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ARHGEF38 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ARHGEF38
Gene Name:  Rho guanine nucleotide exchange factor (GEF) 38
Sequence             
Original Clone ID: ccsbBroadEn_03467
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC105695.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00508385

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 658nts         Open reading frame : 1 to 658
Reference Sequence Alignment

ATGGAGCCCAAAGAAGCCACTGGGAAAGAAAACATGGTCACCAAGAAAAAGAATCTGGCC
TTCTTGAGGTCTAGACTCTATATGCTGGAGAGAAGGAAGACTGACACTGTGGTTGAGAGC
AGTGTTTCTGGGGACCACTCTGGCACCTTGAGGAGGAGCCAATCTGACAGGACCGAATAC
AACCAGAAATTACAAGAAAAGATGACTCCACAGGGTGAGTGTTCTGTAGCTGAGACCTTA
ACCCCAGAGGAAGAGCATCATATGAAGAGGATGATGGCAAAGCGGGAAAAGATCATTAAG
GAGCTGATACAGACAGAAAAGGATTATCTCAATGATCTAGAGCTGTGTGTTAGGGAAGTG
GTTCAGCCCCTGAGAAATAAAAAGACTGATAGGCTGGATGTGGATAGCTTGTTTAGCAAC
ATTGAGTCCGTGCATCAGATATCAGCCAAGCTGCTGTCATTGTTGGAAGAGGCCACAACA
GACGTGGAACCGGCCATGCAAGTAATTGGAGAAGTATTCTTGCAGATTAAAGGGCCACTG
GAAGATATTTATAAAATCTACTGCTATCACCATGATGAAGCACATAGTATACTGGAGTCC
TATGAAAAGGAAGAAGAGCTGAAGGAACATTTGAGCCACTGTATCCAGTCCTTAAAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARHGEF38
Symbol Nomenclature : ARHGEF38
Designation : rho guanine nucleotide exchange factor 38
Full Nomenclature : Rho guanine nucleotide exchange factor (GEF) 38
GENEID : 54848
HGNC : 25968
Vega : OTTHUMG00000154752
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00508385        ATGGAGCCCAAAGAAGCCACTGGGAAAGAAAACATGGTCACCAAGAAAAAGAATCTGGCC
NM_001242729.1      ATGGAGCCCAAAGAAGCCACTGGGAAAGAAAACATGGTCACCAAGAAAAAGAATCTGGCC
NM_017700.1         ATGGAGCCCAAAGAAGCCACTGGGAAAGAAAACATGGTCACCAAGAAAAAGAATCTGGCC
                    ************************************************************

HsCD00508385        TTCTTGAGGTCTAGACTCTATATGCTGGAGAGAAGGAAGACTGACACTGTGGTTGAGAGC
NM_001242729.1      TTCTTGAGGTCTAGACTCTATATGCTGGAGAGAAGGAAGACTGACACTGTGGTTGAGAGC
NM_017700.1         TTCTTGAGGTCTAGACTCTATATGCTGGAGAGAAGGAAGACTGACACTGTGGTTGAGAGC
                    ************************************************************

HsCD00508385        AGTGTTTCTGGGGACCACTCTGGCACCTTGAGGAGGAGCCAATCTGACAGGACCGAATAC
NM_001242729.1      AGTGTTTCTGGGGACCACTCTGGCACCTTGAGGAGGAGCCAATCTGACAGGACCGAATAC
NM_017700.1         AGTGTTTCTGGGGACCACTCTGGCACCTTGAGGAGGAGCCAATCTGACAGGACCGAATAC
                    ************************************************************

HsCD00508385        AACCAGAAATTACAAGAAAAGATGACTCCACAGGGTGAGTGTTCTGTAGCTGAGACCTTA
NM_001242729.1      AACCAGAAATTACAAGAAAAGATGACTCCACAGGGTGAGTGTTCTGTAGCTGAGACCTTA
NM_017700.1         AACCAGAAATTACAAGAAAAGATGACTCCACAGGGTGAGTGTTCTGTAGCTGAGACCTTA
                    ************************************************************

