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LGALS14 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS14
Gene Name:  lectin, galactoside-binding, soluble, 14
Sequence             
Original Clone ID: ccsbBroadEn_08653
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC022257.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00506204

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 418nts         Open reading frame : 1 to 418
Reference Sequence Alignment

ATGTCATCACTACCCGTACCATACACACTGCCTGTTTCCTTGCCTGTTGGTTCGTGCGTG
ATAATCACAGGGACACCGATCCTCACTTTTGTCAAGGACCCACAGCTGGAGGTGAATTTC
TACACTGGGATGGATGAGGACTCAGATATTGCTTTCCAATTCCGACTGCACTTTGGTCAT
CCTGCAATCATGAACAGTCGTGTGTTTGGCATATGGAGATATGAGGAGAAATGCTACTAT
TTACCCTTTGAAGATGGCAAACCATTTGAGCTGTGCATCTATGTGCGTCACAAGGAATAC
AAGGTAATGGTAAATGGCCAACGCATTTACAACTTTGCCCATCGATTCCCGCCAGCATCT
GTGAAGATGCTGCAAGTCTTGAGAGATATCTCCCTGACCAGAGTGCTTATCAGCGAT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS14
Symbol Nomenclature : LGALS14
SYNONYM : CLC2; PPL13
Designation : Charcot-Leyden crystal protein 2|gal-14|galectin-14|placental protein 13-like|placental protein 13-like protein
Full Nomenclature : lectin, galactoside-binding, soluble, 14
GENEID : 56891
GenBank Accession : HQ447718
HGNC : 30054
MIM : 607260
Vega : OTTHUMG00000183143
Target GenBank: HQ447718

 

Reference Sequence Alignment

  

HsCD00506204      ------------------------------------------------------------
NM_020129.2       ------------------------------------------------------------
NM_203471.1       ATGTCCCTGACCCACAGGCTTCATTTGTGCAAGTACTGGGGCTGTGCTGTCAGTAATGTG
                                                                              

HsCD00506204      ---------------------------ATGTCATCACTACCCGTACCATACACACTGCCT
NM_020129.2       ---------------------------ATGTCATCACTACCCGTACCATACACACTGCCT
NM_203471.1       TGCCGCTTCTGGGAAGGACGTCCATTGCCCTTGATGATTGTGGTACCATACACACTGCCT
                                             .  * .: ..*:   ******************

HsCD00506204      GTTTCCTTGCCTGTTGGTTCGTGCGTGATAATCACAGGGACACCGATCCTCACTTTTGTC
NM_020129.2       GTTTCCTTGCCTGTTGGTTCGTGCGTGATAATCACAGGGACACCGATCCTCACTTTTGTC
NM_203471.1       GTTTCCTTGCCTGTTGGTTCGTGCGTGATAATCACAGGGACACCGATCCTCACTTTTGTC
                  ************************************************************

HsCD00506204      AAGGACCCACAGCTGGAGGTGAATTTCTACACTGGGATGGATGAGGACTCAGATATTGCT
NM_020129.2       AAGGACCCACAGCTGGAGGTGAATTTCTACACTGGGATGGATGAGGACTCAGATATTGCT
NM_203471.1       AAGGACCCACAGCTGGAGGTGAATTTCTACACTGGGATGGATGAGGACTCAGATATTGCT
                  ************************************************************

HsCD00506204      TTCCAATTCCGACTGCACTTTGGTCATCCTGCAATCATGAACAGTCGTGTGTTTGGCATA
NM_020129.2       TTCCAATTCCGACTGCACTTTGGTCATCCTGCAATCATGAACAGTTGTGTGTTTGGCATA
NM_203471.1       TTCCAATTCCGACTGCACTTTGGTCATCCTGCAATCATGAACAGTTGTGTGTTTGGCATA
                  ********************************************* **************

HsCD00506204      TGGAGATATGAGGAGAAATGCTACTATTTACCCTTTGAAGATGGCAAACCATTTGAGCTG
NM_020129.2       TGGAGATATGAGGAGAAATGCTACTATTTACCCTTTGAAGATGGCAAACCATTTGAGCTG
NM_203471.1       TGGAGATATGAGGAGAAATGCTACTATTTACCCTTTGAAGATGGCAAACCATTTGAGCTG
                  ************************************************************

HsCD00506204      TGCATCTATGTGCGTCACAAGGAATACAAGGTAATGGTAAATGGCCAACGCATTTACAAC
NM_020129.2       TGCATCTATGTGCGTCACAAGGAATACAAGGTAATGGTAAATGGCCAACGCATTTACAAC
NM_203471.1       TGCATCTATGTGCGTCACAAGGAATACAAGGTAATGGTAAATGGCCAACGCATTTACAAC
                  ************************************************************

HsCD00506204      TTTGCCCATCGATTCCCGCCAGCATCTGTGAAGATGCTGCAAGTCTTGAGAGATATCTCC
NM_020129.2       TTTGCCCATCGATTCCCGCCAGCATCTGTGAAGATGCTGCAAGTCTTCAGAGATATCTCC
NM_203471.1       TTTGCCCATCGATTCCCGCCAGCATCTGTGAAGATGCTGCAAGTCTTCAGAGATATCTCC
                  *********************************************** ************

HsCD00506204      CTGACCAGAGTGCTTATCAGCGAT---
NM_020129.2       CTGACCAGAGTGCTTATCAGCGATTGA
NM_203471.1       CTGACCAGAGTGCTTATCAGCGATTGA
                  ************************   

Protein

SMART domain : Gal-bind_lectin : Galactoside-binding lectin
SMART domain : GLECT : Galectin
HPRD : 06268

Clone

Cloning Information : 615087
Original Clone ID : ccsbBroadEn_08653

Organism


TAX_ID : 9606
Species Specific ID: 56891

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000006659

Reagents

Labome : LGALS14-antibody