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SLC6A11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SLC6A11
Gene Name:  solute carrier family 6 (neurotransmitter transporter), member 11
Sequence             
Original Clone ID: ccsbBroadEn_11136
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC036083.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00506940

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 625nts         Open reading frame : 1 to 625
Reference Sequence Alignment

ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAG
TCCGAGGCGCCGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGC
GTCAAGCGCGACAAGGCGGTCCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTG
CTGAGCGTGGCCGGGGAGATCATTGGGCTGGGCAACGTGTGGCGCTTCCCCTACCTGTGC
TACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGGTGTTTTTTATTTGCTGTGGA
ATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGGTGGCATTACG
TGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAAC
TGCTTCACTACTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGT
GTGGAGTTCCAGAAACTGAATGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACC
TCCCCTGTCATGGAGTTTTGGGAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SLC6A11
Symbol Nomenclature : SLC6A11
SYNONYM : GAT-3; GAT3; GAT4
Designation : GABA transporter 3|sodium- and chloride-dependent GABA transporter 3|solute carrier family 6 (neurotransmitter transporter, GABA), member 11|solute carrier family 6 member 11
Full Nomenclature : solute carrier family 6 (neurotransmitter transporter), member 11
GENEID : 6538
GenBank Accession : BC152735
HGNC : 11044
MIM : 607952
Target GenBank: BC152735

 

Reference Sequence Alignment

  

HsCD00506940      ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAG
NM_014229.1       ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAG
                  ************************************************************

HsCD00506940      TCCGAGGCGCCGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGC
NM_014229.1       TCCGAGGCGCCGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGC
                  ************************************************************

HsCD00506940      GTCAAGCGCGACAAGGCGGTCCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTG
NM_014229.1       GTCAAGCGCGACAAGGCGGTCCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTG
                  ************************************************************

HsCD00506940      CTGAGCGTGGCCGGGGAGATCATTGGGCTGGGCAACGTGTGGCGCTTCCCCTACCTGTGC
NM_014229.1       CTGAGCGTGGCCGGGGAGATCATTGGGCTGGGCAACGTGTGGCGCTTCCCCTACCTGTGC
                  ************************************************************

HsCD00506940      TACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGGTGTTTTTTATTTGCTGTGGA
NM_014229.1       TACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGGTGTTTTTTATTTGCTGTGGA
                  ************************************************************

HsCD00506940      ATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGGTGGCATTACG
NM_014229.1       ATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGGTGGCATTACG
                  ************************************************************

HsCD00506940      TGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
NM_014229.1       TGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
                  ************************************************************

HsCD00506940      GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAAC
NM_014229.1       GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAAC
                  ************************************************************

HsCD00506940      TGCTTCACTACTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGT
NM_014229.1       TGCTTCACTACTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGT
                  ************************************************************

HsCD00506940      GTGGAGTTCCAGAAACTGAATGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACC
NM_014229.1       GTGGAGTTCCAGAAACTGAATGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACC
                  ************************************************************

HsCD00506940      TCCCCTGTCATGGAGTTTTGGGAG------------------------------------
NM_014229.1       TCCCCTGTCATGGAGTTTTGGGAGCACCGGGTCCTGGCCATCTCTGACGGGATCGAGCAC
                  ************************                                    

HsCD00506940      ------------------------------------------------------------
NM_014229.1       ATCGGGAACCTTCGCTGGGAGCTGGCCTTGTGTCTCTTGGCAGCCTGGACCATCTGTTAC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       TTCTGTATCTGGAAGGGGACCAAGTCTACAGGAAAGGTTGTATACGTGACTGCGACATTC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       CCCTACATCATGCTGCTGATCCTCCTGATACGAGGGGTCACGTTGCCCGGGGCCTCAGAG
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       GGCATCAAGTTCTACTTGTACCCTGACCTCTCCCGGCTCTCCGACCCCCAGGTCTGGGTA
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       GATGCTGGAACGCAGATCTTTTTCTCCTATGCCATTTGCCTGGGCTGTCTGACCGCTCTG
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       GGAAGTTATAACAATTATAACAACAACTGCTACAGGGACTGCATCATGCTCTGTTGCCTG
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       AACAGCGGCACCAGCTTCGTGGCTGGGTTTGCCATCTTCTCAGTCCTGGGTTTTATGGCG
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       TACGAGCAGGGGGTACCCATTGCTGAGGTGGCAGAGTCAGGCCCCGGCCTGGCCTTTATT
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       GCGTACCCCAAGGCGGTCACCATGATGCCTCTCTCCCCGCTGTGGGCCACCTTGTTCTTC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       ATGATGCTCATCTTCCTGGGCCTGGACAGCCAGTTTGTGTGTGTGGAAAGCCTGGTGACC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       GCCGTGGTGGACATGTACCCCAAGGTTTTCCGGAGGGGTTACCGGCGGGAGCTGCTCATC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       CTAGCCTTGTCTGTTATCTCCTATTTTCTGGGCCTCGTGATGTTAACAGAGGGTGGCATG
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       TACATCTTCCAGCTCTTTGACTCCTATGCCGCCAGTGGGATGTGCCTTCTCTTCGTGGCC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       ATCTTTGAGTGCATCTGCATCGGCTGGGTGTATGGAAGCAACCGGTTCTATGATAACATT
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       GAAGACATGATTGGCTACCGGCCACCGTCGCTCATTAAGTGGTGCTGGATGATCATGACC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       CCTGGGATCTGCGCGGGGATCTTCATCTTCTTCTTGATCAAGTACAAGCCACTCAAGTAC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       AACAACATCTACACCTACCCAGCCTGGGGCTATGGCATTGGCTGGCTCATGGCCCTGTCC
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       TCCATGCTCTGCATCCCGCTCTGGATCTGCATCACAGTGTGGAAGACGGAGGGGACACTG
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       CCCGAGAAACTCCAGAAGTTGACGACCCCCAGCACAGATCTGAAAATGCGGGGCAAGCTT
                                                                              

HsCD00506940      ------------------------------------------------------------
NM_014229.1       GGGGTGAGCCCACGGATGGTGACAGTTAATGACTGTGATGCCAAACTCAAGAGTGACGGG
                                                                              

HsCD00506940      ---------------------------------------
NM_014229.1       ACCATCGCAGCCATCACAGAGAAGGAGACGCACTTCTGA
                                                         

Pathways

Reactome : Amine compound SLC transporters
Reactome : Creatine metabolism
Reactome : GABA synthesis, release, reuptake and degradation
Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Na+/Cl- dependent neurotransmitter transporters
Reactome : Neuronal System
Reactome : Neurotransmitter Release Cycle
Reactome : Reuptake of GABA
Reactome : SLC-mediated transmembrane transport
Reactome : Transmembrane transport of small molecules
Reactome : Transmission across Chemical Synapses
Reactome : Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds

Protein

HPRD : 06406

Clone

Cloning Information : 615821
Original Clone ID : ccsbBroadEn_11136

Organism


TAX_ID : 9606
Species Specific ID: 6538

Genome

Chromosome : 3
Map Location : 3p25.3

Reagents

Labome : GAT-3-antibody