DNASU Plasmid Repository • 480.965.5697 | Email

IBSP (Homo sapiens) in pDONR201 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  IBSP
Gene Name:  integrin-binding sialoprotein
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH127871.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00005038

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 954nts         Open reading frame : 1 to 954
Reference Sequence Alignment

ATGAAGACTGCTTTAATTTTGCTCAGCATTTTGGGAATGGCCTGTGCTTTCTCAATGAAA
AATTTGCATCGAAGAGTCAAAATAGAGGATTCTGAAGAAAATGGGGTCTTTAAGTACAGG
CCACGATATTATCTTTACAAGCATGCCTACTTTTATCCTCATTTAAAACGATTTCCAGTT
CAGGGCAGTAGTGACTCATCCGAAGAAAATGGAGATGACAGTTCAGAAGAGGAGGAGGAA
GAAGAGGAGACTTCAAATGAAGGAGAAAACAATGAAGAATCGAATGAAGATGAAGACTCT
GAGGCTGAGAATACCACACTTTCTGCTACAACACTGGGCTATGGAGAGGACGCCACGCCT
GGCACAGGGTATACAGGGTTAGCTGCAATCCAGCTTCCCAAGAAGGCTGGGGATATAACA
AACAAAGCTACAAAAGAGAAGGAAAGTGATGAAGAAGAAGTGGAGGAAGAGGAAGGAAAT
GAAAACGAAGAAAGCGAAGCAGAAGTGGATGAAAACGAACAAGGCATAAACGGCACCAGT
ACCAACAGCACAGAGGCAGAAAACGGCAACGGCAGCAGCGGAGGAGACAATGGAGAAGAA
GGGGAAGAAGAAAGTGTCACTGGAGCCAATGCAGAAGGCACCACAGAGACCGGAGGGCAG
GGCAAGGGCACCTCGAAGACAACAACCTCTCCAAATGGTGGGTTTGAACCTACAACCCCA
CCACAAGTCTATAGAACCACTTCCCCACCTTTTGGGAAAACCACCACCGTTGAATACGAG
GGGGAGTACGAATACACGGGCGTCAATGAATACGACAATGGATATGAAATCTATGAAAGT
GAGAACGGGGAACCTCGTGGGGACAATTACCGAGCCTATGAAGATGAGTACAGCTACTTT
AAAGGACAAGGCTACGATGGCTATGATGGTCAGAATTACTACCACCACCAGGAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1025
Start on reference sequence 72
Mutations:
None
Discrepancy:
a461t,E154V
                                

5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IBSP
Symbol Nomenclature : IBSP
SYNONYM : BNSP; BSP; BSP-II; SP-II
Designation : BSP II|bone sialoprotein 2|bone sialoprotein II|cell-binding sialoprotein
Full Nomenclature : integrin-binding sialoprotein
GENEID : 3381
GI : 60831118
GenBank Accession : AY893921
HGNC : 5341
MIM : 147563
Target GenBank: J05213

 

Reference Sequence Alignment

  

HsCD00005038      ATGAAGACTGCTTTAATTTTGCTCAGCATTTTGGGAATGGCCTGTGCTTTCTCAATGAAA
NM_004967.3       ATGAAGACTGCTTTAATTTTGCTCAGCATTTTGGGAATGGCCTGTGCTTTCTCAATGAAA
                  ************************************************************

HsCD00005038      AATTTGCATCGAAGAGTCAAAATAGAGGATTCTGAAGAAAATGGGGTCTTTAAGTACAGG
NM_004967.3       AATTTGCATCGAAGAGTCAAAATAGAGGATTCTGAAGAAAATGGGGTCTTTAAGTACAGG
                  ************************************************************

HsCD00005038      CCACGATATTATCTTTACAAGCATGCCTACTTTTATCCTCATTTAAAACGATTTCCAGTT
NM_004967.3       CCACGATATTATCTTTACAAGCATGCCTACTTTTATCCTCATTTAAAACGATTTCCAGTT
                  ************************************************************

HsCD00005038      CAGGGCAGTAGTGACTCATCCGAAGAAAATGGAGATGACAGTTCAGAAGAGGAGGAGGAA
NM_004967.3       CAGGGCAGTAGTGACTCATCCGAAGAAAATGGAGATGACAGTTCAGAAGAGGAGGAGGAA
                  ************************************************************

