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FGF6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF6
Gene Name:  fibroblast growth factor 6
Sequence             
Original Clone ID: ccsbBroadEn_06207
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC121097.1
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00508372

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 625nts         Open reading frame : 1 to 625
Reference Sequence Alignment

ATGGCCCTGGGACAGAAACTGTTCATCACTATGTCCCGGGGAGCAGGACGTCTGCAGGGC
ACGCTGTGGGCTCTCGTCTTCCTAGGCATCCTAGTGGGCATGGTGGTGCCCTCGCCTGCA
GGCACCCGTGCCAACAACACGCTGCTGGACTCGAGGGGCTGGGGCACCCTGCTGTCCAGG
TCTCGCGCGGGGCTAGCTGGAGAGATTGCCGGGGTGAACTGGGAAAGTGGCTATTTGGTG
GGGATCAAGCGGCAGCGGAGGCTCTACTGCAACGTGGGCATCGGCTTTCACCTCCAGGTG
CTCCCCGACGGCCGGATCAGCGGGACCCACGAGGAGAACCCCTACAGCCTGCTGGAAATT
TCCACCGTGGAGCGAGGCGTGGTGAGTCTCTTTGGAGTGAGAAGTGCCCTCTTCGTCGCC
ATGAACAGTAAAGGAAGATTGTACGCAACGCCCAGCTTCCAAGAAGAATGCAAGTTCAGA
GAAACCCTCCTGCCCAACAATTACAATGCCTACGAGTCAGACTTGTACCAAGGGACCTAC
ATTGCCCTGAGCAAATACGGACGGGTAAAGCGGGGCAGCAAGGTGTCCCCGATCATGACT
GTCACTCATTTCCTTCCCAGGATC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF6
Symbol Nomenclature : FGF6
SYNONYM : HBGF-6; HST2
Designation : FGF-6|HST-2|HSTF-2|heparin secretory-transforming protein 2|heparin-binding growth factor 6
Full Nomenclature : fibroblast growth factor 6
GENEID : 2251
HGNC : 3684
MIM : 134921
Vega : OTTHUMG00000168242
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00508372      ATGGCCCTGGGACAGAAACTGTTCATCACTATGTCCCGGGGAGCAGGACGTCTGCAGGGC
NM_020996.1       ATGGCCCTGGGACAGAAACTGTTCATCACTATGTCCCGGGGAGCAGGACGTCTGCAGGGC
                  ************************************************************

HsCD00508372      ACGCTGTGGGCTCTCGTCTTCCTAGGCATCCTAGTGGGCATGGTGGTGCCCTCGCCTGCA
NM_020996.1       ACGCTGTGGGCTCTCGTCTTCCTAGGCATCCTAGTGGGCATGGTGGTGCCCTCGCCTGCA
                  ************************************************************

HsCD00508372      GGCACCCGTGCCAACAACACGCTGCTGGACTCGAGGGGCTGGGGCACCCTGCTGTCCAGG
NM_020996.1       GGCACCCGTGCCAACAACACGCTGCTGGACTCGAGGGGCTGGGGCACCCTGCTGTCCAGG
                  ************************************************************

HsCD00508372      TCTCGCGCGGGGCTAGCTGGAGAGATTGCCGGGGTGAACTGGGAAAGTGGCTATTTGGTG
NM_020996.1       TCTCGCGCGGGGCTAGCTGGAGAGATTGCCGGGGTGAACTGGGAAAGTGGCTATTTGGTG
                  ************************************************************

HsCD00508372      GGGATCAAGCGGCAGCGGAGGCTCTACTGCAACGTGGGCATCGGCTTTCACCTCCAGGTG
NM_020996.1       GGGATCAAGCGGCAGCGGAGGCTCTACTGCAACGTGGGCATCGGCTTTCACCTCCAGGTG
                  ************************************************************

HsCD00508372      CTCCCCGACGGCCGGATCAGCGGGACCCACGAGGAGAACCCCTACAGCCTGCTGGAAATT
NM_020996.1       CTCCCCGACGGCCGGATCAGCGGGACCCACGAGGAGAACCCCTACAGCCTGCTGGAAATT
                  ************************************************************

