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UBE2B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UBE2B
Gene Name:  ubiquitin-conjugating enzyme E2B
Sequence             
Original Clone ID: ccsbBroadEn_01734
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC005979.1,BC008404.1,BC008470.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00506329

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 457nts         Open reading frame : 1 to 457
Reference Sequence Alignment

ATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCCA
CCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAGTTATA
TTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTAAACTAGTAATAGAATTTTCT
GAAGAATATCCAAATAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAAT
GTGTATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATAT
GATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAGT
CCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATATGAGAAAAGA
GTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2B
Symbol Nomenclature : UBE2B
SYNONYM : E2-17kDa; HHR6B; HR6B; RAD6B; UBC2
Designation : E2 protein|RAD6 homolog B|ubiquitin carrier protein B|ubiquitin-conjugating enzyme E2 B|ubiquitin-conjugating enzyme E2-17 kDa|ubiquitin-conjugating enzyme E2B (RAD6 homolog)|ubiquitin-protein ligase B
Full Nomenclature : ubiquitin-conjugating enzyme E2B
GENEID : 7320
GenBank Accession : DQ896387
HGNC : 12473
MIM : 179095
Vega : OTTHUMG00000129120
Target GenBank: DQ896387

 

Reference Sequence Alignment

  

HsCD00506329      ATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCCA
NM_003336.3       ATGTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCT
NM_003337.2       ATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCCA
                  ***** ******** ***.**.* *********** ******.****.**.***** **:

HsCD00506329      CCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAGTTATA
NM_003336.3       CCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGGTCATT
NM_003337.2       CCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAGTTATA
                  **:*  **:***** ** **:**.** **.********.*** :****** **.** **:

HsCD00506329      TTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTAAACTAGTAATAGAATTTTCT
NM_003336.3       TTCGGGCCTGAAGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAGAATTCACT
NM_003337.2       TTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTAAACTAGTAATAGAATTTTCT
                  ** **.**:********.** *****.*****:**:********:. ********* :**

HsCD00506329      GAAGAATATCCAAATAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAAT
NM_003336.3       GAAGAATATCCAAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAAT
NM_003337.2       GAAGAATATCCAAATAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAAT
                  ***********************:**:*****.*** *.** **.***** *********

HsCD00506329      GTGTATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATAT
NM_003336.3       GTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTAT
NM_003337.2       GTGTATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATAT
                  ** *****:******** ****** *.** **.******** **:***********.***

HsCD00506329      GATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAGT
NM_003336.3       GATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGT
NM_003337.2       GATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAGT
                  *****.***** **  *******.**:********* ********** ***** ** ***

HsCD00506329      CCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATATGAGAAAAGA
NM_003336.3       CCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGT
NM_003337.2       CCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATATGAGAAAAGA
                  *****.** ********:**:***** ** *****.********.********.**..*:

HsCD00506329      GTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCA---
NM_003336.3       GTTTCTGCAATAGTAGAACAAAGCTGGCGTGATTGTTGA
NM_003337.2       GTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCATAA
                  ***** **.**:**:************..***** :   

Pathways

Panther : Ubiquitin proteasome pathway
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System
WikiPathway : Proteasome Degradation

Clone

Cloning Information : 667614
Original Clone ID : ccsbBroadEn_01734

Organism


TAX_ID : 9606
Species Specific ID: 7320

Genome

Chromosome : 5
Map Location : 5q31.1
Ensembl : ENSG00000119048

Reagents

Labome : UBE2B-antibody