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KLHL1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL1
Gene Name:  kelch-like family member 1
Sequence             
Original Clone ID: ccsbBroadEn_08750
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC022460.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00505592

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2245nts         Open reading frame : 1 to 2245
Reference Sequence Alignment

ATGTCAGGCTCTGGGCGAAAAGACTTCGATGTGAAGCACATTCTGCGACTCCGCTGGAAA
CTCTTCAGCCACCCGTCTCCTTCCACCGGCGGCCCGGCGGGGGGAGGCTGCCTGCAACAG
GACGGCAGTGGCAGCTTTGAGCACTGGGGACCCAGCCAGAGTCGCCTGCTCAAAAGCCAA
GAGAGAAGCGGTGTGAGCACTTTCTGGAAGAAGCCTTCCTCCTCTTCCTCTTCATCGTCC
TCCCCGTCCTCTTCCTCCTCTTCCTTCAATCCGCTGAATGGCACCCTGCTTCCAGTTGCC
ACGAGGCTGCAGCAAGGGGCTCCTGGGCAGGGCACTCAGCAGCCAGCCAGGACTCTCTTC
TACGTGGAGTCACTAGAGGAGGAGGTGGTGCCAGGCATGGACTTTCCTGGACCACACGAA
AAAGGGCTGGTTCTGCAAGAGCTCAAAGTGGAGCCAGACAACTCTAGCCAGGCAACAGGT
GAAGGATGTGGACACAGGCTGTCATCAACCGGCCATTCAATGACACCTCAAAGTGATTTG
GACTCCAGTAGCTCTGAAGAATTCTATCAAGCTGTTCATCATGCTGAGCAAACCTTCAGA
AAGATGGAAAGTTATTTGAAGCAGCAGCAACTTTGTGATGTTATCCTGATTGTTGGGAAC
CGAAAGATACCTGCACATAGGCTTGTTCTGAGTTCAGTCTCCGACTATTTTGCGGCCATG
TTTACAAGTGATGTTTGTGAAGCCAAGCAAGAGGAGATCAAAATGGAAGGCATAGACCCC
AATGCTCTCTGGGACCTTGTCCAATTTGCATATACAGGCTGCTTGGAATTAAAAGAGGAC
ACCATTGAGAACCTTCTTGCTGCAGCGTGCCTTCTTCAGCTTCCACAGGTGGTGGAAGTG
TGCTGCCACTTCCTCATGAAGCTTTTGCATCCATCTAACTGTTTAGGAATTCGAGCCTTC
GCAGATGCTCAAGGATGCATTGAGTTAATGAAGGTGGCCCACAGCTACACAATGGAAAAC
ATAATGGAAGTTATCAGAAATCAAGAGTTTTTACTCCTTCCAGCTGAGGAGCTCCATAAA
CTACTGGCCAGTGATGATGTCAATGTTCCTGATGAAGAAACCATCTTCCATGCATTGATG
ATGTGGGTCAAGTATGACATGCAGAGTAGGTGCAATGACCTGAGCATGCTTCTTGCCTTT
ATAAGACTGCCACTGCTTCCGCCACAGATATTGGCTGACCTAGAAAATCATGCATTATTT
AAGAATGATGTGGAATGTCAAAAGCTGATTCTAGAAGCAATGAAATACCATCTATTGCCA
GAAAGAAGAACTTTAATGCAAAGTCCGAGAACTAAACCCAGAAAATCTACAGTCGGAACT
TTGTATGCTGTAGGAGGAATGGATAACAACAAAGGAGCTACAACTATAGAGAAATATGAT
CTGAGAACAAATCTGTGGATCCAGGCAGGGATGATGAATGGCAGAAGGCTGCAGTTTGGT
GTGGCTGTTATTGATGACAAACTCTTTGTAATTGGAGGTCGAGATGGCTTAAAGACATTG
AACACTGTTGAATGTTACAATCCCAAAACCAAGACATGGACTGTCTTACCACCAATGTCA
ACACACAGACATGGTCTAGGTGTAACAGTACTTGAAGGCCCTATTTATGCTGTGGGAGGC
CATGATGGCTGGAGCTATCTGAATACAGTGGAAAGGTGGGATCCACAGAGTCAACAATGG
ACATTTGTAGCCAGTATGTCAATTGCTCGGAGCACAGTTGGTGTAGCAGCATTGAATGGC
AAGTTGTATTCAGTTGGAGGTCGTGATGGAAGTTCCTGTTTGAGTTCAATGGAATATTAT
GATCCTCATACAAATAAGTGGAACATGTGTGCTCCCATGTGTAAGAGGAGAGGGGGTGTC
GGAGTGGCCACATGTGACGGTTTTCTTTATGCAGTAGGAGGTCATGATGCTCCTGCTTCA
AATCACTGTTCCCGGCTACTGGATTATGTAGAAAGATATGATCCCAAAACAGACACTTGG
ACCATGGTGGCTCCTTTGAGTATGCCCAGAGATGCTGTTGGGGTCTGTCTCCTTGGTGAC
AGATTATATGCTGTTGGTGGCTATGATGGACAGACATACCTCAACACTATGGAATCCTAT
GACCCACAAACTAATGAGTGGACACAGATGGCTTCCTTGAATATTGGGAGAGCAGGTGCC
TGTGTGGTAGTCATCAAGCAACCT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL1
Symbol Nomenclature : KLHL1
SYNONYM : MRP2
Designation : Mayven-related protein 2|kelch-like protein 1
Full Nomenclature : kelch-like family member 1
GENEID : 57626
Locus Tag : RP11-394C23.1
GenBank Accession : DQ894052
HGNC : 6352
MIM : 605332
Vega : OTTHUMG00000017056
Target GenBank: DQ894052

