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KLHL10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL10
Gene Name:  kelch-like family member 10
Sequence             
Original Clone ID: ccsbBroadEn_09994
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC067753.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00505394

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1825nts         Open reading frame : 1 to 1825
Reference Sequence Alignment

ATGGAGATGGAGAGCGCGGCGGCCTCCACACGTTTCCACCAGCCTCACATGGAGAGGAAG
ATGAGTGCGATGGCCTGTGAGATCTTCAACGAGCTTAGACTAGAGGGCAAGCTCTGCGAC
GTGGTCATCAAGGTCAATGGCTTTGAGTTCAGTGCCCATAAGAACATCCTCTGTAGCTGC
AGTTCCTACTTTAGAGCTTTGTTTACAAGTGGCTGGAACAACACTGAAAAGAAGGTATAC
AACATCCCTGGCATTTCTCCTGACATGATGAAGCTAATCATTGAGTATGCATACACCCGG
ACCGTGCCTATCACACCGGACAATGTGGAGAAACTGCTTGCTGCTGCAGACCAGTTTAAC
ATCATGGGTATCGTCAGGGGTTGCTGCGAGTTCCTCAAGTCAGAGCTGTGCTTGGATAAT
TGTATCGGCATCTGTAAGTTCACGGACTACTACTACTGTCCTGAGCTGAGGCAGAAGGCC
TACATGTTCATACTACACAACTTTGAGGAGATGGTGAAAGTCTCGGCAGAATTTTTAGAG
CTCTCGGTCACTGAACTTAAGGATATCATTGAGAAAGATGAGCTCAATGTCAAACAGGAA
GATGCTGTATTTGAGGCCATTTTAAAGTGGATTTCTCATGACCCCCAAAATAGAAAGCAG
CACATTTCAATTTTGCTTCCTAAGGTTCGCCTGGCCCTAATGCATGCTGAGTACTTCATG
AACAATGTTAAGATGAATGACTATGTCAAAGACAGTGAGGAATGCAAACCAGTCATCATT
AATGCCCTAAAGGCCATGTATGACCTCAACATGAATGGACCCTCTAATTCTGATTTCACC
AACCCACTCACCAGACCACGCTTGCCCTATGCCATCCTCTTTGCAATTGGTGGCTGGAGT
GGTGGGAGCCCCACCAATGCCATTGAGGCATATGACGCTCGGGCAGACAGATGGGTGAAT
GTTACTTGTGAGGAAGAGAGTCCCCGTGCCTACCATGGGGCAGCCTATTTGAAAGGCTAT
GTGTATATCATTGGGGGGTTTGATAGTGTAGACTATTTCAATAGTGTTAAGCGTTTTGAC
CCAGTCAAGAAAACTTGGCATCAGGTGGCCCCGATGCACTCCAGACGTTGCTATGTCAGT
GTGACAGTCCTCGGCAATTTTATTTATGCCATGGGAGGATTTGATGGCTACGTGCGTCTA
AGCACTGCTGAACGTTATGAGCCAGAGACCAATCAATGGACACTCATCGCCCCCATGCAC
GAACAGAGGAGTGATGCAAGCGCCACAACACTTTATGGGAAGGTCTACATATGTGGTGGG
TTTAATGGAAACGAGTGCCTGTTCACAGCAGAAGTGTATAACACTGAAAGTAATCAGTGG
ACAGTCATAGCACCCATGAGAAGCAGGAGGAGTGGAATAGGCGTGATTGCTTATGGAGAA
CATGTATATGCGGTAGGTGGCTTTGATGGAGCTAATCGACTTAGGAGTGCCGAAGCCTAC
AGCCCTGTGGCTAACACTTGGCGCACAATCCCCACTATGTTTAATCCTCGTAGCAATTTT
GGCATCGAGGTGGTGGATGACCTCTTGTTTGTGGTTGGTGGCTTTAATGGTTTTACCACC
ACCTTTAATGTTGAGTGCTATGATGAAAAGACCGATGAGTGGTATGATGCTCATGACATG
AGTATATACCGCAGTGCTCTGAGCTGCTGTGTAGTACCAGGGCTGGCCAATGTTGAGGAA
TATGCAGCTAGACGGGACAACTTCCCAGGATTAGAACTGCGAGATGAAGTAAAATATTCT
GCTTCGACAAGTACCCTACCTGTA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL10
Symbol Nomenclature : KLHL10
SYNONYM : SPGF11
Designation : kelch-like 10|kelch-like protein 10
Full Nomenclature : kelch-like family member 10
GENEID : 317719
GenBank Accession : BC160168
HGNC : 18829
MIM : 608778
Vega : OTTHUMG00000152510
Target GenBank: BC160168

