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LRRN4CL (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LRRN4CL
Gene Name:  LRRN4 C-terminal like
Sequence             
Original Clone ID: ccsbBroadEn_05250
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC053902.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00507742

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 715nts         Open reading frame : 1 to 715
Reference Sequence Alignment

ATGCTGGGCTCTCCCTGCCTTCTGTGGCTCCTGGCCGTGACCTTCTTGGTTCCCAGAGCT
CAGCCCTTGGCCCCTCAAGACTTTGAAGAAGAGGAGGCAGATGAGACTGAGACGGCGTGG
CCGCCTTTGCCGGCTGTCCCCTGCGACTACGACCACTGCCGACACCTGCAGGTGCCCTGC
AAGGAGCTACAGAGGGTCGGGCCGGCGGCCTGCCTGTGCCCAGGACTCTCCAGCCCCGCC
CAGCCGCCCGACCCGCCGCGCATGGGAGAAGTGCGCATTGCGGCCGAAGAGGGCCGCGCA
GTGGTCCACTGGTGTGCCCCCTTCTCCCCGGTCCTCCACTACTGGCTGCTGCTTTGGGAC
GGCAGCGAGGCTGCGCAGAAGGGGCCCCCGCTGAACGCTACGGTCCGCAGAGCCGAACTG
AAGGGGCTGAAGCCAGGGGGCATTTATGTCGTTTGCGTAGTGGCCGCTAACGAGGCCGGG
GCAAGCCGCGTGCCCCAGGCTGGAGGAGAGGGCCTCGAGGGGGCCGACATCCCTGCCTTC
GGGCCTTGCAGCCGCCTTGCGGTGCCGCCCAACCCCCGCACTCTGGTCCACGCGGCCGTC
GGGGTGGGCACGGCCCTGGCCCTGCTAAGCTGTGCCGCCCTGGTGTGGCACTTCTGCCTG
CGCGATCGCTGGGGCTGCCCGCGCCGAGCCGCCGCCCGAGCCGCAGGGGCGCTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LRRN4CL
Symbol Nomenclature : LRRN4CL
Designation : LRRN4 C-terminal-like protein
Full Nomenclature : LRRN4 C-terminal like
GENEID : 221091
Locus Tag : UNQ728/PRO1410
HGNC : 33724
Vega : OTTHUMG00000167581
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00507742      ATGCTGGGCTCTCCCTGCCTTCTGTGGCTCCTGGCCGTGACCTTCTTGGTTCCCAGAGCT
NM_203422.3       ATGCTGGGCTCTCCCTGCCTTCTGTGGCTCCTGGCCGTGACCTTCTTGGTTCCCAGAGCT
                  ************************************************************

HsCD00507742      CAGCCCTTGGCCCCTCAAGACTTTGAAGAAGAGGAGGCAGATGAGACTGAGACGGCGTGG
NM_203422.3       CAGCCCTTGGCCCCTCAAGACTTTGAAGAAGAGGAGGCAGATGAGACTGAGACGGCGTGG
                  ************************************************************

HsCD00507742      CCGCCTTTGCCGGCTGTCCCCTGCGACTACGACCACTGCCGACACCTGCAGGTGCCCTGC
NM_203422.3       CCGCCTTTGCCGGCTGTCCCCTGCGACTACGACCACTGCCGACACCTGCAGGTGCCCTGC
                  ************************************************************

HsCD00507742      AAGGAGCTACAGAGGGTCGGGCCGGCGGCCTGCCTGTGCCCAGGACTCTCCAGCCCCGCC
NM_203422.3       AAGGAGCTACAGAGGGTCGGGCCGGCGGCCTGCCTGTGCCCAGGACTCTCCAGCCCCGCC
                  ************************************************************

HsCD00507742      CAGCCGCCCGACCCGCCGCGCATGGGAGAAGTGCGCATTGCGGCCGAAGAGGGCCGCGCA
NM_203422.3       CAGCCGCCCGACCCGCCGCGCATGGGAGAAGTGCGCATTGCGGCCGAAGAGGGCCGCGCA
                  ************************************************************

HsCD00507742      GTGGTCCACTGGTGTGCCCCCTTCTCCCCGGTCCTCCACTACTGGCTGCTGCTTTGGGAC
NM_203422.3       GTGGTCCACTGGTGTGCCCCCTTCTCCCCGGTCCTCCACTACTGGCTGCTGCTTTGGGAC
                  ************************************************************

HsCD00507742      GGCAGCGAGGCTGCGCAGAAGGGGCCCCCGCTGAACGCTACGGTCCGCAGAGCCGAACTG
NM_203422.3       GGCAGCGAGGCTGCGCAGAAGGGGCCCCCGCTGAACGCTACGGTCCGCAGAGCCGAACTG
                  ************************************************************

HsCD00507742      AAGGGGCTGAAGCCAGGGGGCATTTATGTCGTTTGCGTAGTGGCCGCTAACGAGGCCGGG
NM_203422.3       AAGGGGCTGAAGCCAGGGGGCATTTATGTCGTTTGCGTAGTGGCCGCTAACGAGGCCGGG
                  ************************************************************

HsCD00507742      GCAAGCCGCGTGCCCCAGGCTGGAGGAGAGGGCCTCGAGGGGGCCGACATCCCTGCCTTC
NM_203422.3       GCAAGCCGCGTGCCCCAGGCTGGAGGAGAGGGCCTCGAGGGGGCCGACATCCCTGCCTTC
                  ************************************************************

HsCD00507742      GGGCCTTGCAGCCGCCTTGCGGTGCCGCCCAACCCCCGCACTCTGGTCCACGCGGCCGTC
NM_203422.3       GGGCCTTGCAGCCGCCTTGCGGTGCCGCCCAACCCCCGCACTCTGGTCCACGCGGCCGTC
                  ************************************************************

HsCD00507742      GGGGTGGGCACGGCCCTGGCCCTGCTAAGCTGTGCCGCCCTGGTGTGGCACTTCTGCCTG
NM_203422.3       GGGGTGGGCACGGCCCTGGCCCTGCTAAGCTGTGCCGCCCTGGTGTGGCACTTCTGCCTG
                  ************************************************************

HsCD00507742      CGCGATCGCTGGGGCTGCCCGCGCCGAGCCGCCGCCCGAGCCGCAGGGGCGCTC---
NM_203422.3       CGCGATCGCTGGGGCTGCCCGCGCCGAGCCGCCGCCCGAGCCGCAGGGGCGCTCTGA
                  ******************************************************   

Protein

SMART domain : FN3 : Fibronectin type 3 domain
HPRD : 17322

Clone

Cloning Information : 666064
Original Clone ID : ccsbBroadEn_05250

Organism


TAX_ID : 9606
Species Specific ID: 221091

Genome

Chromosome : 11
Map Location : 11q12.3
Ensembl : ENSG00000177363

Reagents

Labome : LRRN4CL-antibody