DNASU Plasmid Repository • 480.965.5697 | Email

P2RY11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  PPAN-P2RY11
Gene Name:  PPAN-P2RY11 readthrough
Sequence             
Original Clone ID: ccsbBroadEn_11014
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC009877.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00507047

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 640nts         Open reading frame : 1 to 640
Reference Sequence Alignment

ATGCCCACACTCAGCTTCTCCCACCTGAAGAGGCCGCAGCAGGGGGCGGGCAACTGCAGC
GTGGCCAGGCCCGAGGCCTGCATCAAGTGTCTGGGGACAGCAGACCACGGGCTGGCGGCC
TACAGAGCGTATAGCCTGGTGCTGGCGGGGTTGGGCTGCGGCCTGCCGCTGCTGCTCACG
CTGGCAGCCTACGGCGCCCTCGGGCGGGCCGTGCTACGCAGCCCAGGCATGACTGTGGCC
GAGAAGCTGCGTGTGGCAGCGTTGGTGGCCAGTGGTGTGGCCCTCTACGCCAGCTCCTAT
GTGCCCTACCACATCATGCGGGTGCTCAACGTGGATGCTCGGCGGCGCTGGAGCACCCGC
TGCCCGAGCTTTGCAGACATAGCCCAGGCCACAGCAGCCCTGGAGCTGGGGCCCTACGTG
GGCTACCAGGTGATGCGGGGCCTCATGCCCCTGGCCTTCTGTGTCCACCCTCTACTCTAC
ATGGCCGCAGTGCCCAGCCTGGGCTGCTGCTGCCGACACTGCCCCGGCTACAGGGACAGC
TGGAACCCAGAGGACGCCAAGAGCACTGGCCAAGCCCTGCCCCTCAATGCCACAGCCGCC
CCTAAACCGTCAGAGCCCCAGTCCCGTGAGCTGAGCCAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPAN-P2RY11
Symbol Nomenclature : PPAN-P2RY11
Designation : PPAN-P2RY11 protein|PPAN-P2RY11 readthrough transcript|SSF1/P2Y11 protein
Full Nomenclature : PPAN-P2RY11 readthrough
GENEID : 692312
HGNC : 33526
Vega : OTTHUMG00000150165
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      ATGGGACAGTCAGGGAGGTCCCGGCACCAGAAGCGCGCCCGCGCCCAGGCGCAGCTCCGC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      AACCTCGAGGCCTATGCCGCGAACCCGCACTCGTTCGTGTTCACGCGAGGCTGCACGGGT
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CGCAACATCCGGCAGCTCAGCCTGGACGTGCGGCGGGTCATGGAGCCGCTCACTGCCAGC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CGTCTGCAGGTTCGTAAGAAGAACTCGCTGAAGGACTGCGTGGCAGTGGCTGGGCCCCTC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GGGGTCACACACTTTCTGATCCTGAGCAAAACAGAGACCAATGTCTACTTTAAGCTGATG
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CGCCTCCCAGGAGGCCCCACCTTGACCTTCCAGGTCAAGAAGTACTCGCTGGTGCGTGAT
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GTGGTCTCCTCACTGCGCCGGCACCGCATGCACGAGCAGCAGTTTGCCCACCCACCCCTC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CTGGTACTCAACAGCTTTGGCCCCCATGGTATGCATGTGAAGCTCATGGCCACCATGTTC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CAGAACCTGTTCCCCTCCATCAACGTGCACAAGGTGAACCTGAACACCATCAAGCGCTGC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CTCCTCATCGACTACAACCCCGACTCCCAGGAGCTGGACTTCCGCCACTATAGCATCAAA
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GTTGTTCCTGTGGGCGCGAGTCGCGGGATGAAGAAGCTGCTCCAGGAGAAGTTCCCCAAC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      ATGAGCCGCCTGCAGGACATCAGCGAGCTGCTGGCCACGGGCGCGGGGCTGTCGGAGAGC
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GAGGCAGAGCCTGACGGCGACCACAACATCACAGAGCTGCCTCAGGCTGTCGCTGGCCGT
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GGCAACATGCGGGCCCAGCAGAGTGCAGTGCGGCTCACCGAGATCGGCCCGCGGATGACA
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CTGCAGCTCATCAAGGTCCAGGAGGGCGTCGGGGAGGGCAAAGTGATGTTCCACAGTTTT
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GTGAGCAAGACGGAGGAGGAGCTGCAGGCCATCCTGGAAGCCAAGGAGAAGAAGCTGCGG
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CTGAAGGCGCAGAGGCAGGCCCAGCAGGCCCAGAATGTGCAGCGCAAGCAGGAGCAGCGG
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GAGGCCCACAGAAAGAAGAGCCTGGAGGGCATGAAGAAGGCACGGGTCGGGGGTAGTGAT
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GAAGAGGCCTCTGGGATCCCTTCAAGGACGGCGAGCCTGGAGTTGGGTGAGGACGATGAT
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GAACAGGAAGATGATGACATCGAGTATTTCTGCCAGGCGGTGGGCGAGGCGCCCAGTGAG
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GACCTGTTCCCCGAGGCCAAGCAGAAACGGCTTGCCAAGTCTCCAGGGCGGAAGCGGAAG
NM_002566.4         ------------------------------------------------------------
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CGGTGGGAAATGGATCGAGGTGCCAAGTCCTGCCCTGCCAACTTCTTGGCAGCTGCCGAC
NM_002566.4         ATGGCAGCCAACGTCTCGGGTGCCAAGTCCTGCCCTGCCAACTTCTTGGCAGCTGCCGAC
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GACAAACTCAGTGGGTTCCAGGGGGACTTCCTGTGGCCCATACTGGTGGTTGAGTTCCTG
NM_002566.4         GACAAACTCAGTGGGTTCCAGGGGGACTTCCTGTGGCCCATACTGGTGGTTGAGTTCCTG
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GTGGCCGTGGCCAGCAATGGCCTGGCCCTGTACCGCTTCAGCATCCGGAAGCAGCGCCCA
NM_002566.4         GTGGCCGTGGCCAGCAATGGCCTGGCCCTGTACCGCTTCAGCATCCGGAAGCAGCGCCCA
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      TGGCACCCCGCCGTGGTCTTCTCTGTCCAGCTGGCAGTCAGCGACCTGCTCTGCGCCCTG
NM_002566.4         TGGCACCCCGCCGTGGTCTTCTCTGTCCAGCTGGCAGTCAGCGACCTGCTCTGCGCCCTG
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      ACGCTGCCCCCGCTGGCCGCCTACCTCTATCCCCCCAAGCACTGGCGCTATGGGGAGGCC
NM_002566.4         ACGCTGCCCCCGCTGGCCGCCTACCTCTATCCCCCCAAGCACTGGCGCTATGGGGAGGCC
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      GCGTGCCGCCTGGAGCGCTTCCTCTTCACCTGCAACCTGCTGGGCAGCGTCATCTTCATC
NM_002566.4         GCGTGCCGCCTGGAGCGCTTCCTCTTCACCTGCAACCTGCTGGGCAGCGTCATCTTCATC
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      ACCTGCATCAGCCTCAACCGCTACCTGGGCATCGTGCACCCCTTCTTCGCCCGAAGCCAC
NM_002566.4         ACCTGCATCAGCCTCAACCGCTACCTGGGCATCGTGCACCCCTTCTTCGCCCGAAGCCAC
                                                                                

