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CA10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CA10
Gene Name:  carbonic anhydrase X
Sequence             
Original Clone ID: ccsbBroadEn_03757
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC020577.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00509964

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 985nts         Open reading frame : 1 to 985
Reference Sequence Alignment

ATGGAAATAGTCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCA
GCTCAACAGAATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAG
GGAAGCTTTGTTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGC
TCTGTGGGGAAACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCC
TTTCTGACACCTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAAC
ACTGGAAGACACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGG
CCCATGACATACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGC
CAAGGGTCGGAGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCAC
TATAACCATGAGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTG
GTAGTTTCTATATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTC
AACAGAGATACTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTT
AATATAGAGGAACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACT
ATCCCACCCTGCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACC
AGGATGCAGATGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTG
AGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAAT
ATCAACTTCAGTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTAT
AGAGTAAATGAATGGCTCCTCAAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CA10
Symbol Nomenclature : CA10
SYNONYM : CA-RPX; CARPX; HUCEP-15
Designation : CA-RP X|CARP X|carbonic anhydrase-related protein 10|carbonic anhydrase-related protein X|cerebral protein 15|cerebral protein-15
Full Nomenclature : carbonic anhydrase X
GENEID : 56934
Locus Tag : hucep-15
GenBank Accession : JF432658
HGNC : 1369
MIM : 604642
Vega : OTTHUMG00000177544
Target GenBank: JF432658

 

Reference Sequence Alignment

  

HsCD00509964        ATGGAAATAGTCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCA
NM_020178.4         ATGGAAATAGTCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCA
NM_001082534.1      ATGGAAATAGTCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCA
NM_001082533.1      ATGGAAATAGTCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCA
                    ************************************************************

HsCD00509964        GCTCAACAGAATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAG
NM_020178.4         GCTCAACAGAATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAG
NM_001082534.1      GCTCAACAGAATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAG
NM_001082533.1      GCTCAACAGAATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAG
                    ************************************************************

HsCD00509964        GGAAGCTTTGTTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGC
NM_020178.4         GGAAGCTTTGTTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGC
NM_001082534.1      GGAAGCTTTGTTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGC
NM_001082533.1      GGAAGCTTTGTTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGC
                    ************************************************************

HsCD00509964        TCTGTGGGGAAACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCC
NM_020178.4         TCTGTGGGGAAACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCC
NM_001082534.1      TCTGTGGGGAAACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCC
NM_001082533.1      TCTGTGGGGAAACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCC
                    ************************************************************

HsCD00509964        TTTCTGACACCTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAAC
NM_020178.4         TTTCTGACACCTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAAC
NM_001082534.1      TTTCTGACACCTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAAC
NM_001082533.1      TTTCTGACACCTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAAC
                    ************************************************************

HsCD00509964        ACTGGAAGACACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGG
NM_020178.4         ACTGGAAGACACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGG
NM_001082534.1      ACTGGAAGACACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGG
NM_001082533.1      ACTGGAAGACACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGG
                    ************************************************************

HsCD00509964        CCCATGACATACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGC
NM_020178.4         CCCATGACATACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGC
NM_001082534.1      CCCATGACATACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGC
NM_001082533.1      CCCATGACATACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGC
                    ************************************************************

HsCD00509964        CAAGGGTCGGAGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCAC
NM_020178.4         CAAGGGTCGGAGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCAC
NM_001082534.1      CAAGGGTCGGAGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCAC
NM_001082533.1      CAAGGGTCGGAGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCAC
                    ************************************************************

HsCD00509964        TATAACCATGAGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTG
NM_020178.4         TATAACCATGAGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTG
NM_001082534.1      TATAACCATGAGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTG
NM_001082533.1      TATAACCATGAGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTG
                    ************************************************************

HsCD00509964        GTAGTTTCTATATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTC
NM_020178.4         GTAGTTTCTATATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTC
NM_001082534.1      GTAGTTTCTATATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTC
NM_001082533.1      GTAGTTTCTATATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTC
                    ************************************************************

HsCD00509964        AACAGAGATACTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTT
NM_020178.4         AACAGAGATACTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTT
NM_001082534.1      AACAGAGATACTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTT
NM_001082533.1      AACAGAGATACTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTT
                    ************************************************************

HsCD00509964        AATATAGAGGAACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACT
NM_020178.4         AATATAGAGGAACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACT
NM_001082534.1      AATATAGAGGAACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACT
NM_001082533.1      AATATAGAGGAACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACT
                    ************************************************************

HsCD00509964        ATCCCACCCTGCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACC
NM_020178.4         ATCCCACCCTGCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACC
NM_001082534.1      ATCCCACCCTGCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACC
NM_001082533.1      ATCCCACCCTGCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACC
                    ************************************************************

HsCD00509964        AGGATGCAGATGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTG
NM_020178.4         AGGATGCAGATGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTG
NM_001082534.1      AGGATGCAGATGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTG
NM_001082533.1      AGGATGCAGATGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTG
                    ************************************************************

HsCD00509964        AGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAAT
NM_020178.4         AGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAAT
NM_001082534.1      AGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAAT
NM_001082533.1      AGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAAT
                    ************************************************************

HsCD00509964        ATCAACTTCAGTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTAT
NM_020178.4         ATCAACTTCAGTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTAT
NM_001082534.1      ATCAACTTCAGTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTAT
NM_001082533.1      ATCAACTTCAGTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTAT
                    ************************************************************

HsCD00509964        AGAGTAAATGAATGGCTCCTCAAG---
NM_020178.4         AGAGTAAATGAATGGCTCCTCAAGTAG
NM_001082534.1      AGAGTAAATGAATGGCTCCTCAAGTAG
NM_001082533.1      AGAGTAAATGAATGGCTCCTCAAGTAG
                    ************************   

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
HPRD : 05224

Clone

Cloning Information : 609429
Original Clone ID : ccsbBroadEn_03757

Organism


TAX_ID : 9606
Species Specific ID: 56934

Genome

Chromosome : 17
Map Location : 17q21.33
Ensembl : ENSG00000154975

Reagents

Labome : CA10-antibody