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RNASE11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RNASE11
Gene Name:  ribonuclease, RNase A family, 11 (non-active)
Sequence             
Original Clone ID: ccsbBroadEn_04765
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC025410.1
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00506885

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 598nts         Open reading frame : 1 to 598
Reference Sequence Alignment

ATGGAGACCTTTCCTCTGCTGCTGCTCAGCCTGGGCCTGGTTCTTGCAGAAGCATCAGAA
AGCACAATGAAGATAATTAAAGAAGAATTTACAGACGAAGAGATGCAATATGACATGGCA
AAAAGTGGCCAAGAAAAACAGACCATTGAGATATTAATGAACCCGATCCTGTTAGTTAAA
AATACCAGCCTCAGCATGTCCAAGGATGATATGTCTTCCACATTACTGACATTCAGAAGT
TTACATTATAATGACCCCAAGGGAAACAGTTCGGGTAATGACAAAGAGTGTTGCAATGAC
ATGACAGTCTGGAGAAAAGTTTCAGAAGCAAACGGATCGTGCAAGTGGAGCAATAACTTC
ATCCGCAGCTCCACAGAAGTGATGCGCAGGGTCCACAGGGCCCCCAGCTGCAAGTTTGTA
CAGAATCCTGGCATAAGCTGCTGTGAGAGCCTAGAACTGGAAAATACAGTGTGCCAGTTC
ACTACAGGCAAACAATTCCCCAGGTGCCAATACCATAGTGTTACCTCATTAGAGAAGATA
TTGACAGTGCTGACAGGTCATTCTCTGATGAGCTGGTTAGTTTGTGGCTCTAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNASE11
Symbol Nomenclature : RNASE11
SYNONYM : C14orf6; HEL-S-84p; RAJ1
Designation : RNase 11|epididymis secretory sperm binding protein Li 84p|probable ribonuclease 11|ribonuclease 11|ribonuclease A J1
Full Nomenclature : ribonuclease, RNase A family, 11 (non-active)
GENEID : 122651
Locus Tag : UNQ5832/PRO19669
HGNC : 19269
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00506885      ATGGAGACCTTTCCTCTGCTGCTGCTCAGCCTGGGCCTGGTTCTTGCAGAAGCATCAGAA
NM_145250.3       ATGGAGACCTTTCCTCTGCTGCTGCTCAGCCTGGGCCTGGTTCTTGCAGAAGCATCAGAA
                  ************************************************************

HsCD00506885      AGCACAATGAAGATAATTAAAGAAGAATTTACAGACGAAGAGATGCAATATGACATGGCA
NM_145250.3       AGCACAATGAAGATAATTAAAGAAGAATTTACAGACGAAGAGATGCAATATGACATGGCA
                  ************************************************************

HsCD00506885      AAAAGTGGCCAAGAAAAACAGACCATTGAGATATTAATGAACCCGATCCTGTTAGTTAAA
NM_145250.3       AAAAGTGGCCAAGAAAAACAGACCATTGAGATATTAATGAACCCGATCCTGTTAGTTAAA
                  ************************************************************

HsCD00506885      AATACCAGCCTCAGCATGTCCAAGGATGATATGTCTTCCACATTACTGACATTCAGAAGT
NM_145250.3       AATACCAGCCTCAGCATGTCCAAGGATGATATGTCTTCCACATTACTGACATTCAGAAGT
                  ************************************************************

HsCD00506885      TTACATTATAATGACCCCAAGGGAAACAGTTCGGGTAATGACAAAGAGTGTTGCAATGAC
NM_145250.3       TTACATTATAATGACCCCAAGGGAAACAGTTCGGGTAATGACAAAGAGTGTTGCAATGAC
                  ************************************************************

HsCD00506885      ATGACAGTCTGGAGAAAAGTTTCAGAAGCAAACGGATCGTGCAAGTGGAGCAATAACTTC
NM_145250.3       ATGACAGTCTGGAGAAAAGTTTCAGAAGCAAACGGATCGTGCAAGTGGAGCAATAACTTC
                  ************************************************************

HsCD00506885      ATCCGCAGCTCCACAGAAGTGATGCGCAGGGTCCACAGGGCCCCCAGCTGCAAGTTTGTA
NM_145250.3       ATCCGCAGCTCCACAGAAGTGATGCGCAGGGTCCACAGGGCCCCCAGCTGCAAGTTTGTA
                  ************************************************************

HsCD00506885      CAGAATCCTGGCATAAGCTGCTGTGAGAGCCTAGAACTGGAAAATACAGTGTGCCAGTTC
NM_145250.3       CAGAATCCTGGCATAAGCTGCTGTGAGAGCCTAGAACTGGAAAATACAGTGTGCCAGTTC
                  ************************************************************

HsCD00506885      ACTACAGGCAAACAATTCCCCAGGTGCCAATACCATAGTGTTACCTCATTAGAGAAGATA
NM_145250.3       ACTACAGGCAAACAATTCCCCAGGTGCCAATACCATAGTGTTACCTCATTAGAGAAGATA
                  ************************************************************

HsCD00506885      TTGACAGTGCTGACAGGTCATTCTCTGATGAGCTGGTTAGTTTGTGGCTCTAAGTTG---
NM_145250.3       TTGACAGTGCTGACAGGTCATTCTCTGATGAGCTGGTTAGTTTGTGGCTCTAAGTTGTAA
                  *********************************************************   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 15257

Clone

Cloning Information : 666975
Original Clone ID : ccsbBroadEn_04765

Organism


TAX_ID : 9606
Species Specific ID: 122651

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000173464

Reagents

Labome : RNASE11-antibody