HsCD00507047        ------------------------------------------------------------
NM_001040664.2      CTGCGACCCAAGCACGCCTGGGCCGTGAGCGCTGCCGGCTGGGTCCTGGCCGCCCTGCTG
NM_002566.4         CTGCGACCCAAGCACGCCTGGGCCGTGAGCGCTGCCGGCTGGGTCCTGGCCGCCCTGCTG
                                                                                

HsCD00507047        ---ATGCCCACACTCAGCTTCTCCCACCTGAAGAGGCCGCAGCAGGGGGCGGGCAACTGC
NM_001040664.2      GCCATGCCCACACTCAGCTTCTCCCACCTGAAGAGGCCGCAGCAGGGGGCGGGCAACTGC
NM_002566.4         GCCATGCCCACACTCAGCTTCTCCCACCTGAAGAGGCCGCAGCAGGGGGCGGGCAACTGC
                       *********************************************************

HsCD00507047        AGCGTGGCCAGGCCCGAGGCCTGCATCAAGTGTCTGGGGACAGCAGACCACGGGCTGGCG
NM_001040664.2      AGCGTGGCCAGGCCCGAGGCCTGCATCAAGTGTCTGGGGACAGCAGACCACGGGCTGGCG
NM_002566.4         AGCGTGGCCAGGCCCGAGGCCTGCATCAAGTGTCTGGGGACAGCAGACCACGGGCTGGCG
                    ************************************************************

HsCD00507047        GCCTACAGAGCGTATAGCCTGGTGCTGGCGGGGTTGGGCTGCGGCCTGCCGCTGCTGCTC
NM_001040664.2      GCCTACAGAGCGTATAGCCTGGTGCTGGCGGGGTTGGGCTGCGGCCTGCCGCTGCTGCTC
NM_002566.4         GCCTACAGAGCGTATAGCCTGGTGCTGGCGGGGTTGGGCTGCGGCCTGCCGCTGCTGCTC
                    ************************************************************

