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PPP6C (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PPP6C
Gene Name:  protein phosphatase 6, catalytic subunit
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH019818.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000508

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 918nts         Open reading frame : 1 to 918
Reference Sequence Alignment

ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGAC
CTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCCTGACACAAACTACATATTTATGGGT
GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
GATGTGGACACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
ACGCCATATTTCCTTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 939
Start on reference sequence 22
Mutations:
None
Discrepancy:
t609c
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPP6C
Symbol Nomenclature : PPP6C
SYNONYM : PP6; PP6C
Designation : serine/threonine protein phosphatase catalytic subunit|serine/threonine-protein phosphatase 6 catalytic subunit
Full Nomenclature : protein phosphatase 6, catalytic subunit
GENEID : 5537
Locus Tag : RP11-366O20.1
GI : 61365951
GenBank Accession : AY890852
HGNC : 9323
MIM : 612725
Vega : OTTHUMG00000020671
Target GenBank: NM_002721

 

Reference Sequence Alignment

  

HsCD00000508        ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
NM_002721.4         ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
NM_001123369.1      ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
                    ************************************************************

HsCD00000508        GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
NM_002721.4         GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
NM_001123369.1      GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
                    ************************************************************

HsCD00000508        GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGAC
NM_002721.4         GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGAC
NM_001123369.1      GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATG--------------
                    **********************************************              

HsCD00000508        CTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCCTGACACAAACTACATATTTATGGGT
NM_002721.4         CTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCCTGACACAAACTACATATTTATGGGT
NM_001123369.1      ----------------------------------------------------GACAGGGT
                                                                         :.:****

HsCD00000508        GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
NM_002721.4         GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
NM_001123369.1      GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
                    ************************************************************

HsCD00000508        GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
NM_002721.4         GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
NM_001123369.1      GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
                    ************************************************************

HsCD00000508        CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
NM_002721.4         CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
NM_001123369.1      CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
                    ************************************************************

HsCD00000508        TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
NM_002721.4         TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
NM_001123369.1      TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
                    ************************************************************

HsCD00000508        TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
NM_002721.4         TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
NM_001123369.1      TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
                    ************************************************************

HsCD00000508        CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
NM_002721.4         CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
NM_001123369.1      CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
                    ************************************************************

HsCD00000508        GATGTGGACACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
NM_002721.4         GATGTGGATACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
NM_001123369.1      GATGTGGATACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
                    ******** ***************************************************

HsCD00000508        ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
NM_002721.4         ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
NM_001123369.1      ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
                    ************************************************************

HsCD00000508        CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
NM_002721.4         CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
NM_001123369.1      CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
                    ************************************************************

HsCD00000508        TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
NM_002721.4         TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
NM_001123369.1      TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
                    ************************************************************

HsCD00000508        GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
NM_002721.4         GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
NM_001123369.1      GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
                    ************************************************************

HsCD00000508        ACGCCATATTTCCTTTTG
NM_002721.4         ACGCCATATTTCCTTTGA
NM_001123369.1      ACGCCATATTTCCTTTGA
                    **************** .

Clone

Cloning Information : 19818
HIP Master Clone ID : 34328
Original Clone ID : FLH019818.01L

Organism


TAX_ID : 9606
Species Specific ID: 5537

Genome

Chromosome : 9
Map Location : 9q33.3
Ensembl : ENSG00000119414

Reagents

Labome : PPP6C-antibody
Labome : PPP6C-siRNA