 

Reference Sequence Alignment

  

HsCD00505394        ------------------------------------------------------------
XM_005257273.1      ATGGCCGCGATCCGGCCTCAATTGACTTGGATTTCCTTGAGTTCGGTGTCTAGGGAGCCA
NM_152467.3         ------------------------------------------------------------
                                                                                

HsCD00505394        ------------------------------------------------------------
XM_005257273.1      AGTGTTGGGCTGTGGAAGGTAGATGAATGCTCCAGGGATAGAGACCTGTATCAGGGTGCC
NM_152467.3         ------------------------------------------------------------
                                                                                

HsCD00505394        ATGGAGATGGAGAGCGCGGCGGCCTCCACACGTTTCCACCAGCCTCACATGGAGAGGAAG
XM_005257273.1      ATGGAGATGGAGAGCGCGGCGGCCTCCACACGTTTCCACCAGCCTCACATGGAGAGGAAG
NM_152467.3         ATGGAGATGGAGAGCGCGGCGGCCTCCACACGTTTCCACCAGCCTCACATGGAGAGGAAG
                    ************************************************************

HsCD00505394        ATGAGTGCGATGGCCTGTGAGATCTTCAACGAGCTTAGACTAGAGGGCAAGCTCTGCGAC
XM_005257273.1      ATGAGTGCGATGGCCTGTGAGATCTTCAACGAGCTTAGACTAGAGGGCAAGCTCTGCGAC
NM_152467.3         ATGAGTGCGATGGCCTGTGAGATCTTCAACGAGCTTAGACTAGAGGGCAAGCTCTGCGAC
                    ************************************************************

HsCD00505394        GTGGTCATCAAGGTCAATGGCTTTGAGTTCAGTGCCCATAAGAACATCCTCTGTAGCTGC
XM_005257273.1      GTGGTCATCAAGGTCAATGGCTTTGAGTTCAGTGCCCATAAGAACATCCTCTGTAGCTGC
NM_152467.3         GTGGTCATCAAGGTCAATGGCTTTGAGTTCAGTGCCCATAAGAACATCCTCTGTAGCTGC
                    ************************************************************

HsCD00505394        AGTTCCTACTTTAGAGCTTTGTTTACAAGTGGCTGGAACAACACTGAAAAGAAGGTATAC
XM_005257273.1      AGTTCCTACTTTAGAGCTTTGTTTACAAGTGGCTGGAACAACACTGAAAAGAAGGTATAC
NM_152467.3         AGTTCCTACTTTAGAGCTTTGTTTACAAGTGGCTGGAACAACACTGAAAAGAAGGTATAC
                    ************************************************************

HsCD00505394        AACATCCCTGGCATTTCTCCTGACATGATGAAGCTAATCATTGAGTATGCATACACCCGG
XM_005257273.1      AACATCCCTGGCATTTCTCCCGACATGATGAAGCTAATCATTGAGTATGCATACACCCGG
NM_152467.3         AACATCCCTGGCATTTCTCCCGACATGATGAAGCTAATCATTGAGTATGCATACACCCGG
                    ******************** ***************************************

HsCD00505394        ACCGTGCCTATCACACCGGACAATGTGGAGAAACTGCTTGCTGCTGCAGACCAGTTTAAC
XM_005257273.1      ACCGTGCCTATCACACCGGACAATGTGGAGAAACTGCTTGCTGCTGCAGACCAGTTTAAC
NM_152467.3         ACCGTGCCTATCACACCGGACAATGTGGAGAAACTGCTTGCTGCTGCAGACCAGTTTAAC
                    ************************************************************