HsCD00507047        ACGCTGGCAGCCTACGGCGCCCTCGGGCGGGCCGTGCTACGCAGCCCAGGCATGACTGTG
NM_001040664.2      ACGCTGGCAGCCTACGGCGCCCTCGGGCGGGCCGTGCTACGCAGCCCAGGCATGACTGTG
NM_002566.4         ACGCTGGCAGCCTACGGCGCCCTCGGGCGGGCCGTGCTACGCAGCCCAGGCATGACTGTG
                    ************************************************************

HsCD00507047        GCCGAGAAGCTGCGTGTGGCAGCGTTGGTGGCCAGTGGTGTGGCCCTCTACGCCAGCTCC
NM_001040664.2      GCCGAGAAGCTGCGTGTGGCAGCGTTGGTGGCCAGTGGTGTGGCCCTCTACGCCAGCTCC
NM_002566.4         GCCGAGAAGCTGCGTGTGGCAGCGTTGGTGGCCAGTGGTGTGGCCCTCTACGCCAGCTCC
                    ************************************************************

HsCD00507047        TATGTGCCCTACCACATCATGCGGGTGCTCAACGTGGATGCTCGGCGGCGCTGGAGCACC
NM_001040664.2      TATGTGCCCTACCACATCATGCGGGTGCTCAACGTGGATGCTCGGCGGCGCTGGAGCACC
NM_002566.4         TATGTGCCCTACCACATCATGCGGGTGCTCAACGTGGATGCTCGGCGGCGCTGGAGCACC
                    ************************************************************

HsCD00507047        CGCTGCCCGAGCTTTGCAGACATAGCCCAGGCCACAGCAGCCCTGGAGCTGGGGCCCTAC
NM_001040664.2      CGCTGCCCGAGCTTTGCAGACATAGCCCAGGCCACAGCAGCCCTGGAGCTGGGGCCCTAC
NM_002566.4         CGCTGCCCGAGCTTTGCAGACATAGCCCAGGCCACAGCAGCCCTGGAGCTGGGGCCCTAC
                    ************************************************************

HsCD00507047        GTGGGCTACCAGGTGATGCGGGGCCTCATGCCCCTGGCCTTCTGTGTCCACCCTCTACTC
NM_001040664.2      GTGGGCTACCAGGTGATGCGGGGCCTCATGCCCCTGGCCTTCTGTGTCCACCCTCTACTC
NM_002566.4         GTGGGCTACCAGGTGATGCGGGGCCTCATGCCCCTGGCCTTCTGTGTCCACCCTCTACTC
                    ************************************************************

HsCD00507047        TACATGGCCGCAGTGCCCAGCCTGGGCTGCTGCTGCCGACACTGCCCCGGCTACAGGGAC
NM_001040664.2      TACATGGCCGCAGTGCCCAGCCTGGGCTGCTGCTGCCGACACTGCCCCGGCTACAGGGAC
NM_002566.4         TACATGGCCGCAGTGCCCAGCCTGGGCTGCTGCTGCCGACACTGCCCCGGCTACAGGGAC
                    ************************************************************

HsCD00507047        AGCTGGAACCCAGAGGACGCCAAGAGCACTGGCCAAGCCCTGCCCCTCAATGCCACAGCC
NM_001040664.2      AGCTGGAACCCAGAGGACGCCAAGAGCACTGGCCAAGCCCTGCCCCTCAATGCCACAGCC
NM_002566.4         AGCTGGAACCCAGAGGACGCCAAGAGCACTGGCCAAGCCCTGCCCCTCAATGCCACAGCC
                    ************************************************************

HsCD00507047        GCCCCTAAACCGTCAGAGCCCCAGTCCCGTGAGCTGAGCCAA---
NM_001040664.2      GCCCCTAAACCGTCAGAGCCCCAGTCCCGTGAGCTGAGCCAATGA
NM_002566.4         GCCCCTAAACCGTCAGAGCCCCAGTCCCGTGAGCTGAGCCAATGA
                    ******************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (q) signalling events
Reactome : G alpha (s) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : GPCRs, Other

Clone

Cloning Information : 666796
Original Clone ID : ccsbBroadEn_11014

Organism


TAX_ID : 9606
Species Specific ID: 5032

Genome

Chromosome : 19
Map Location : 19p13
Ensembl : ENSG00000243207

Reagents

Labome : P2Y11-antibody