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APIP (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  APIP
Gene Name:  APAF1 interacting protein
Sequence             
Original Clone ID: ccsbBroadEn_03193
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC009077.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00507764

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 727nts         Open reading frame : 1 to 727
Reference Sequence Alignment

ATGTCTGGCTGTGATGCTCGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTGTGATATAAATGAA
AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
GCTGTGATGGCCACACTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGGA
ATTGTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : APIP
Symbol Nomenclature : APIP
SYNONYM : APIP2; CGI29; MMRP19; hAPIP
Designation : APAF1-interacting protein|MTRu-1-P dehydratase|methylthioribulose-1-phosphate dehydratase|probable methylthioribulose-1-phosphate dehydratase
Full Nomenclature : APAF1 interacting protein
GENEID : 51074
Locus Tag : CGI-29
GenBank Accession : HQ448158
HGNC : 17581
MIM : 612491
Vega : OTTHUMG00000166455
Target GenBank: HQ448158

 

Reference Sequence Alignment

  

HsCD00507764      ATGTCTGGCTGTGATGCTCGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
NM_015957.3       ATGTCTGGCTGTGATGCTCGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
                  ************************************************************

HsCD00507764      AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
NM_015957.3       AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
                  ************************************************************

HsCD00507764      GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
NM_015957.3       GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
                  ************************************************************

HsCD00507764      TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTGTGATATAAATGAA
NM_015957.3       TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTGTGATATAAATGAA
                  ************************************************************

HsCD00507764      AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
NM_015957.3       AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
                  ************************************************************

HsCD00507764      TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
NM_015957.3       TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
                  ************************************************************

HsCD00507764      GCTGTGATGGCCACACTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
NM_015957.3       GCTGTGATGGCCACACTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
                  ************************************************************

HsCD00507764      ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
NM_015957.3       ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
                  ************************************************************

HsCD00507764      GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
NM_015957.3       GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
                  ************************************************************

HsCD00507764      ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
NM_015957.3       ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
                  ************************************************************

HsCD00507764      GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
NM_015957.3       GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
                  ************************************************************

HsCD00507764      GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGGA
NM_015957.3       GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGGA
                  ************************************************************

HsCD00507764      ATTGTC---
NM_015957.3       ATTGTCTAA
                  ******   

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Clone

Cloning Information : 612231
Original Clone ID : ccsbBroadEn_03193

Organism


TAX_ID : 9606
Species Specific ID: 51074

Genome

Chromosome : 11
Map Location : 11p13
Ensembl : ENSG00000149089

Reagents

Labome : APIP-antibody