HsCD00505394        ATCATGGGTATCGTCAGGGGTTGCTGCGAGTTCCTCAAGTCAGAGCTGTGCTTGGATAAT
XM_005257273.1      ATCATGGGTATCGTCAGGGGTTGCTGCGAGTTCCTCAAGTCAGAGCTGTGCTTGGATAAT
NM_152467.3         ATCATGGGTATCGTCAGGGGTTGCTGCGAGTTCCTCAAGTCAGAGCTGTGCTTGGATAAT
                    ************************************************************

HsCD00505394        TGTATCGGCATCTGTAAGTTCACGGACTACTACTACTGTCCTGAGCTGAGGCAGAAGGCC
XM_005257273.1      TGTATCGGCATCTGTAAGTTCACGGACTACTACTACTGTCCTGAGCTGAGGCAGAAGGCC
NM_152467.3         TGTATCGGCATCTGTAAGTTCACGGACTACTACTACTGTCCTGAGCTGAGGCAGAAGGCC
                    ************************************************************

HsCD00505394        TACATGTTCATACTACACAACTTTGAGGAGATGGTGAAAGTCTCGGCAGAATTTTTAGAG
XM_005257273.1      TACATGTTCATACTGCACAACTTTGAGGAGATGGTGAAAGTCTCGGCAGAATTTTTAGAG
NM_152467.3         TACATGTTCATACTGCACAACTTTGAGGAGATGGTGAAAGTCTCGGCAGAATTTTTAGAG
                    **************.*********************************************

HsCD00505394        CTCTCGGTCACTGAACTTAAGGATATCATTGAGAAAGATGAGCTCAATGTCAAACAGGAA
XM_005257273.1      CTCTCGGTCACTGAACTTAAGGATATCATTGAGAAAGATGAGCTCAATGTCAAACAGGAA
NM_152467.3         CTCTCGGTCACTGAACTTAAGGATATCATTGAGAAAGATGAGCTCAATGTCAAACAGGAA
                    ************************************************************

HsCD00505394        GATGCTGTATTTGAGGCCATTTTAAAGTGGATTTCTCATGACCCCCAAAATAGAAAGCAG
XM_005257273.1      GATGCTGTATTTGAGGCCATTTTAAAGTGGATTTCTCATGACCCCCAAAATAGAAAGCAG
NM_152467.3         GATGCTGTATTTGAGGCCATTTTAAAGTGGATTTCTCATGACCCCCAAAATAGAAAGCAG
                    ************************************************************

HsCD00505394        CACATTTCAATTTTGCTTCCTAAGGTTCGCCTGGCCCTAATGCATGCTGAGTACTTCATG
XM_005257273.1      CACATTTCAATTTTGCTTCCTAAGGTTCGCCTGGCCCTAATGCATGCTGAGTACTTCATG
NM_152467.3         CACATTTCAATTTTGCTTCCTAAGGTTCGCCTGGCCCTAATGCATGCTGAGTACTTCATG
                    ************************************************************

HsCD00505394        AACAATGTTAAGATGAATGACTATGTCAAAGACAGTGAGGAATGCAAACCAGTCATCATT
XM_005257273.1      AACAATGTTAAGATGAATGACTATGTCAAAGACAGTGAGGAATGCAAACCAGTCATCATT
NM_152467.3         AACAATGTTAAGATGAATGACTATGTCAAAGACAGTGAGGAATGCAAACCAGTCATCATT
                    ************************************************************

HsCD00505394        AATGCCCTAAAGGCCATGTATGACCTCAACATGAATGGACCCTCTAATTCTGATTTCACC
XM_005257273.1      AATGCCCTAAAGGCCATGTATGACCTCAACATGAATGGACCCTCTAATTCTGATTTCACC
NM_152467.3         AATGCCCTAAAGGCCATGTATGACCTCAACATGAATGGACCCTCTAATTCTGATTTCACC
                    ************************************************************

