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LGALS9B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS9C
Gene Name:  lectin, galactoside-binding, soluble, 9C
Sequence             
Original Clone ID: ccsbBroadEn_09955
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC105943.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00510325

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1069nts         Open reading frame : 1 to 1069
Reference Sequence Alignment

ATGGCCTTCAGCGGTTCCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTCC
AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
AGATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGCCTGCCCCGAAAAATGCCC
TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS9C
Symbol Nomenclature : LGALS9C
Designation : gal-9C|galectin 9 like|galectin-9-like protein B|galectin-9C|lectin, galactoside-binding, soluble, 9 (galectin 9) pseudogene
Full Nomenclature : lectin, galactoside-binding, soluble, 9C
GENEID : 654346
HGNC : 33874
Vega : OTTHUMG00000059251
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00510325        ATGGCCTTCAGCGGTTCCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
NM_009587.2         ATGGCCTTCAGCGGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACT
NM_001040078.2      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
XM_005256769.1      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
NM_001042685.1      ATGGCCTTCAGCGGTTCCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
                    **************** ************ ***** ***** ******************

HsCD00510325        ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTCC
NM_009587.2         ATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCC
NM_001040078.2      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
XM_005256769.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
NM_001042685.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTCC
                    ** *****.*************** ********************.************ *

HsCD00510325        AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
NM_009587.2         AGTGGAACCAGGTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTC
NM_001040078.2      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
XM_005256769.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
NM_001042685.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
                    *********************.********** ************** ************

HsCD00510325        CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
NM_009587.2         CACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGA
NM_001040078.2      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
XM_005256769.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
NM_001042685.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
                    *********************** ******** ***********************.***

HsCD00510325        AGATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
NM_009587.2         AGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGAC
NM_001040078.2      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
XM_005256769.1      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
NM_001042685.1      AGATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
                    * .********************** .******** ************************

HsCD00510325        CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
NM_009587.2         CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTG
NM_001040078.2      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
XM_005256769.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
NM_001042685.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
                    ************************************************* **********

HsCD00510325        CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_009587.2         CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_001040078.2      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
XM_005256769.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_001042685.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
                    ************************************************************

HsCD00510325        CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
NM_009587.2         CAGCTGTCCTACATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCC
NM_001040078.2      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
XM_005256769.1      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
NM_001042685.1      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCACAGTCCCCGTTCAGCCTGCCTTCTCC
                    ************************** ******.******* ******************

HsCD00510325        ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_009587.2         ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_001040078.2      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
XM_005256769.1      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_001042685.1      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
                    ************************************************************

HsCD00510325        CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
NM_009587.2         CCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTG
NM_001040078.2      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
XM_005256769.1      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
NM_001042685.1      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
                    ******.***** *************.*****************************.***

HsCD00510325        CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
NM_009587.2         CAGAGCGCCCCTGGACAGATGTTCTCTACT---CCCGCCATCCCACCTATGATGTACCCC
NM_001040078.2      CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
XM_005256769.1      CAGAGTGCCTCTGGACAGATGTTCTCTAC---TCCCGCCATCCCACCTATGATGTACCCC
NM_001042685.1      CAGAGCGCCTCTGGACAGATGTTCTCTACT---CCCGCCATCCCACCTATGATGTACCCC
                    ***** *** *****************..    ***************************

HsCD00510325        CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
NM_009587.2         CACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAG
NM_001040078.2      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
XM_005256769.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
NM_001042685.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
                    ***** ******************************* **********************

HsCD00510325        TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_009587.2         TCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_001040078.2      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
XM_005256769.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_001042685.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
                    ******.*****************************************************

HsCD00510325        TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
NM_009587.2         TCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGC
NM_001040078.2      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
XM_005256769.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
NM_001042685.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
                    *******.****************.********************************** 

HsCD00510325        AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGCCTGCCCCGAAAAATGCCC
NM_009587.2         AACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_001040078.2      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
XM_005256769.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_001042685.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
                    ************.******* ******************** ******************

HsCD00510325        TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_009587.2         TTCGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_001040078.2      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
XM_005256769.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_001042685.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
                    ********:**************.************************************

HsCD00510325        GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_009587.2         GCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_001040078.2      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
XM_005256769.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_001042685.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
                    ****************** *****************************************

HsCD00510325        AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACA---
NM_009587.2         AACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAG
NM_001040078.2      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
XM_005256769.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
NM_001042685.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
                    ****.************ ******************** *********   

Clone

Cloning Information : 610166
Original Clone ID : ccsbBroadEn_09955

Organism


TAX_ID : 9606
Species Specific ID: 284194

Genome

Chromosome : 17
Map Location : 17p11.2
Ensembl : ENSG00000171916

Reagents

Labome : LGALS9B-antibody