 

Reference Sequence Alignment

  

HsCD00505592      ATGTCAGGCTCTGGGCGAAAAGACTTCGATGTGAAGCACATTCTGCGACTCCGCTGGAAA
NM_020866.2       ATGTCAGGCTCTGGGCGAAAAGACTTCGATGTGAAGCACATTCTGCGACTCCGCTGGAAA
                  ************************************************************

HsCD00505592      CTCTTCAGCCACCCGTCTCCTTCCACCGGCGGCCCGGCGGGGGGAGGCTGCCTGCAACAG
NM_020866.2       CTCTTCAGCCACCCGTCTCCTTCCACCGGCGGCCCGGCGGGGGGAGGCTGCCTGCAACAG
                  ************************************************************

HsCD00505592      GACGGCAGTGGCAGCTTTGAGCACTGGGGACCCAGCCAGAGTCGCCTGCTCAAAAGCCAA
NM_020866.2       GACGGCAGTGGCAGCTTTGAGCACTGGGGACCCAGCCAGAGTCGCCTGCTCAAAAGCCAA
                  ************************************************************

HsCD00505592      GAGAGAAGCGGTGTGAGCACTTTCTGGAAGAAGCCTTCCTCCTCTTCCTCTTCATCGTCC
NM_020866.2       GAGAGAAGCGGTGTGAGCACTTTCTGGAAGAAGCCTTCCTCCTCTTCCTCTTCATCGTCC
                  ************************************************************

HsCD00505592      TCCCCGTCCTCTTCCTCCTCTTCCTTCAATCCGCTGAATGGCACCCTGCTTCCAGTTGCC
NM_020866.2       TCCCCGTCCTCTTCCTCCTCTTCCTTCAATCCGCTGAATGGCACCCTGCTTCCAGTTGCC
                  ************************************************************

HsCD00505592      ACGAGGCTGCAGCAAGGGGCTCCTGGGCAGGGCACTCAGCAGCCAGCCAGGACTCTCTTC
NM_020866.2       ACGAGGCTGCAGCAAGGGGCTCCTGGGCAGGGCACTCAGCAGCCAGCCAGGACTCTCTTC
                  ************************************************************

HsCD00505592      TACGTGGAGTCACTAGAGGAGGAGGTGGTGCCAGGCATGGACTTTCCTGGACCACACGAA
NM_020866.2       TACGTGGAGTCACTAGAGGAGGAGGTGGTGCCAGGCATGGACTTTCCTGGACCACACGAA
                  ************************************************************

HsCD00505592      AAAGGGCTGGTTCTGCAAGAGCTCAAAGTGGAGCCAGACAACTCTAGCCAGGCAACAGGT
NM_020866.2       AAAGGGCTGGTTCTGCAAGAGCTCAAAGTGGAGCCAGACAACTCTAGCCAGGCAACAGGT
                  ************************************************************

HsCD00505592      GAAGGATGTGGACACAGGCTGTCATCAACCGGCCATTCAATGACACCTCAAAGTGATTTG
NM_020866.2       GAAGGATGTGGACACAGGCTGTCATCAACCGGCCATTCAATGACACCTCAAAGTGATTTG
                  ************************************************************

HsCD00505592      GACTCCAGTAGCTCTGAAGAATTCTATCAAGCTGTTCATCATGCTGAGCAAACCTTCAGA
NM_020866.2       GACTCCAGTAGCTCTGAAGAATTCTATCAAGCTGTTCATCATGCTGAGCAAACCTTCAGA
                  ************************************************************

