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HTATIP2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HTATIP2
Gene Name:  HIV-1 Tat interactive protein 2, 30kDa
Sequence             
Original Clone ID: ccsbBroadEn_07633
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC002439.2,BC015358.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00507795

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 727nts         Open reading frame : 1 to 727
Reference Sequence Alignment

ATGGCCGAAACAGAAGCCCTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCC
GTCTTTATTTTGGGCGCCAGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAG
CAGGGCCTGTTTTCCAAAGTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAA
GCTTATAAAAATGTGAATCAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCT
GCCTTTCAAGGTCATGATGTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGG
GCGGAGGGATTTGTTCGTGTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAA
GCTGGAGGGTGCAAACATTTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAAT
TTTTTATATCTACAAGTTAAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGAT
CGTTACTCTGTATTTAGGCCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGT
GAATGGCTGGTTAGAAAGTTCTTTGGCTCCTTACCAGACTCTTGGGCCAGGGGGCATTCT
GTGCCTGTGGTGACCGTGGTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAG
CAGATGGAACTGCTGGAGAACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTC
AAGCCA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HTATIP2
Symbol Nomenclature : HTATIP2
SYNONYM : CC3; SDR44U1; TIP30
Designation : 30 kDa HIV-1 TAT-interacting protein|HIV-1 TAT-interactive protein 2|Tat-interacting protein (30kD)|oxidoreductase HTATIP2|short chain dehydrogenase/reductase family 44U, member 1
Full Nomenclature : HIV-1 Tat interactive protein 2, 30kDa
GENEID : 10553
GenBank Accession : EU446982
HGNC : 16637
MIM : 605628
Vega : OTTHUMG00000166015
Target GenBank: EU446982

 

Reference Sequence Alignment

  

HsCD00507795        ------------------------------------------------------------
NM_001098522.1      ------------------------------------------------------------
NM_001098521.1      ------------------------------------------------------------
NM_001098520.1      ATGGCCGGGCCTGCGGCGCTGAGCGCGGCGGCGGCGGCTGCTCTGGCGGCCGCCCTGCTC
NM_006410.4         ------------------------------------------------------------
                                                                                

HsCD00507795        ------------------------------------------ATGGCCGAAACAGAAGCC
NM_001098522.1      ------------------------------------------ATGGCCGAAACAGAAGCC
NM_001098521.1      ------------------------------------------ATGGCCGAAACAGAAGCC
NM_001098520.1      CTGCTGCGTCGTGAGGACCCGGGGCCGGGGGCTGGCCCCAGCATGGCCGAAACAGAAGCC
NM_006410.4         ------------------------------------------ATGGCCGAAACAGAAGCC
                                                              ******************

HsCD00507795        CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_001098522.1      CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_001098521.1      CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_001098520.1      CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
NM_006410.4         CTGTCGAAGCTTCGGGAAGACTTCAGGATGCAGAATAAATCCGTCTTTATTTTGGGCGCC
                    ************************************************************

HsCD00507795        AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_001098522.1      AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_001098521.1      AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_001098520.1      AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
NM_006410.4         AGCGGAGAAACCGGCAGAGTGCTCTTAAAGGAAATCCTGGAGCAGGGCCTGTTTTCCAAA
                    ************************************************************

HsCD00507795        GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_001098522.1      GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_001098521.1      GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_001098520.1      GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
NM_006410.4         GTCACGCTCATTGGCCGGAGGAAGCTCACCTTCGACGAGGAAGCTTATAAAAATGTGAAT
                    ************************************************************

HsCD00507795        CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_001098522.1      CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_001098521.1      CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_001098520.1      CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
NM_006410.4         CAAGAAGTGGTGGACTTTGAAAAGTTGGATGACTACGCCTCTGCCTTTCAAGGTCATGAT
                    ************************************************************

HsCD00507795        GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_001098522.1      GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_001098521.1      GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_001098520.1      GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
NM_006410.4         GTTGGATTCTGTTGCCTGGGTACCACCAGAGGGAAAGCTGGGGCGGAGGGATTTGTTCGT
                    ************************************************************

HsCD00507795        GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_001098522.1      GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_001098521.1      GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_001098520.1      GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
NM_006410.4         GTTGACCGAGATTATGTGCTGAAGTCTGCAGAGCTGGCAAAAGCTGGAGGGTGCAAACAT
                    ************************************************************

HsCD00507795        TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_001098522.1      TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_001098521.1      TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_001098520.1      TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
NM_006410.4         TTCAACTTGCTATCCTCTAAAGGAGCTGATAAATCAAGCAATTTTTTATATCTACAAGTT
                    ************************************************************

HsCD00507795        AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_001098522.1      AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_001098521.1      AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_001098520.1      AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
NM_006410.4         AAGGGAGAAGTAGAAGCCAAGGTTGAAGAATTAAAATTTGATCGTTACTCTGTATTTAGG
                    ************************************************************

HsCD00507795        CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_001098522.1      CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_001098521.1      CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_001098520.1      CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
NM_006410.4         CCTGGAGTTCTGTTATGTGATAGGCAAGAATCTCGCCCAGGTGAATGGCTGGTTAGAAAG
                    ************************************************************

HsCD00507795        TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGGGGGCATTCTGTGCCTGTGGTGACCGTG
NM_001098522.1      TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
NM_001098521.1      TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
NM_001098520.1      TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
NM_006410.4         TTCTTTGGCTCCTTACCAGACTCTTGGGCCAGTGGGCATTCTGTGCCTGTGGTGACCGTG
                    ******************************** ***************************

HsCD00507795        GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_001098522.1      GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_001098521.1      GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_001098520.1      GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
NM_006410.4         GTTAGAGCAATGCTGAACAATGTGGTGAGACCAAGAGACAAGCAGATGGAACTGCTGGAG
                    ************************************************************

HsCD00507795        AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCA---
NM_001098522.1      AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
NM_001098521.1      AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
NM_001098520.1      AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
NM_006410.4         AACAAGGCCATCCATGACCTGGGGAAAGCGCATGGCTCTCTCAAGCCATGA
                    ************************************************   

Clone

Cloning Information : 612262
Original Clone ID : ccsbBroadEn_07633

Organism


TAX_ID : 9606
Species Specific ID: 10553

Genome

Chromosome : 11
Map Location : 11p15.1
Ensembl : ENSG00000109854

Reagents

Labome : TIP30-antibody