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P2RY14 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  P2RY14
Gene Name:  purinergic receptor P2Y, G-protein coupled, 14
Sequence             
Original Clone ID: ccsbBroadEn_07513
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC034989.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00510035

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1015nts         Open reading frame : 1 to 1015
Reference Sequence Alignment

ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTGAGAGTTTCATCATCTATCTC
AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : P2RY14
Symbol Nomenclature : P2RY14
SYNONYM : BPR105; GPR105; P2Y14
Designation : G protein coupled receptor for UDP-glucose|G protein-coupled receptor 105|G-protein coupled receptor 105|P2Y purinoceptor 14|P2Y(14) receptor|P2Y14 receptor|UDP-glucose receptor
Full Nomenclature : purinergic receptor P2Y, G-protein coupled, 14
GENEID : 9934
HGNC : 16442
MIM : 610116
Vega : OTTHUMG00000159859
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00510035        ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
NM_014879.3         ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
XM_005247921.1      ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
NM_001081455.1      ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
XM_005247923.1      ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATC
                    ************************************************************

HsCD00510035        ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
NM_014879.3         ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
XM_005247921.1      ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
NM_001081455.1      ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
XM_005247923.1      ACTCAGCAGATCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAAT
                    ************************************************************

HsCD00510035        GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTGAGAGTTTCATCATCTATCTC
NM_014879.3         GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
XM_005247921.1      GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
NM_001081455.1      GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
XM_005247923.1      GGAGTGTCAGGATGGATATTCTTTTACGTGCCCAGCTCTAAGAGTTTCATCATCTATCTC
                    ***************************************.********************

HsCD00510035        AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
NM_014879.3         AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
XM_005247921.1      AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
NM_001081455.1      AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
XM_005247923.1      AAGAACATTGTTATTGCTGACTTTGTGATGAGCCTGACTTTTCCTTTCAAGATCCTTGGT
                    ************************************************************

HsCD00510035        GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
NM_014879.3         GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
XM_005247921.1      GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
NM_001081455.1      GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
XM_005247923.1      GACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGTGCAGGGTCTCTGCCGTGCTC
                    ************************************************************

HsCD00510035        TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
NM_014879.3         TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
XM_005247921.1      TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
NM_001081455.1      TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
XM_005247923.1      TTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTTTGACAGATAT
                    ************************************************************

HsCD00510035        TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
NM_014879.3         TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
XM_005247921.1      TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
NM_001081455.1      TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
XM_005247923.1      TATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT
                    ************************************************************

HsCD00510035        CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
NM_014879.3         CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
XM_005247921.1      CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
NM_001081455.1      CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
XM_005247923.1      CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACC
                    ************************************************************

HsCD00510035        AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
NM_014879.3         AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
XM_005247921.1      AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
NM_001081455.1      AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
XM_005247923.1      AACCAGAGTGTTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGA
                    ************************************************************

HsCD00510035        CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
NM_014879.3         CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
XM_005247921.1      CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
NM_001081455.1      CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
XM_005247923.1      CGGAAGTGGCACAAAGCATCAAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTT
                    ************************************************************

HsCD00510035        TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
NM_014879.3         TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
XM_005247921.1      TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
NM_001081455.1      TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
XM_005247923.1      TTGTTAATCGTTTTCTATACTGCTATCACAAAGAAAATCTTTAAGTCCCACCTTAAGTCA
                    ************************************************************

HsCD00510035        AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
NM_014879.3         AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
XM_005247921.1      AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
NM_001081455.1      AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
XM_005247923.1      AGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCAACATATTCAGCATCGTGTTT
                    ************************************************************

HsCD00510035        GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
NM_014879.3         GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
XM_005247921.1      GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
NM_001081455.1      GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
XM_005247923.1      GTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACACAAAGAGTCAG
                    ************************************************************

HsCD00510035        ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
NM_014879.3         ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
XM_005247921.1      ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
NM_001081455.1      ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
XM_005247923.1      ACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT
                    ************************************************************

HsCD00510035        CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
NM_014879.3         CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
XM_005247921.1      CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
NM_001081455.1      CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
XM_005247923.1      CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAG
                    ************************************************************

HsCD00510035        CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
NM_014879.3         CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
XM_005247921.1      CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
NM_001081455.1      CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
XM_005247923.1      CCGTTTAGGGAAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTA
                    ************************************************************

HsCD00510035        GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTG---
NM_014879.3         GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
XM_005247921.1      GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
NM_001081455.1      GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
XM_005247923.1      GACATTTCCAGAATCAAAAGAGGAAATACAACACTTGAAAGCACAGATACTTTGTGA
                    ******************************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 08820

Clone

Cloning Information : 608936
Original Clone ID : ccsbBroadEn_07513

Organism


TAX_ID : 9606
Species Specific ID: 9934

Genome

Chromosome : 3
Map Location : 3q24-q25.1
Ensembl : ENSG00000174944

Reagents

Labome : P2RY14-antibody