HsCD00505394        AACCCACTCACCAGACCACGCTTGCCCTATGCCATCCTCTTTGCAATTGGTGGCTGGAGT
XM_005257273.1      AACCCACTCACCAGACCACGCTTGCCCTATGCCATCCTCTTTGCAATTGGTGGCTGGAGT
NM_152467.3         AACCCACTCACCAGACCACGCTTGCCCTATGCCATCCTCTTTGCAATTGGTGGCTGGAGT
                    ************************************************************

HsCD00505394        GGTGGGAGCCCCACCAATGCCATTGAGGCATATGACGCTCGGGCAGACAGATGGGTGAAT
XM_005257273.1      GGTGGGAGCCCCACCAATGCCATTGAGGCATATGACGCTCGGGCAGACAGATGGGTGAAT
NM_152467.3         GGTGGGAGCCCCACCAATGCCATTGAGGCATATGACGCTCGGGCAGACAGATGGGTGAAT
                    ************************************************************

HsCD00505394        GTTACTTGTGAGGAAGAGAGTCCCCGTGCCTACCATGGGGCAGCCTATTTGAAAGGCTAT
XM_005257273.1      GTTACTTGTGAGGAAGAGAGTCCCCGTGCCTACCATGGGGCAGCCTATTTGAAAGGCTAT
NM_152467.3         GTTACTTGTGAGGAAGAGAGTCCCCGTGCCTACCATGGGGCAGCCTATTTGAAAGGCTAT
                    ************************************************************

HsCD00505394        GTGTATATCATTGGGGGGTTTGATAGTGTAGACTATTTCAATAGTGTTAAGCGTTTTGAC
XM_005257273.1      GTGTATATCATTGGGGGGTTTGATAGTGTAGACTATTTCAATAGTGTTAAGCGTTTTGAC
NM_152467.3         GTGTATATCATTGGGGGGTTTGATAGTGTAGACTATTTCAATAGTGTTAAGCGTTTTGAC
                    ************************************************************

HsCD00505394        CCAGTCAAGAAAACTTGGCATCAGGTGGCCCCGATGCACTCCAGACGTTGCTATGTCAGT
XM_005257273.1      CCAGTCAAGAAAACTTGGCATCAGGTGGCCCCGATGCACTCCAGACGTTGCTATGTCAGT
NM_152467.3         CCAGTCAAGAAAACTTGGCATCAGGTGGCCCCGATGCACTCCAGACGTTGCTATGTCAGT
                    ************************************************************

HsCD00505394        GTGACAGTCCTCGGCAATTTTATTTATGCCATGGGAGGATTTGATGGCTACGTGCGTCTA
XM_005257273.1      GTGACAGTCCTCGGCAATTTTATTTATGCCATGGGAGGATTTGATGGCTACGTGCGTCTA
NM_152467.3         GTGACAGTCCTCGGCAATTTTATTTATGCCATGGGAGGATTTGATGGCTACGTGCGTCTA
                    ************************************************************

HsCD00505394        AGCACTGCTGAACGTTATGAGCCAGAGACCAATCAATGGACACTCATCGCCCCCATGCAC
XM_005257273.1      AACACTGCTGAACGTTATGAGCCAGAGACCAATCAATGGACACTCATCGCCCCCATGCAC
NM_152467.3         AACACTGCTGAACGTTATGAGCCAGAGACCAATCAATGGACACTCATCGCCCCCATGCAC
                    *.**********************************************************

HsCD00505394        GAACAGAGGAGTGATGCAAGCGCCACAACACTTTATGGGAAGGTCTACATATGTGGTGGG
XM_005257273.1      GAACAGAGGAGTGATGCAAGCGCCACAACACTTTATGGGAAGGTCTACATATGTGGTGGG
NM_152467.3         GAACAGAGGAGTGATGCAAGCGCCACAACACTTTATGGGAAGGTCTACATATGTGGTGGG
                    ************************************************************

HsCD00505394        TTTAATGGAAACGAGTGCCTGTTCACAGCAGAAGTGTATAACACTGAAAGTAATCAGTGG
XM_005257273.1      TTTAATGGAAACGAGTGCCTGTTCACAGCAGAAGTGTATAACACTGAAAGTAATCAGTGG
NM_152467.3         TTTAATGGAAACGAGTGCCTGTTCACAGCAGAAGTGTATAACACTGAAAGTAATCAGTGG
                    ************************************************************