HsCD00505592      AAGATGGAAAGTTATTTGAAGCAGCAGCAACTTTGTGATGTTATCCTGATTGTTGGGAAC
NM_020866.2       AAGATGGAAAGTTATTTGAAGCAGCAGCAACTTTGTGATGTTATCCTGATTGTTGGGAAC
                  ************************************************************

HsCD00505592      CGAAAGATACCTGCACATAGGCTTGTTCTGAGTTCAGTCTCCGACTATTTTGCGGCCATG
NM_020866.2       CGAAAGATACCTGCACATAGGCTTGTTCTGAGTTCAGTCTCCGACTATTTTGCGGCCATG
                  ************************************************************

HsCD00505592      TTTACAAGTGATGTTTGTGAAGCCAAGCAAGAGGAGATCAAAATGGAAGGCATAGACCCC
NM_020866.2       TTTACAAGTGATGTTTGTGAAGCCAAGCAAGAGGAGATCAAAATGGAAGGCATAGACCCC
                  ************************************************************

HsCD00505592      AATGCTCTCTGGGACCTTGTCCAATTTGCATATACAGGCTGCTTGGAATTAAAAGAGGAC
NM_020866.2       AATGCTCTCTGGGACCTTGTCCAATTTGCATATACAGGCTGCTTGGAATTAAAAGAGGAC
                  ************************************************************

HsCD00505592      ACCATTGAGAACCTTCTTGCTGCAGCGTGCCTTCTTCAGCTTCCACAGGTGGTGGAAGTG
NM_020866.2       ACCATTGAGAACCTTCTTGCTGCAGCGTGCCTTCTTCAGCTTCCACAGGTGGTGGAAGTG
                  ************************************************************

HsCD00505592      TGCTGCCACTTCCTCATGAAGCTTTTGCATCCATCTAACTGTTTAGGAATTCGAGCCTTC
NM_020866.2       TGCTGCCACTTCCTCATGAAGCTTTTGCATCCATCTAACTGTTTAGGAATTCGAGCCTTC
                  ************************************************************

HsCD00505592      GCAGATGCTCAAGGATGCATTGAGTTAATGAAGGTGGCCCACAGCTACACAATGGAAAAC
NM_020866.2       GCAGATGCTCAAGGATGCATTGAGTTAATGAAGGTGGCCCACAGCTACACAATGGAAAAC
                  ************************************************************

HsCD00505592      ATAATGGAAGTTATCAGAAATCAAGAGTTTTTACTCCTTCCAGCTGAGGAGCTCCATAAA
NM_020866.2       ATAATGGAAGTTATCAGAAATCAAGAGTTTTTACTCCTTCCAGCTGAGGAGCTCCATAAA
                  ************************************************************

HsCD00505592      CTACTGGCCAGTGATGATGTCAATGTTCCTGATGAAGAAACCATCTTCCATGCATTGATG
NM_020866.2       CTACTGGCCAGTGATGATGTCAATGTTCCTGATGAAGAAACCATCTTCCATGCATTGATG
                  ************************************************************

HsCD00505592      ATGTGGGTCAAGTATGACATGCAGAGTAGGTGCAATGACCTGAGCATGCTTCTTGCCTTT
NM_020866.2       ATGTGGGTCAAGTATGACATGCAGAGTAGATGCAATGACCTGAGCATGCTTCTTGCCTTT
                  *****************************.******************************

HsCD00505592      ATAAGACTGCCACTGCTTCCGCCACAGATATTGGCTGACCTAGAAAATCATGCATTATTT
NM_020866.2       ATAAGACTGCCACTGCTTCCACCACAGATATTGGCTGACCTAGAAAATCATGCATTATTT
                  ********************.***************************************

HsCD00505592      AAGAATGATGTGGAATGTCAAAAGCTGATTCTAGAAGCAATGAAATACCATCTATTGCCA
NM_020866.2       AAGAATGATCTGGAATGTCAAAAGCTGATTCTAGAAGCAATGAAATACCATCTATTGCCA
                  ********* **************************************************

HsCD00505592      GAAAGAAGAACTTTAATGCAAAGTCCGAGAACTAAACCCAGAAAATCTACAGTCGGAACT
NM_020866.2       GAAAGAAGAACTTTAATGCAAAGTCCGAGAACTAAACCCAGAAAATCTACAGTCGGAACT
                  ************************************************************

HsCD00505592      TTGTATGCTGTAGGAGGAATGGATAACAACAAAGGAGCTACAACTATAGAGAAATATGAT
NM_020866.2       TTGTATGCTGTAGGAGGAATGGATAACAACAAAGGAGCTACAACTATAGAGAAATATGAT
                  ************************************************************

