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LYSMD1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LYSMD1
Gene Name:  LysM, putative peptidoglycan-binding, domain containing 1
Sequence             
Original Clone ID: ccsbBroadEn_05591
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC031649.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00510515

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 682nts        
Open reading frame : 1 to 682
Reference Sequence Alignment

ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
GAGGATGAAATCTTCAAACTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LYSMD1
Symbol Nomenclature : LYSMD1
SYNONYM : RP11-68I18.5
Designation : lysM and putative peptidoglycan-binding domain-containing protein 1
Full Nomenclature : LysM, putative peptidoglycan-binding, domain containing 1
GENEID : 388695
Locus Tag : SB145
GenBank Accession : HQ447451
HGNC : 32070
Vega : OTTHUMG00000012260
Target GenBank: HQ447451

 

Reference Sequence Alignment

  

HsCD00510515      ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
NM_212551.4       ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
                  ************************************************************

HsCD00510515      GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
NM_212551.4       GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
                  ************************************************************

HsCD00510515      GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
NM_212551.4       GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
                  ************************************************************

HsCD00510515      ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
NM_212551.4       ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
                  ************************************************************

HsCD00510515      ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
NM_212551.4       ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
                  ************************************************************

HsCD00510515      GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
NM_212551.4       GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
                  ************************************************************

HsCD00510515      ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
NM_212551.4       ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
                  ************************************************************

HsCD00510515      CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
NM_212551.4       CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
                  ************************************************************

HsCD00510515      CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
NM_212551.4       CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
                  ************************************************************

HsCD00510515      AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
NM_212551.4       AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
                  ************************************************************

HsCD00510515      GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
NM_212551.4       GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
                  ************************************************************

HsCD00510515      GAGGATGAAATCTTCAAACTC---
NM_212551.4       GAGGATGAAATCTTCAAACTCTGA
                  *********************   

Protein

SMART domain : LysM : Lysin motif
HPRD : 18017

Clone

Cloning Information : 609792
Original Clone ID : ccsbBroadEn_05591

Organism


TAX_ID : 9606
Species Specific ID: 388695

Genome

Chromosome : 1
Map Location : 1q21.3
Ensembl : ENSG00000163155

Reagents

Labome : LYSMD1-antibody