HsCD00508385        ACCCCAGAGGAAGAGCATCATATGAAGAGGATGATGGCAAAGCGGGAAAAGATCATTAAG
NM_001242729.1      ACCCCAGAGGAAGAGCATCATATGAAGAGGATGATGGCAAAGCGGGAAAAGATCATTAAG
NM_017700.1         ACCCCAGAGGAAGAGCATCATATGAAGAGGATGATGGCAAAGCGGGAAAAGATCATTAAG
                    ************************************************************

HsCD00508385        GAGCTGATACAGACAGAAAAGGATTATCTCAATGATCTAGAGCTGTGTGTTAGGGAAGTG
NM_001242729.1      GAGCTGATACAGACAGAAAAGGATTATCTCAATGATCTAGAGCTGTGTGTTAGGGAAGTG
NM_017700.1         GAGCTGATACAGACAGAAAAGGATTATCTCAATGATCTAGAGCTGTGTGTTAGGGAAGTG
                    ************************************************************

HsCD00508385        GTTCAGCCCCTGAGAAATAAAAAGACTGATAGGCTGGATGTGGATAGCTTGTTTAGCAAC
NM_001242729.1      GTTCAGCCCCTGAGAAATAAAAAGACTGATAGGCTGGATGTGGATAGCTTGTTTAGCAAC
NM_017700.1         GTTCAGCCCCTGAGAAATAAAAAGACTGATAGGCTGGATGTGGATAGCTTGTTTAGCAAC
                    ************************************************************

HsCD00508385        ATTGAGTCCGTGCATCAGATATCAGCCAAGCTGCTGTCATTGTTGGAAGAGGCCACAACA
NM_001242729.1      ATTGAGTCCGTGCATCAGATATCAGCCAAGCTGCTGTCATTGTTGGAAGAGGCCACAACA
NM_017700.1         ATTGAGTCCGTGCATCAGATATCAGCCAAGCTGCTGTCATTGTTGGAAGAGGCCACAACA
                    ************************************************************

HsCD00508385        GACGTGGAACCGGCCATGCAAGTAATTGGAGAAGTATTCTTGCAGATTAAAGGGCCACTG
NM_001242729.1      GACGTGGAACCGGCCATGCAAGTAATTGGAGAAGTATTCTTGCAGATTAAAGGGCCACTG
NM_017700.1         GACGTGGAACCGGCCATGCAAGTAATTGGAGAAGTATTCTTGCAGATTAAAGGGCCACTG
                    ************************************************************

HsCD00508385        GAAGATATTTATAAAATCTACTGCTATCACCATGATGAAGCACATAGTATACTGGAGTCC
NM_001242729.1      GAAGATATTTATAAAATCTACTGCTATCACCATGATGAAGCACATAGTATACTGGAGTCC
NM_017700.1         GAAGATATTTATAAAATCTACTGCTATCACCATGATGAAGCACATAGTATACTGGAGTCC
                    ************************************************************

HsCD00508385        TATGAAAAGGAAGAAGAGCTGAAGGAACATTTGAGCCACTGTATCCAGTCCTTAAAG---
NM_001242729.1      TATGAAAAGGAAGAAGAGCTGAAGGAACATTTGAGCCACTGTATCCAGTCCTTAAAGAAA
NM_017700.1         TATGAAAAGGAAGAAGAGCTGAAGGAACATTTGAGCCACTGTATCCAGTCCTTAAAGTAA
                    *********************************************************   

