DNASU Plasmid Repository • 480.965.5697 | Email

CA6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CA6
Gene Name:  carbonic anhydrase VI
Sequence             
Original Clone ID: ccsbBroadEn_10704
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC034350.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00506546

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 538nts         Open reading frame : 1 to 538

ATGAGGGCCCTGGTGCTTCTGCTGTCCCTGTTCCTGCTGGGTGGCCAGGCCCAGCATGTG
TCTGACTGGACCTACTCAGAAGGGGCACTGGACGAAGCGCACTGGCCACAGCACTACCCC
GCCTGTGGGGGCCAGAGACAGTCGCCTATCAACCTACAGAGGACGAAGGTGCGGTACAAC
CCCTCCTTGAAGGGGCTCAATATGACAGGCTATGAGACCCAGGCAGGGGAGTTCCCCATG
GTCAACAATGGCCACACAGATTGGCAGGCGGAACTCTTCCTCCCCTCCTTGTCCTCTCCT
TGTGGAGAAGGGGCGAAGCACAAAGGCCAAAGGCAGTGGCTTTGTTCACCCTCCCTGGAC
AGCTGGTGGGAAGCAATCATTGATGGAGGATCTCTATGGGCAACGCTTGCTGAGTGCGGG
CTTCGTGCTGAGGGCCCAGAAGAGGGAAGGCATATGGAAGAGAAGAGCCCAGCTTCCCAA
GGGGCTTGCAGCGGCTCCCGCCTCCCAATTTCCAGAGGACCCTGTACCTTCTTCCTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CA6
Symbol Nomenclature : CA6
SYNONYM : CA-VI; GUSTIN
Designation : carbonate dehydratase VI|carbonic anhydrase 6|salivary carbonic anhydrase|secreted carbonic anhydrase
Full Nomenclature : carbonic anhydrase VI
GENEID : 765
Locus Tag : RP3-477M7.7
GenBank Accession : JF432775
HGNC : 1380
MIM : 114780
Vega : OTTHUMG00000001763
Target GenBank: JF432775

 

Pathways

Reactome : Metabolism
Reactome : Reversible hydration of carbon dioxide

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
HPRD : 00264

Clone

Cloning Information : 615333
Original Clone ID : ccsbBroadEn_10704

Organism


TAX_ID : 9606
Species Specific ID: 765

Genome

Chromosome : 1
Map Location : 1p36.2
Ensembl : ENSG00000131686

Reagents

Labome : CA6-antibody