HsCD00005038      GAAGAGGAGACTTCAAATGAAGGAGAAAACAATGAAGAATCGAATGAAGATGAAGACTCT
NM_004967.3       GAAGAGGAGACTTCAAATGAAGGAGAAAACAATGAAGAATCGAATGAAGATGAAGACTCT
                  ************************************************************

HsCD00005038      GAGGCTGAGAATACCACACTTTCTGCTACAACACTGGGCTATGGAGAGGACGCCACGCCT
NM_004967.3       GAGGCTGAGAATACCACACTTTCTGCTACAACACTGGGCTATGGAGAGGACGCCACGCCT
                  ************************************************************

HsCD00005038      GGCACAGGGTATACAGGGTTAGCTGCAATCCAGCTTCCCAAGAAGGCTGGGGATATAACA
NM_004967.3       GGCACAGGGTATACAGGGTTAGCTGCAATCCAGCTTCCCAAGAAGGCTGGGGATATAACA
                  ************************************************************

HsCD00005038      AACAAAGCTACAAAAGAGAAGGAAAGTGATGAAGAAGAAGTGGAGGAAGAGGAAGGAAAT
NM_004967.3       AATAAAGCTACAAAAGAGAAGGAAAGTGATGAAGAAGAAGAGGAGGAAGAGGAAGGAAAT
                  ** *************************************:*******************

HsCD00005038      GAAAACGAAGAAAGCGAAGCAGAAGTGGATGAAAACGAACAAGGCATAAACGGCACCAGT
NM_004967.3       GAAAACGAAGAAAGCGAAGCAGAAGTGGATGAAAACGAACAAGGCATAAACGGCACCAGT
                  ************************************************************

HsCD00005038      ACCAACAGCACAGAGGCAGAAAACGGCAACGGCAGCAGCGGAGGAGACAATGGAGAAGAA
NM_004967.3       ACCAACAGCACAGAGGCAGAAAACGGCAACGGCAGCAGCGGAGGAGACAATGGAGAAGAA
                  ************************************************************

HsCD00005038      GGGGAAGAAGAAAGTGTCACTGGAGCCAATGCAGAAGGCACCACAGAGACCGGAGGGCAG
NM_004967.3       GGGGAAGAAGAAAGTGTCACTGGAGCCAATGCAGAAGACACCACAGAGACCGGAAGGCAG
                  *************************************.****************.*****

HsCD00005038      GGCAAGGGCACCTCGAAGACAACAACCTCTCCAAATGGTGGGTTTGAACCTACAACCCCA
NM_004967.3       GGCAAGGGCACCTCGAAGACAACAACCTCTCCAAATGGTGGGTTTGAACCTACAACCCCA
                  ************************************************************

HsCD00005038      CCACAAGTCTATAGAACCACTTCCCCACCTTTTGGGAAAACCACCACCGTTGAATACGAG
NM_004967.3       CCACAAGTCTATAGAACCACTTCCCCACCTTTTGGGAAAACCACCACCGTTGAATACGAG
                  ************************************************************

HsCD00005038      GGGGAGTACGAATACACGGGCGTCAATGAATACGACAATGGATATGAAATCTATGAAAGT
NM_004967.3       GGGGAGTACGAATACACGGGCGCCAATGAATACGACAATGGATATGAAATCTATGAAAGT
                  ********************** *************************************

HsCD00005038      GAGAACGGGGAACCTCGTGGGGACAATTACCGAGCCTATGAAGATGAGTACAGCTACTTT
NM_004967.3       GAGAACGGGGAACCTCGTGGGGACAATTACCGAGCCTATGAAGATGAGTACAGCTACTTT
                  ************************************************************

HsCD00005038      AAAGGACAAGGCTACGATGGCTATGATGGTCAGAATTACTACCACCACCAGGAC
NM_004967.3       AAAGGACAAGGCTACGATGGCTATGATGGTCAGAATTACTACCACCACCAGTGA
                  *************************************************** ..

Pathways

NCI : Beta3 integrin cell surface interactions
Reactome : ECM proteoglycans
Reactome : Extracellular matrix organization
Reactome : Integrin cell surface interactions
WikiPathway : Focal Adhesion
WikiPathway : Osteoblast Signaling

Protein

UniProt : P21815
HPRD : 00951

Clone

Cloning Information : 127871
HIP Master Clone ID : 104020
Original Clone ID : FLH127871.01L

Organism


TAX_ID : 9606
Species Specific ID: 3381

Genome

Chromosome : 4
Map Location : 4q21.1

Reagents

Labome : IBSP-antibody