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U2AF1L4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  U2AF1L4
Gene Name:  U2 small nuclear RNA auxiliary factor 1-like 4
Sequence             
Original Clone ID: ccsbBroadEn_09799
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC021186.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00506892

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 607nts         Open reading frame : 1 to 607
Reference Sequence Alignment

ATGGCTGAATATTTAGCTTCGATATTCGGGACTGAGAAGGACAAGGTTAACTGCTCTTTT
TACTTTAAGATCGGGGTCTGCCGGCACGGGGACCGGTGCTCCCGGCTTCACAACAAGCCG
ACATTCAGCCAGGAGGTGTTCACAGAACTGCAGGAGAAGTATGGGGAGATTGAAGAGATG
AATGTGTGCGACAACCTTGGGGACCACGTCGTGGGCAACGTCTATGTCAAGTTCCGGAGG
GAGGAGGATGGAGAGCGGGCCGTGGCTGAACTCAGTAACCGCTGGTTCAACGGGCAGGCT
GTGCACGGGAATGTACCCGAGGTGGCTTCTGCAACTTCATGCATCTGCGGCCCATTTCCC
AGAACCTCCAGAGGCAGCTCTATGGGCGGGGACCCAGGCGCAGGTCACCCCCGAGGTTCC
ATACTGGCCACAATCCCCGAGAGAGGAACCATCGGTGTTCCCCTGATCACTGGCATGGCC
GCTTCTGAGGCCCTGGCCCCCTTACCCTTCACCCCCAACAGGGACAGATGTTCCTGGCAG
GACCTCTCCTCAAAGCCCCCTTCACTCTCCTGCCCCATCCTTCCCAGGCTCCCGGGCTCC
ATAATG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : U2AF1L4
Symbol Nomenclature : U2AF1L4
SYNONYM : U2AF1-RS3; U2AF1L3; U2AF1L3V1; U2AF1RS3; U2af26
Designation : U2 auxiliary factor 26|U2 small nuclear RNA auxiliary factor 1-like 3|U2 small nuclear RNA auxiliary factor 1-like protein 3|U2 small nuclear RNA auxiliary factor 1-like protein 4|U2(RNU2) small nuclear RNA auxiliary factor 1-like 3|U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3|U2AF1-like protein 3|splicing factor U2AF 26 kDa subunit
Full Nomenclature : U2 small nuclear RNA auxiliary factor 1-like 4
GENEID : 199746
GenBank Accession : DQ895636
HGNC : 23020
MIM : 601080
Vega : OTTHUMG00000182066
Target GenBank: DQ895636

 

Reference Sequence Alignment

  

HsCD00506892      ATGGCTGAATATTTAGCTTCGATATTCGGGACTGAGAAGGACAAGGTTAACTGCTCTTTT
NM_144987.3       ATGGCTGAATATTTAGCTTCGATATTCGGGACTGAGAAGGACAAGGTTAACTGCTCTTTT
                  ************************************************************

HsCD00506892      TACTTTAAGATCGGGGTCTGCCGGCACGGGGACCGGTGCTCCCGGCTTCACAACAAGCCG
NM_144987.3       TACTTTAAGATCGGGGTCTGCCGGCACGGGGACCGGTGCTCCCGGCTTCACAACAAGCCG
                  ************************************************************

HsCD00506892      ACATTCAGCCAGGAGGTGTTCACAGAACTGCAGGAGAAGTATGGGGAGATTGAAGAGATG
NM_144987.3       ACATTCAGCCAGGAGGTGTTCACAGAACTGCAGGAGAAGTATGGGGAGATTGAAGAGATG
                  ************************************************************

HsCD00506892      AATGTGTGCGACAACCTTGGGGACCACGTCGTGGGCAACGTCTATGTCAAGTTCCGGAGG
NM_144987.3       AATGTGTGCGACAACCTTGGGGACCACCTCGTGGGCAACGTCTATGTCAAGTTCCGGAGG
                  *************************** ********************************

HsCD00506892      GAGGAGGATGGAGAGCGGGCCGTGGCTGAACTCAGTAACCGCTGGTTCAACGGGCAGGCT
NM_144987.3       GAGGAGGATGGAGAGCGGGCCGTGGCTGAACTCAGTAACCGCTGGTTCAACGGGCAGGCT
                  ************************************************************

HsCD00506892      GTGCACGGGAATGTACCCGAGGTGGCTTCTGCAACTTCATGCATCTGCGGCCCATTTCCC
NM_144987.3       GTGCACGGGAATGTACCCGAGGTGGCTTCTGCAACTTCATGCATCTGCGGCCCATTTCCC
                  ************************************************************

HsCD00506892      AGAACCTCCAGAGGCAGCTCTATGGGCGGGGACCCAGGCGCAGGTCACCCCCGAGGTTCC
NM_144987.3       AGAACCTCCAGAGGCAGCTCTATGGGCGGGGACCCAGGCGCAGGTCACCCCCGAGGTTCC
                  ************************************************************

HsCD00506892      ATACTGGCCACAATCCCCGAGAGAGGAACCATCGGTGTTCCCCTGATCACTGGCATGGCC
NM_144987.3       ATACTGGCCACCATCCCCGAGAGAGGAACCATCGGTGTTCCCCTGATCACTGGCATGGCC
                  ***********.************************************************

HsCD00506892      GCTTCTGAGGCCCTGGCCCCCTTACCCTTCACCCCCAACAGGGACAGATGTTCCTGGCAG
NM_144987.3       GCTTCTGAGGCCCTGGCCCCCTTACCCTTCACCCCCAACAGGGACAGATGTTCCTGGCAG
                  ************************************************************

HsCD00506892      GACCTCTCCTCAAAGCCCCCTTCACTCTCCTGCCCCATCCTTCCCAGGCTCCCGGGCTCC
NM_144987.3       GACCTCTCCTCAAAGCCCCCTTCACTCTCCTGCCCCATCCTTCCCAGGCTCCCGGGCTCC
                  ************************************************************

HsCD00506892      ATAATG---
NM_144987.3       ATAATGTAA
                  ******   

Protein

SMART domain : RRM_1 : RNA recognition motif
SMART domain : ZnF_C3H1 : zinc finger
HPRD : 03050

Clone

Cloning Information : 615679
Original Clone ID : ccsbBroadEn_09799

Organism


TAX_ID : 9606
Species Specific ID: 199746

Genome

Chromosome : 19
Map Location : 19q13.12
Ensembl : ENSG00000161265

Reagents

Labome : U2AF1L4-antibody