HsCD00505592      CTGAGAACAAATCTGTGGATCCAGGCAGGGATGATGAATGGCAGAAGGCTGCAGTTTGGT
NM_020866.2       CTGAGAACAAATCTGTGGATCCAGGCAGGGATGATGAATGGCAGAAGGCTGCAGTTTGGT
                  ************************************************************

HsCD00505592      GTGGCTGTTATTGATGACAAACTCTTTGTAATTGGAGGTCGAGATGGCTTAAAGACATTG
NM_020866.2       GTGGCTGTTATTGATGACAAACTCTTTGTAATTGGAGGTCGAGATGGCTTAAAGACATTG
                  ************************************************************

HsCD00505592      AACACTGTTGAATGTTACAATCCCAAAACCAAGACATGGACTGTCTTACCACCAATGTCA
NM_020866.2       AACACTGTTGAATGTTACAATCCCAAAACCAAGACATGGACTGTCTTACCACCAATGTCA
                  ************************************************************

HsCD00505592      ACACACAGACATGGTCTAGGTGTAACAGTACTTGAAGGCCCTATTTATGCTGTGGGAGGC
NM_020866.2       ACACACAGACATGGTCTAGGTGTAACAGTACTTGAAGGCCCTATTTATGCTGTGGGAGGC
                  ************************************************************

HsCD00505592      CATGATGGCTGGAGCTATCTGAATACAGTGGAAAGGTGGGATCCACAGAGTCAACAATGG
NM_020866.2       CATGATGGCTGGAGCTATCTGAATACAGTGGAAAGGTGGGATCCACAGAGTCAACAATGG
                  ************************************************************

HsCD00505592      ACATTTGTAGCCAGTATGTCAATTGCTCGGAGCACAGTTGGTGTAGCAGCATTGAATGGC
NM_020866.2       ACATTTGTAGCCAGTATGTCAATTGCTCGGAGCACAGTTGGTGTAGCAGCATTGAATGGC
                  ************************************************************

HsCD00505592      AAGTTGTATTCAGTTGGAGGTCGTGATGGAAGTTCCTGTTTGAGTTCAATGGAATATTAT
NM_020866.2       AAGTTGTATTCAGTTGGAGGTCGTGATGGAAGTTCCTGTTTGAGTTCAATGGAATATTAT
                  ************************************************************

HsCD00505592      GATCCTCATACAAATAAGTGGAACATGTGTGCTCCCATGTGTAAGAGGAGAGGGGGTGTC
NM_020866.2       GATCCTCATACAAATAAGTGGAACATGTGTGCTCCCATGTGTAAGAGGAGAGGGGGTGTC
                  ************************************************************

HsCD00505592      GGAGTGGCCACATGTGACGGTTTTCTTTATGCAGTAGGAGGTCATGATGCTCCTGCTTCA
NM_020866.2       GGAGTGGCCACATGTGACGGTTTTCTTTATGCAGTAGGAGGTCATGATGCTCCTGCTTCA
                  ************************************************************

HsCD00505592      AATCACTGTTCCCGGCTACTGGATTATGTAGAAAGATATGATCCCAAAACAGACACTTGG
NM_020866.2       AATCACTGTTCCCGGCTACTGGATTATGTAGAAAGATATGATCCCAAAACAGACACTTGG
                  ************************************************************

HsCD00505592      ACCATGGTGGCTCCTTTGAGTATGCCCAGAGATGCTGTTGGGGTCTGTCTCCTTGGTGAC
NM_020866.2       ACCATGGTGGCTCCTTTGAGTATGCCCAGAGATGCTGTTGGGGTCTGTCTCCTTGGTGAC
                  ************************************************************

HsCD00505592      AGATTATATGCTGTTGGTGGCTATGATGGACAGACATACCTCAACACTATGGAATCCTAT
NM_020866.2       AGATTATATGCTGTTGGTGGCTATGATGGACAGACATACCTCAACACTATGGAATCCTAT
                  ************************************************************

HsCD00505592      GACCCACAAACTAATGAGTGGACACAGATGGCTTCCTTGAATATTGGGAGAGCAGGTGCC
NM_020866.2       GACCCACAAACTAATGAGTGGACACAGATGGCTTCCTTGAATATTGGGAGAGCAGGTGCC
                  ************************************************************

HsCD00505592      TGTGTGGTAGTCATCAAGCAACCT---
NM_020866.2       TGTGTGGTAGTCATCAAGCAACCTTGA
                  ************************   

Protein

SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
HPRD : 05623

Clone

Cloning Information : 614567
Original Clone ID : ccsbBroadEn_08750

Organism


TAX_ID : 9606
Species Specific ID: 57626

Genome

Chromosome : 13
Map Location : 13q21
Ensembl : ENSG00000150361

Reagents

Labome : KLHL1-antibody