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FGF13 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF13
Gene Name:  fibroblast growth factor 13
Sequence             
Original Clone ID: ccsbBroadEn_00559
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC012347.1,BC034340.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00507849

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 736nts         Open reading frame : 1 to 736
Reference Sequence Alignment

ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGAG
AAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAAA
AACAAGTTAAATGTCTTTTCCCGGGTCAAACTCTTCGGCTCCAAGAAGAGGCGCAGAAGA
AGACCAGAGCCTCAGCTTAAGGGTATAGTTACCAAGCTATACAGCCGACAAGGCTACCAC
TTGCAGCTGCAGGCGGATGGAACCATTGATGGCACCAAAGATGAGGACAGCACTTACACT
CTGTTTAACCTCATCCCTGTGGGTCTGCGAGTGGTGGCTATCCAAGGAGTTCAAACCAAG
CTGTACTTGGCAATGAACAGTGAGGGATACTTGTACACCTCGGAACTTTTCACACCTGAG
TGCAAATTCAAAGAATCAGTGTTTGAAAATTATTATGTGACATATTCATCAATGATATAC
CGTCAGCAGCAGTCAGGCCGAGGGTGGTATCTGGGTCTGAACAAAGAAGGAGAGATCATG
AAAGGCAACCATGTGAAGAAGAACAAGCCTGCAGCTCATTTTCTGCCTAAACCACTGAAA
GTGGCCATGTACAAGGAGCCATCACTGCACGATCTCACGGAGTTCTCCCGATCTGGAAGC
GGGACCCCAACCAAGAGCAGAAGTGTCTCTGGCGTGCTGAACGGAGGCAAATCCATGAGC
CACAATGAATCAACG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF13
Symbol Nomenclature : FGF13
SYNONYM : FGF-13; FGF2; FHF-2; FHF2
Designation : fibroblast growth factor homologous factor 2
Full Nomenclature : fibroblast growth factor 13
GENEID : 2258
Locus Tag : RP6-27P15.3
GenBank Accession : DQ895746
HGNC : 3670
MIM : 300070
Vega : OTTHUMG00000022532
Target GenBank: DQ895746

 

Reference Sequence Alignment

  

HsCD00507849        -ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGA
NM_001139501.1      ------------------------------------------------------------
XM_005262399.1      ATGAGTGGAAAGGTGACCAAGCCCAAAGAGGAGAAAGATGCTTCTAAGGTTCTGGATGAC
NM_004114.3         -ATGGCGGCGGCTATCGCCAGCTCGCTCATCCGTCAGAAGAGGCAAGCCCGCGAGCGCGA
NM_001139502.1      ------------------------------------------------------------
NM_001139500.1      ATGAGTGGAAAGGTGACCAAGCCCAAAGAGGAGAAAGATGCTTCTAAGGTTCTGGATGAC
                                                                                

HsCD00507849        GAAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAA
NM_001139501.1      ---------------------------ATGTTACGACAAGATTCCATCCAATCTGCGGAA
XM_005262399.1      GCCCCCCCTGGCACACAGGAATACATTATGTTACGACAAGATTCCATCCAATCTGCGGAA
NM_004114.3         GAAATCCAACGCCTGCAAGTGTGTCAGCAGCCCCAGCAAAGGCAAGACCAGCTGCGACAA
NM_001139502.1      ---------------------------ATGTTACGACAAGATTCCATCCAATCTGCGGAA
NM_001139500.1      GCCCCCCCTGGCACACAGGAATACATTATGTTACGACAAGATTCCATCCAATCTGCGGAA
                                               .:*  .*..***..  ...:***.     . **

HsCD00507849        AAACAAGTTAAATG-----------------------------TCTTTTCCCGGGTCAAA
NM_001139501.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
XM_005262399.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
NM_004114.3         AAACAAGTTAAATG-----------------------------TCTTTTCCCGGGTCAAA
NM_001139502.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
NM_001139500.1      TTAAAGAAAAAAGAGTCCCCCTTTCGTGCTAAGTGTCACGAAATCTTCTGCTGCCCGCTG
                    ::*.*..::*** .                             **** * * *    .:.

HsCD00507849        CTCTTCGGCTCCAAGAAGAGGCGCAGAAGAAGACCAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139501.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
XM_005262399.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
NM_004114.3         CTCTTCGGCTCCAAGAAGAGGCGCAGAAGAAGACCAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139502.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
NM_001139500.1      AAGCAAGTACACCACAAAGAGAACACAGAGCCGGAAGAGCCTCAGCTTAAGGGTATAGTT
                    .:  :.* . .*.* **...*..** *.... . .*************************

HsCD00507849        ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139501.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
XM_005262399.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_004114.3         ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139502.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
NM_001139500.1      ACCAAGCTATACAGCCGACAAGGCTACCACTTGCAGCTGCAGGCGGATGGAACCATTGAT
                    ************************************************************

HsCD00507849        GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139501.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
XM_005262399.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_004114.3         GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139502.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
NM_001139500.1      GGCACCAAAGATGAGGACAGCACTTACACTCTGTTTAACCTCATCCCTGTGGGTCTGCGA
                    ************************************************************

HsCD00507849        GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139501.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
XM_005262399.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_004114.3         GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139502.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
NM_001139500.1      GTGGTGGCTATCCAAGGAGTTCAAACCAAGCTGTACTTGGCAATGAACAGTGAGGGATAC
                    ************************************************************

HsCD00507849        TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139501.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
XM_005262399.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_004114.3         TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139502.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
NM_001139500.1      TTGTACACCTCGGAACTTTTCACACCTGAGTGCAAATTCAAAGAATCAGTGTTTGAAAAT
                    ************************************************************

HsCD00507849        TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139501.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
XM_005262399.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_004114.3         TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139502.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
NM_001139500.1      TATTATGTGACATATTCATCAATGATATACCGTCAGCAGCAGTCAGGCCGAGGGTGGTAT
                    ************************************************************

HsCD00507849        CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139501.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
XM_005262399.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_004114.3         CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139502.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
NM_001139500.1      CTGGGTCTGAACAAAGAAGGAGAGATCATGAAAGGCAACCATGTGAAGAAGAACAAGCCT
                    ************************************************************

HsCD00507849        GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139501.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
XM_005262399.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_004114.3         GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139502.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
NM_001139500.1      GCAGCTCATTTTCTGCCTAAACCACTGAAAGTGGCCATGTACAAGGAGCCATCACTGCAC
                    ************************************************************

HsCD00507849        GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139501.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
XM_005262399.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_004114.3         GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139502.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
NM_001139500.1      GATCTCACGGAGTTCTCCCGATCTGGAAGCGGGACCCCAACCAAGAGCAGAAGTGTCTCT
                    ************************************************************

HsCD00507849        GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACG---
NM_001139501.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
XM_005262399.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_004114.3         GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139502.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
NM_001139500.1      GGCGTGCTGAACGGAGGCAAATCCATGAGCCACAATGAATCAACGTAG
                    *********************************************   

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Cloning Information : 613068
Original Clone ID : ccsbBroadEn_00559

Organism


TAX_ID : 9606
Species Specific ID: 2258

Genome

Chromosome : X
Map Location : Xq26.3
Ensembl : ENSG00000129682

Reagents

Labome : FGF13-antibody