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MPG (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MPG
Gene Name:  N-methylpurine-DNA glycosylase
Sequence             
Original Clone ID: ccsbBroadEn_01031
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC014991.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00508956

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 880nts         Open reading frame : 1 to 880
Reference Sequence Alignment

ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAAAAGAAGCAGCGACCA
GCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCACCTGCAGAGCAGCCA
CACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGCTGCTTGGGACCGCCC
ACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAGGGCCACCTTACCCGA
CTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGGGCATTTCTGGGACAG
GTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGCATCGTGGAGACCGAG
GCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGCCGGCAGACCCCCCGC
AACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATCATTTACGGCATGTAC
TTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTGCTGCGAGCACTGGAG
CCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTCCGGAAAGGCACCGCC
AGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAGCTGTGCCAGGCCCTG
GCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAAGCTGTATGGCTGGAG
CGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCCCGGGTGGGCGTCGGC
CATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGGGGCAGCCCCTGGGTC
AGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MPG
Symbol Nomenclature : MPG
SYNONYM : AAG; ADPG; APNG; CRA36.1; MDG; Mid1; PIG11; PIG16; anpg
Designation : 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p|3-alkyladenine DNA glycosylase|3-methyladenine DNA glycosidase|CRA36.1 (3-methyl-adenine DNA glycosylase)|DNA-3-methyladenine glycosylase|N-methylpurine-DNA glycosylase, MPG|proliferation-inducing protein 11|proliferation-inducing protein 16
Full Nomenclature : N-methylpurine-DNA glycosylase
GENEID : 4350
Locus Tag : Z69720.1-002
GenBank Accession : DQ895912
HGNC : 7211
MIM : 156565
Vega : OTTHUMG00000047887
Target GenBank: DQ895912

 

Reference Sequence Alignment

  

HsCD00508956        ---------------ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAA
NM_001015054.2      ---------------------------------------------------ATGGGGCAA
NM_001015052.2      ---------------ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAA
NM_002434.3         ATGGTCACCCCCGCTTTGCAGATGAAGAAACCAAAGCAGTTTTGCCGACGGATGGGGCAA
                                                                       *********

HsCD00508956        AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_001015054.2      AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_001015052.2      AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_002434.3         AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
                    ************************************************************

HsCD00508956        CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_001015054.2      CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_001015052.2      CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_002434.3         CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
                    ************************************************************

HsCD00508956        TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_001015054.2      TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_001015052.2      TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_002434.3         TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
                    ************************************************************

HsCD00508956        GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_001015054.2      GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_001015052.2      GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_002434.3         GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
                    ************************************************************

HsCD00508956        GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_001015054.2      GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_001015052.2      GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_002434.3         GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
                    ************************************************************

HsCD00508956        ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_001015054.2      ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_001015052.2      ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_002434.3         ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
                    ************************************************************

HsCD00508956        CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_001015054.2      CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_001015052.2      CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_002434.3         CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
                    ************************************************************

HsCD00508956        ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_001015054.2      ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_001015052.2      ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_002434.3         ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
                    ************************************************************

HsCD00508956        CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_001015054.2      CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_001015052.2      CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_002434.3         CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
                    ************************************************************

HsCD00508956        CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_001015054.2      CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_001015052.2      CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_002434.3         CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
                    ************************************************************

HsCD00508956        CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_001015054.2      CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_001015052.2      CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_002434.3         CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
                    ************************************************************

HsCD00508956        GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_001015054.2      GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_001015052.2      GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_002434.3         GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
                    ************************************************************

HsCD00508956        CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_001015054.2      CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_001015052.2      CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_002434.3         CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
                    ************************************************************

HsCD00508956        GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCC---
NM_001015054.2      GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
NM_001015052.2      GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
NM_002434.3         GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
                    ******************************************************   

Pathways

NCI : Validated nuclear estrogen receptor alpha network
Reactome : Base Excision Repair
Reactome : Base-Excision Repair, AP Site Formation
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : Cleavage of the damaged purine
Reactome : DNA Repair
Reactome : Depurination
Reactome : Displacement of DNA glycosylase by APE1
Reactome : Recognition and association of DNA glycosylase with site containing an affected purine
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
Reactome : Resolution of Abasic Sites (AP sites)

Protein

HPRD : 06766

Clone

Cloning Information : 613893
Original Clone ID : ccsbBroadEn_01031

Organism


TAX_ID : 9606
Species Specific ID: 4350

Genome

Chromosome : 16
Map Location : 16p13.3
Ensembl : ENSG00000103152

Reagents

Labome : MPG-antibody