HsCD00508372      TCCACCGTGGAGCGAGGCGTGGTGAGTCTCTTTGGAGTGAGAAGTGCCCTCTTCGTCGCC
NM_020996.1       TCCACTGTGGAGCGAGGCGTGGTGAGTCTCTTTGGAGTGAGAAGTGCCCTCTTCGTTGCC
                  ***** ************************************************** ***

HsCD00508372      ATGAACAGTAAAGGAAGATTGTACGCAACGCCCAGCTTCCAAGAAGAATGCAAGTTCAGA
NM_020996.1       ATGAACAGTAAAGGAAGATTGTACGCAACGCCCAGCTTCCAAGAAGAATGCAAGTTCAGA
                  ************************************************************

HsCD00508372      GAAACCCTCCTGCCCAACAATTACAATGCCTACGAGTCAGACTTGTACCAAGGGACCTAC
NM_020996.1       GAAACCCTCCTGCCCAACAATTACAATGCCTACGAGTCAGACTTGTACCAAGGGACCTAC
                  ************************************************************

HsCD00508372      ATTGCCCTGAGCAAATACGGACGGGTAAAGCGGGGCAGCAAGGTGTCCCCGATCATGACT
NM_020996.1       ATTGCCCTGAGCAAATACGGACGGGTAAAGCGGGGCAGCAAGGTGTCCCCGATCATGACT
                  ************************************************************

HsCD00508372      GTCACTCATTTCCTTCCCAGGATC---
NM_020996.1       GTCACTCATTTCCTTCCCAGGATCTAA
                  ************************   

Pathways

NCI : FGF signaling pathway
NCI : Syndecan-4-mediated signaling events
Panther : FGF signaling pathway
Reactome : Activated point mutants of FGFR2
Reactome : Adaptive Immune System
Reactome : Constitutive PI3K/AKT Signaling in Cancer
Reactome : DAP12 interactions
Reactome : DAP12 signaling
Reactome : Disease
Reactome : Downstream signal transduction
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : Downstream signaling of activated FGFR
Reactome : FGFR ligand binding and activation
Reactome : FGFR1 ligand binding and activation
Reactome : FGFR1c ligand binding and activation
Reactome : FGFR2 ligand binding and activation
Reactome : FGFR2c ligand binding and activation
Reactome : FGFR4 ligand binding and activation
Reactome : FRS2-mediated cascade
Reactome : Fc epsilon receptor (FCERI) signaling
Reactome : GAB1 signalosome
Reactome : IGF1R signaling cascade
Reactome : IRS-mediated signalling
Reactome : IRS-related events
Reactome : IRS-related events triggered by IGF1R
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Insulin receptor signalling cascade
Reactome : NGF signalling via TRKA from the plasma membrane
Reactome : Negative regulation of FGFR signaling
Reactome : PI-3K cascade
Reactome : PI3K Cascade
Reactome : PI3K events in ERBB2 signaling
Reactome : PI3K events in ERBB4 signaling
Reactome : PI3K/AKT Signaling in Cancer
Reactome : PI3K/AKT activation
Reactome : PIP3 activates AKT signaling
Reactome : Phospholipase C-mediated cascade
Reactome : Role of LAT2/NTAL/LAB on calcium mobilization
Reactome : SHC-mediated cascade
Reactome : Signal Transduction
Reactome : Signaling by EGFR
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by ERBB4
Reactome : Signaling by FGFR
Reactome : Signaling by FGFR in disease
Reactome : Signaling by FGFR mutants
Reactome : Signaling by FGFR1 mutants
Reactome : Signaling by FGFR2 mutants
Reactome : Signaling by Insulin receptor
Reactome : Signaling by PDGF
Reactome : Signaling by SCF-KIT
Reactome : Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome : Signaling by activated point mutants of FGFR1
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : Signalling by NGF
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Cloning Information : 666759
Original Clone ID : ccsbBroadEn_06207

Organism


TAX_ID : 9606
Species Specific ID: 2251

Genome

Chromosome : 12
Map Location : 12p13
Ensembl : ENSG00000111241

Reagents

Labome : FGF6-antibody