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      ATATACATGCAAGAAGGCAAACCAAACTTATTGGACATGGGCTCTTTGATGATCAAACCA
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      ATTCAACGTGTGATGAAATACCCCCTATTACTGTGCGAACTTCGGAATTCCACCCCTCCC
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      TCTCACCCAGATTACAGAGCACTGGACGATGCCTTTGCTGCTGTGAAGGACATTAATGTT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      AACATCAATGAACTTAAAAGAAGGAAAGATTTAGTTCTAAAATACAAGAAGAATGACGAG
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      GATGAATCACTTAAAGACAAATTGTCTAAACTAAATATTCATTCAATTAGCAAGAAATCA
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      AAAAGAGTGACAAATCATCTGAAGATTCTGACCAGAGGAGAATCACAGGTTAAAGACAAT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      ACCTTTAACAGAGAAGAAAAGCTGTTTAGAGCTTTAGAAAAGACTGTGAGGCTTTGTGTG
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      AAGAACATTTCACTCTGTCTTCAACACATACAGGATGCCATGCCCCTGGCTCTGCAGAGT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      GTGATGGACCTTCAGGAGATTTCATACAACAAAGACGATGAGATGGACTATTCTGAGACC
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      CTAAGTAATGCCTTAAATTCGTGTCATGACTTTGCATCTCACTTACAGAGACTCATCCTG
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      ACCCCCTTGTCAGCCCTGCTGTCCTTATTCCCAGGGCCTCACAAGCTCATCCAGAAACGC
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      TATGACAAACTGCTGGATTGCAACAGCTACCTGCAGCGATCAACGGGAGAGGAGTCAGAC
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      TTGGCCAAAAAGGAGTATGAGGCCCTCAACGCCCAGCTTGTGGAGGAGCTCCAGGCATTC
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      AACCAGGCTGCTCGGAAGATTCTGTTGAACTGTCTATGCAGCTTCATTACCCTCCTTAGG
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      GACCTGATGCTCGTGGCACAGCAGGCTTACTCCACACTTGTGCCGATGCCACTGTTGGTT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      TCAAGCATTTCTGAGATTCAGAATCAAGTACTAGAAGAGATCCAAAATTTGAATTGTGTG
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      AAAGAAAACAGTGCCACCTTTATTGAGAGGAAACTCAGTTTTGAAAAGAAGAAACCTGTG
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      CAGATTCTGCCAGAAATGCCACATCAAACTGACATTCATCGCTCCAAACTTCTATCCACA
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      TATAGTGCAGAGGAACTCTATCAAGCTAAGCGCAAGTGCAATGCTACACAAGAATATGAC
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      ATCAATCTTCTGGAAGGAGACTTGGTGGCTGTGATAGAACAGAAAGATCCACTGGGGAGT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      ACAAGCAGGTGGCTTGTGGACACAGGAAATGTGAAAGGATATGTTTATTCCTCCTTCCTA
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      AAACCCTACAATCCAGCAAAAATGCAGAAAGTGGATGCTGAGAACAGGTTCTGTGACGAT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      GATTTTGAGAACATCAGCCTCTTCGTGTCTTCACGGCCAGCTAGTGACAGTGTCACAGGC
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      ACCTCAGAAAGCAGCATTGGTGATAGCAGCTCATCTCTTAGTGGCACATGTGGAAAGTTT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      GAAACAAATGGTACTGATGTTGACAGTTTTCAAGAAGTAGACGAACAGATTTTCTATGCA
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      GTTCATGCTTTTCAAGCACGGAGTGACCATGAACTCAGCCTTCAGGAATACCAGAGAGTT
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------------
NM_001242729.1      CATATACTCAGGTTTTGTGACCTAAGTGGCAATAAAGAGTGGTGGTTAGCTGAAGCTCAA
NM_017700.1         ------------------------------------------------------------
                                                                                

HsCD00508385        ------------------------------------------------------
NM_001242729.1      GGGCAGAAAGGATACGTGCCAGCTAACTACCTTGGAAAGATGACTTATGCTTAA
NM_017700.1         ------------------------------------------------------
                                                                          

Clone

Cloning Information : 665700
Original Clone ID : ccsbBroadEn_03467

Organism


TAX_ID : 9606
Species Specific ID: 54848

Genome

Chromosome : 4
Map Location : 4q24
Ensembl : ENSG00000236699

Reagents

Labome : ARHGEF38-antibody