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UBE2J2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UBE2J2
Gene Name:  ubiquitin-conjugating enzyme E2, J2
Sequence             
Original Clone ID: ccsbBroadEn_09450
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC113430.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00508446

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 778nts         Open reading frame : 1 to 778
Reference Sequence Alignment

ATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCAGAGGCTGAAGCAGGAC
TACCTTCGCATTAAGAAAGACCCGGTGCCTTACATCTGTGCCGAGCCCCTCCCTTCGAAT
ATTCTCGAGTGGCACTATGTCGTCCGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTAT
TATCATGGAAAACTAATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCAGTATCTATATG
ATCACTCCCAACGGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTATCACGGATTTC
CACCCGGACACGTGGAACCCGGCCTGGTCTGTCTCCACCATCCTGACTGGGCTCCTGAGC
TTCATGGTGGAGAAGGGCCCCACCCTGGGCAGTATAGAGACGTCGGACTTCACGAAAAGA
CAACTGGCAGTGCAGAGTTTAGCATTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTT
CCTGAAGTCGTGGAGGAGATTAAACAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGA
CCCCAGACTCTCCCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTTGTCCAGAAC
GGGATTCAGCTGCTCAACGGGCATGCGCCGGGGGCCGTCCCAAACCTCGCAGGGCTCCAG
CAGGCCAACCGGCACCACGGACTCCTGGGTGGCGCCCTGGCGAACTTGTTTGTGATAGTT
GGGTTTGCAGCCTTTGCTTACACGGTCAAGTACGTGCTGAGGAGCATCGCGCAGGAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2J2
Symbol Nomenclature : UBE2J2
SYNONYM : NCUBE-2; NCUBE2; PRO2121
Designation : non-canonical ubiquitin-conjugating enzyme 2|ubiquitin conjugating enzyme 6|ubiquitin conjugating enzyme E2, J2|ubiquitin-conjugating enzyme E2 J2|ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)|yeast UBC6 homolog
Full Nomenclature : ubiquitin-conjugating enzyme E2, J2
GENEID : 118424
Locus Tag : RP5-902P8.8
GenBank Accession : HQ258753
HGNC : 19268
Target GenBank: HQ258753

 

Reference Sequence Alignment

  

HsCD00508446        ATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCAGAGGCTGAAGCAGGAC
NM_194315.1         ATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCAGAGGCTGAAGCAGGAC
NM_058167.2         ATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCAGAGGCTGAAGCAGGAC
XM_005244718.1      ATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCAGAGGCTGAAGCAGGAC
                    ************************************************************

HsCD00508446        TACCTTCGCATTAAGAAAGACCCGGTGCCTTACATCTGTGCCGAGCCCCTCCCTTCGAAT
NM_194315.1         TACCTTCGCATTAAGAAAGACCCGGTGCCTTACATCTGTGCCGAGCCCCTCCCTTCGAAT
NM_058167.2         TACCTTCGCATTAAGAAAGACCCGGTGCCTTACATCTGTGCCGAGCCCCTCCCTTCGAAT
XM_005244718.1      TACCTTCGCATTAAGAAAGACCCGGTGCCTTACATCTGTGCCGAGCCCCTCCCTTCGAAT
                    ************************************************************

HsCD00508446        ATTCTCGAGTG------------------------------------------------G
NM_194315.1         ATTCTCGAGTGGTTCAAGCGATTCTCCTGGCTCAGCCTCCTGAGTAGCTGGGATTACAGG
NM_058167.2         ATTCTCGAGTG------------------------------------------------G
XM_005244718.1      ATTCTCGAGTG------------------------------------------------G
                    ***********                                                *

HsCD00508446        CACTATGTCGTCCGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTATTATCATGGAAAA
NM_194315.1         CACTATGTCGTCCGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTATTATCATGGAAAA
NM_058167.2         CACTATGTCGTCCGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTATTATCATGGAAAA
XM_005244718.1      CACTATGTCGTCCGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTATTATCATGGAAAA
                    ************************************************************

HsCD00508446        CTAATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCAGTATCTATATGATCACTCCCAAC
NM_194315.1         CTAATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCAGTATCTATATGATCACTCCCAAC
NM_058167.2         CTAATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCAGTATCTATATGATCACTCCCAAC
XM_005244718.1      CTAATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCAGTATCTATATGATCACTCCCAAC
                    ************************************************************

HsCD00508446        GGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTATCACGGATTTCCACCCGGACACG
NM_194315.1         GGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTATCACGGATTTCCACCCGGACACG
NM_058167.2         GGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTATCACGGATTTCCACCCGGACACG
XM_005244718.1      GGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTATCACGGATTTCCACCCGGACACG
                    ************************************************************

