCATGGGC
AAAGTCGTCACCTATGGAATGGCCAACCTGCTGACGGGCCCCAAACGGAACCTGATGGCC
CTGGCCCGGACATGGTGGAATCAGTTCAGCGTGACAGCTCTGCAGCTGCTGCAGGCCAAC
CGGGCTGTGTGTGGCTTCCACCTGGGCTACCTGGATGGTGAGGTGGAGCTGGTCAGTGGT
GTGGTGGCCCGCCTCCTGGCTCTGTACAACCAGGGCCACATCAAGCCCCACATTGACTCA
GTCTGGCCCTTCGAGAAGGTGGCTGATGCCATGAAACAGATGCAGGAGAAGAAGAATGTG
GGCAAGGTCCTCCTGGTTCCAGGGCCAGAGAAGGAGAACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1217
Start on reference sequence 36
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : VAT1
Symbol Nomenclature : VAT1
SYNONYM : VATI
Designation : membrane protein of cholinergic synaptic vesicles|synaptic vesicle membrane protein VAT-1 homolog|vesicle amine transport protein 1 homolog (T. californica)
Full Nomenclature : vesicle amine transport 1
GENEID : 10493
GI : 61362825
GenBank Accession : AY890346
HGNC : 16919
MIM : 604631
Vega : OTTHUMG00000180816
Target GenBank: BC014279

 

Reference Sequence Alignment

  

HsCD00004822      ATGTCCGACGAGAGAGAGGTAGCCGAGGCAGCGACCGGGGAAGACGCCTCTTCGCCGCCT
NM_006373.3       ATGTCCGACGAGAGAGAGGTAGCCGAGGCAGCGACCGGGGAAGACGCCTCTTCGCCGCCT
                  ************************************************************

HsCD00004822      CCGAAAACCGAGGCAGCGAGCGACCCCCAGCATCCCGCGGCCTCCGAAGGGGCCGCCGCC
NM_006373.3       CCGAAAACCGAGGCAGCGAGCGACCCCCAGCATCCCGCGGCCTCCGAAGGGGCCGCCGCC
                  ************************************************************

HsCD00004822      GCCGCCGCCTCGCCGCCACTGCTGCGCTGCCTAGTGCTCACCGGCTTTGGAGGCTACGAC
NM_006373.3       GCCGCCGCCTCGCCGCCACTGCTGCGCTGCCTAGTGCTCACCGGCTTTGGAGGCTACGAC
                  ************************************************************

HsCD00004822      AAGGTGAAGCTGCAGAGCCGGCCGGCAGCGCCCCCGGCCCCTGGGCCCGGCCAGCTGACG
NM_006373.3       AAGGTGAAGCTGCAGAGCCGGCCGGCAGCGCCCCCGGCCCCTGGGCCCGGCCAGCTGACG
                  ************************************************************

HsCD00004822      CTGCGTCTGCGGGCCTGCGGGCTCAACTTCGCAGACCTCATGGCTAGGCAGGGGCTGTAC
NM_006373.3       CTGCGTCTGCGGGCCTGCGGGCTCAACTTCGCAGACCTCATGGCTAGGCAGGGGCTGTAC
                  ************************************************************

HsCD00004822      GACCGTCTCCCGCCTCTGCCTGTCACTCCGGGCATGGAGGGCGCGGGTGTTGTGATCGCA
NM_006373.3       GACCGTCTCCCGCCTCTGCCTGTCACTCCGGGCATGGAGGGCGCGGGTGTTGTGATCGCA
                  ************************************************************

HsCD00004822      GTGGGCGAGGGAGTCAGCGACCGCAAGGCAGGAGACCGGGTGATGGTGTTGAACCGGTCA
NM_006373.3       GTGGGCGAGGGAGTCAGCGACCGCAAGGCAGGAGACCGGGTGATGGTGTTGAACCGGTCA
                  ************************************************************

HsCD00004822      GGGATGTGGCAGGAAGAGGTGACTGTGCCCTCGGTCCAGACCTTCCTGATTCCTGAGGCC
NM_006373.3       GGGATGTGGCAGGAAGAGGTGACTGTGCCCTCGGTCCAGACCTTCCTGATTCCTGAGGCC
                  ************************************************************

HsCD00004822      ATGACCTTTGAGGAAGCTGCTGCCTTGCTCGTCAATTACATTACAGCCTACATGGTCCTC
NM_006373.3       ATGACCTTTGAGGAAGCTGCTGCCTTGCTCGTCAATTACATTACAGCCTACATGGTCCTC
                  ************************************************************