HsCD00505394        ACAGTCATAGCACCCATGAGAAGCAGGAGGAGTGGAATAGGCGTGATTGCTTATGGAGAA
XM_005257273.1      ACAGTCATAGCACCCATGAGAAGCAGGAGGAGTGGAATAGGCGTGATTGCTTATGGAGAA
NM_152467.3         ACAGTCATAGCACCCATGAGAAGCAGGAGGAGTGGAATAGGCGTGATTGCTTATGGAGAA
                    ************************************************************

HsCD00505394        CATGTATATGCGGTAGGTGGCTTTGATGGAGCTAATCGACTTAGGAGTGCCGAAGCCTAC
XM_005257273.1      CATGTATATGCGGTAGGTGGCTTTGATGGAGCTAATCGACTTAGGAGTGCCGAAGCCTAC
NM_152467.3         CATGTATATGCGGTAGGTGGCTTTGATGGAGCTAATCGACTTAGGAGTGCCGAAGCCTAC
                    ************************************************************

HsCD00505394        AGCCCTGTGGCTAACACTTGGCGCACAATCCCCACTATGTTTAATCCTCGTAGCAATTTT
XM_005257273.1      AGCCCTGTGGCTAACACTTGGCGCACAATCCCCACTATGTTTAATCCTCGTAGCAATTTT
NM_152467.3         AGCCCTGTGGCTAACACTTGGCGCACAATCCCCACTATGTTTAATCCTCGTAGCAATTTT
                    ************************************************************

HsCD00505394        GGCATCGAGGTGGTGGATGACCTCTTGTTTGTGGTTGGTGGCTTTAATGGTTTTACCACC
XM_005257273.1      GGCATCGAGGTGGTGGATGACCTCTTGTTTGTGGTGGGTGGCTTTAATGGTTTTACCACC
NM_152467.3         GGCATCGAGGTGGTGGATGACCTCTTGTTTGTGGTGGGTGGCTTTAATGGTTTTACCACC
                    *********************************** ************************

HsCD00505394        ACCTTTAATGTTGAGTGCTATGATGAAAAGACCGATGAGTGGTATGATGCTCATGACATG
XM_005257273.1      ACCTTTAATGTTGAGTGCTATGATGAAAAGACCGATGAGTGGTATGATGCTCATGACATG
NM_152467.3         ACCTTTAATGTTGAGTGCTATGATGAAAAGACCGATGAGTGGTATGATGCTCATGACATG
                    ************************************************************

HsCD00505394        AGTATATACCGCAGTGCTCTGAGCTGCTGTGTAGTACCAGGGCTGGCCAATGTTGAGGAA
XM_005257273.1      AGTATATACCGCAGTGCTCTGAGCTGCTGTGTAGTACCAGGGCTGGCCAATGTTGAGGAA
NM_152467.3         AGTATATACCGCAGTGCTCTGAGCTGCTGTGTAGTACCAGGGCTGGCCAATGTTGAGGAA
                    ************************************************************

HsCD00505394        TATGCAGCTAGACGGGACAACTTCCCAGGATTAGAACTGCGAGATGAAGTAAAATATTCT
XM_005257273.1      TATGCAGCTAGACGGGACAACTTCCCAGGATTAGCACTGCGAGATGAAGTAAAATATTCT
NM_152467.3         TATGCAGCTAGACGGGACAACTTCCCAGGATTAGCACTGCGAGATGAAGTAAAATATTCT
                    **********************************.*************************

HsCD00505394        GCTTCGACAAGTACCCTACCTGTA---
XM_005257273.1      GCTTCGACAAGTACCCTACCTGTATGA
NM_152467.3         GCTTCGACAAGTACCCTACCTGTATGA
                    ************************   

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
HPRD : 12296

Clone

Cloning Information : 665580
Original Clone ID : ccsbBroadEn_09994

Organism


TAX_ID : 9606
Species Specific ID: 317719

Genome

Chromosome : 17
Map Location : 17q21.2
Ensembl : ENSG00000161594

Reagents

Labome : KLHL10-antibody