HsCD00508446        TGGAACCCGGCCTGGTCTGTCTCCACCATCCTGACTGGGCTCCTGAGCTTCATGGTGGAG
NM_194315.1         TGGAACCCGGCCTGGTCTGTCTCCACCATCCTGACTGGGCTCCTGAGCTTCATGGTGGAG
NM_058167.2         TGGAACCCGGCCTGGTCTGTCTCCACCATCCTGACTGGGCTCCTGAGCTTCATGGTGGAG
XM_005244718.1      TGGAACCCGGCCTGGTCTGTCTCCACCATCCTGACTGGGCTCCTGAGCTTCATGGTGGAG
                    ************************************************************

HsCD00508446        AAGGGCCCCACCCTGGGCAGTATAGAGACGTCGGACTTCACGAAAAGACAACTGGCAGTG
NM_194315.1         AAGGGCCCCACCCTGGGCAGTATAGAGACGTCGGACTTCACGAAAAGACAACTGGCAGTG
NM_058167.2         AAGGGCCCCACCCTGGGCAGTATAGAGACGTCGGACTTCACGAAAAGACAACTGGCAGTG
XM_005244718.1      AAGGGCCCCACCCTGGGCAGTATAGAGACGTCGGACTTCACGAAAAGACAACTGGCAGTG
                    ************************************************************

HsCD00508446        CAGAGTTTAGCATTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTCGTG
NM_194315.1         CAGAGTTTAGCATTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTCGTG
NM_058167.2         CAGAGTTTAGCATTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTCGTG
XM_005244718.1      CAGAGTTTAGCATTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTCGTG
                    ************************************************************

HsCD00508446        GAGGAGATTAAACAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGACCCCAGACTCTC
NM_194315.1         GAGGAGATTAAACAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGACCCCAGACTCTC
NM_058167.2         GAGGAGATTAAACAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGACCCCAGACTCTC
XM_005244718.1      GAGGAGATTAAACAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGACCCCAGACTCTC
                    ************************************************************

HsCD00508446        CCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTTGTCCAGAACGGGATTCAGCTG
NM_194315.1         CCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTCGTCCAGAACGGGATTCAGCTG
NM_058167.2         CCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTCGTCCAGAACGGGATTCAGCTG
XM_005244718.1      CCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTCGTCCAGAACGGGATTCAGCTG
                    ************************************** *********************

HsCD00508446        CTCAACGGGCATGCGCCGGGGGCCGTCCCAAACCTCGCAGGGCTCCAGCAGGCCAACCGG
NM_194315.1         CTCAACGGGCATGCGCCGGGGGCCGTCCCAAACCTCGCAGGGCTCCAGCAGGCCAACCGG
NM_058167.2         CTCAACGGGCATGCGCCGGGGGCCGTCCCAAACCTCGCAGGGCTCCAGCAGGCCAACCGG
XM_005244718.1      CTCAACGGGCATGCGCCGGGGGCCGTCCCAAACCTCGCAGGGCTCCAGCAGGCCAACCGG
                    ************************************************************

HsCD00508446        CACCACGGACTCCTGGGTGGCGCCCTGGCGAACTTGTTTGTGATAGTTGGGTTTGCAGCC
NM_194315.1         CACCACGGACTCCTGGGTGGCGCCCTGGCGAACTTGTTTGTGATAGTTGGGTTTGCAGCC
NM_058167.2         CACCACGGACTCCTGGGTGGCGCCCTGGCGAACTTGTTTGTGATAGTTGGGTTTGCAGCC
XM_005244718.1      CACCACGGACTCCTGGGTGGCGCCCTGGCGAACTTGTTTGTGATAGTTGGGTTTGCAGCC
                    ************************************************************

HsCD00508446        TTTGCTTACACGGTCAAGTACGTGCTGAGGAGCATCGCGCAGGAG---
NM_194315.1         TTTGCTTACACGGTCAAGTACGTGCTGAGGAGCATCGCGCAGGAGTGA
NM_058167.2         TTTGCTTACACGGTCAAGTACGTGCTGAGGAGCATCGCGCAGGAGTGA
XM_005244718.1      TTTGCTTACACGGTCAAGTACGTGCTGAGGAGCATCGCGCAGGAGTGA
                    *********************************************   

Pathways

Panther : Parkinson disease
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Clone

Cloning Information : 612537
Original Clone ID : ccsbBroadEn_09450

Organism


TAX_ID : 9606
Species Specific ID: 118424

Genome

Chromosome : 1
Map Location : 1p36.33
Ensembl : ENSG00000160087

Reagents

Labome : UBE2J2-antibody