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SMUG1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SMUG1
Gene Name:  single-strand-selective monofunctional uracil-DNA glycosylase 1
Sequence             
Original Clone ID: ccsbBroadEn_11750
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC000417.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506537

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 532nts        
Open reading frame : 1 to 532
Reference Sequence Alignment

ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCC
CAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCT
GAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTAT
GCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTACTC
TTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCCCTTTGGGGAA
GTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCCTCCCCAAGAG
CATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGGACCAAGACAAAGC
ATGGGACATGAAATTAAGAGTGAACTTCTTATGGGAGGCTGCAGCTGGATCAGAGGAAAA
ATCCAGTGTGACAGAGTGCAAGTCAGAAGACCTGGCTTTTCATCCCAGCTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SMUG1
Symbol Nomenclature : SMUG1
SYNONYM : FDG; HMUDG; UNG3
Designation : single-strand selective monofunctional uracil DNA glycosylase
Full Nomenclature : single-strand-selective monofunctional uracil-DNA glycosylase 1
GENEID : 23583
HGNC : 17148
MIM : 607753
Vega : OTTHUMG00000160068
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00506537        ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCC
NM_001243789.1      ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCC
NM_001243790.1      ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCC
NM_001243791.1      ATGCCCCAGGCTTTCCTGCTGGGGTCCATCCATGAGCCTGCAGGTGCCCTCATGGAGCCC
                    ************************************************************

HsCD00506537        CAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCT
NM_001243789.1      CAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCT
NM_001243790.1      CAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCT
NM_001243791.1      CAGCCCTGCCCTGGAAGCTTGGCTGAGAGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCT
                    ************************************************************

HsCD00506537        GAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTAT
NM_001243789.1      GAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTAT
NM_001243790.1      GAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTAT
NM_001243791.1      GAGCTGAGCCAGCTGCAGTTTTCGGAGCCTGTGGGCATCATCTACAATCCCGTGGAGTAT
                    ************************************************************

HsCD00506537        GCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTACTC
NM_001243789.1      GCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTACTC
NM_001243790.1      GCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTACTC
NM_001243791.1      GCATGGGAGCCACATCGCAACTACGTGACTCGCTACTGCCAGGGCCCCAAGGAAGTACTC
                    ************************************************************

HsCD00506537        TTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCCCTTTGGGGAA
NM_001243789.1      TTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCCCTTTGGGGAA
NM_001243790.1      TTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCCCTTTGGGGAA
NM_001243791.1      TTCCTGGGCATGAACCCTGGACCTTTTGGCATGGCCCAGACTGGGGTGCCCTTTGGGGAA
                    ************************************************************

HsCD00506537        GTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCCTCCCCAAGAG
NM_001243789.1      GTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCCTCCCCAAGAG
NM_001243790.1      GTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCCTCCCCAAGAG
NM_001243791.1      GTAAGCATGGTCCGGGACTGGTTGGGCATTGTGGGGCCTGTGCTGACCCCTCCCCAAGAG
                    ************************************************************

HsCD00506537        CATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGGACCAAGACAAAGC
NM_001243789.1      CATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGGACCAAGACAAAGC
NM_001243790.1      CATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGGACCAAGACAAAGC
NM_001243791.1      CATCCTAAACGACCAGTGCTGGGACTGGAGTGCCCACAGTCAGAAGGACCAAGACAAAGC
                    ************************************************************

HsCD00506537        ATGGGACATGAAATTAAGAGTGAACTTCTTATGGGAGGCTGCAGCTGGATCAGAGGAAAA
NM_001243789.1      ATGGGACATGAAATTAAGAGTGAACTTCTTATGGGAGGCTGCAGCTGGATCAGAGGAAAA
NM_001243790.1      ATGGGACATGAAATTAAGAGTGAACTTCTTATGGGAGGCTGCAGCTGGATCAGAGGAAAA
NM_001243791.1      ATGGGACATGAAATTAAGAGTGAACTTCTTATGGGAGGCTGCAGCTGGATCAGAGGAAAA
                    ************************************************************

HsCD00506537        ATCCAGTGTGACAGAGTGCAAGTCAGAAGACCTGGCTTTTCATCCCAGCTT---
NM_001243789.1      ATCCAGTGTGACAGAGTGCAAGTCAGAAGACCTGGCTTTTCATCCCAGCTTTGA
NM_001243790.1      ATCCAGTGTGACAGAGTGCAAGTCAGAAGACCTGGCTTTTCATCCCAGCTTTGA
NM_001243791.1      ATCCAGTGTGACAGAGTGCAAGTCAGAAGACCTGGCTTTTCATCCCAGCTTTGA
                    ***************************************************   

Pathways

Reactome : Base Excision Repair
Reactome : Base-Excision Repair, AP Site Formation
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : Cleavage of the damaged pyrimidine
Reactome : Depyrimidination
Reactome : Displacement of DNA glycosylase by APE1
Reactome : DNA Repair
Reactome : Recognition and association of DNA glycosylase with site containing an affected pyrimidine
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of Abasic Sites (AP sites)
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
WikiPathway : Fluoropyrimidine Activity

Protein

HPRD : 06376

Clone

Cloning Information : 667393
Original Clone ID : ccsbBroadEn_11750

Organism


TAX_ID : 9606
Species Specific ID: 23583

Genome

Chromosome : 12
Map Location : 12q13.13
Ensembl : ENSG00000123415

Reagents

Labome : SMUG1-antibody