HsCD00004822      TTTGACTTCGGCAACCTACAGCCTGGCCACAGCGTCTTGGTACACATGGCTGCAGGGGGT
NM_006373.3       TTTGACTTCGGCAACCTACAGCCTGGCCACAGCGTCTTGGTACACATGGCTGCAGGGGGT
                  ************************************************************

HsCD00004822      GTGGGTATGGCTGCCGTGCAGCTGTGCCGTACAGTGGAGAATGTGACAGTGTTCGGAACG
NM_006373.3       GTGGGTATGGCTGCCGTGCAGCTGTGCCGTACAGTGGAGAATGTGACAGTGTTCGGAACG
                  ************************************************************

HsCD00004822      GCCTCGGCCAGCAAGCACGAGGCACTGAAGGAGAATGGGGTCACACATCCCATCGACTAT
NM_006373.3       GCCTCGGCCAGCAAGCACGAGGCACTGAAGGAGAATGGGGTCACACATCCCATCGACTAT
                  ************************************************************

HsCD00004822      CACACGACTGACTACGTGGATGAGATCAAGAAGATTTCCCCTAAAGGAGTGGACATTGTC
NM_006373.3       CACACGACTGACTACGTGGATGAGATCAAGAAGATTTCCCCTAAAGGAGTGGACATTGTC
                  ************************************************************

HsCD00004822      ATGGACCCTCTGGGTGGGTCAGATACTGCCAAGGGCTACAACCTCCTGAAACCCATGGGC
NM_006373.3       ATGGACCCTCTGGGTGGGTCAGATACTGCCAAGGGCTACAACCTCCTGAAACCCATGGGC
                  ************************************************************

HsCD00004822      AAAGTCGTCACCTATGGAATGGCCAACCTGCTGACGGGCCCCAAACGGAACCTGATGGCC
NM_006373.3       AAAGTCGTCACCTATGGAATGGCCAACCTGCTGACGGGCCCCAAACGGAACCTGATGGCC
                  ************************************************************

HsCD00004822      CTGGCCCGGACATGGTGGAATCAGTTCAGCGTGACAGCTCTGCAGCTGCTGCAGGCCAAC
NM_006373.3       CTGGCCCGGACATGGTGGAATCAGTTCAGCGTGACAGCTCTGCAGCTGCTGCAGGCCAAC
                  ************************************************************

HsCD00004822      CGGGCTGTGTGTGGCTTCCACCTGGGCTACCTGGATGGTGAGGTGGAGCTGGTCAGTGGT
NM_006373.3       CGGGCTGTGTGTGGCTTCCACCTGGGCTACCTGGATGGTGAGGTGGAGCTGGTCAGTGGT
                  ************************************************************

HsCD00004822      GTGGTGGCCCGCCTCCTGGCTCTGTACAACCAGGGCCACATCAAGCCCCACATTGACTCA
NM_006373.3       GTGGTGGCCCGCCTCCTGGCTCTGTACAACCAGGGCCACATCAAGCCCCACATTGACTCA
                  ************************************************************

HsCD00004822      GTCTGGCCCTTCGAGAAGGTGGCTGATGCCATGAAACAGATGCAGGAGAAGAAGAATGTG
NM_006373.3       GTCTGGCCCTTCGAGAAGGTGGCTGATGCCATGAAACAGATGCAGGAGAAGAAGAATGTG
                  ************************************************************

HsCD00004822      GGCAAGGTCCTCCTGGTTCCAGGGCCAGAGAAGGAGAACTAG
NM_006373.3       GGCAAGGTCCTCCTGGTTCCAGGGCCAGAGAAGGAGAACTAG
                  ******************************************

Pathways

Panther : 5HT1 type receptor mediated signaling pathway
Panther : 5HT2 type receptor mediated signaling pathway
Panther : 5HT3 type receptor mediated signaling pathway
Panther : 5HT4 type receptor mediated signaling pathway
Panther : Adrenaline and noradrenaline biosynthesis
Reactome : Amine compound SLC transporters
Reactome : Na+/Cl- dependent neurotransmitter transporters
Reactome : SLC-mediated transmembrane transport
Reactome : Transmembrane transport of small molecules
Reactome : Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds

Protein

SMART domain : PKS_ER : Enoylreductase
UniProt : E9PDJ5
UniProt : E9PB33
UniProt : Q9BRE4
UniProt : Q96GL6
UniProt : P54219
HPRD : 05220

Clone

Cloning Information : 13450
HIP Master Clone ID : 27649
Original Clone ID : FLH013450.01X

Organism


TAX_ID : 9606
Species Specific ID: 6570

Genome

Chromosome : 17
Map Location : 17q21
Ensembl : ENSG00000108828

Reagents

Labome : VAT1-antibody